Cargando…

Comparison of the Effects of Microbial Inoculants on Fermentation Quality and Microbiota in Napier Grass (Pennisetum purpureum) and Corn (Zea mays L.) Silage

Forage preservation for livestock feeding is usually done by drying the plant material and storing it as hay or ensiling it into silage. During the ensiling process, the pH in the system is lowered by the activities of lactic acid-producing bacteria (LAB), inhibiting the growth of spoilage microorga...

Descripción completa

Detalles Bibliográficos
Autores principales: Jaipolsaen, Narongrit, Sangsritavong, Siwat, Uengwetwanit, Tanaporn, Angthong, Pacharaporn, Plengvidhya, Vethachai, Rungrassamee, Wanilada, Yammuenart, Saowaluck
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8811201/
https://www.ncbi.nlm.nih.gov/pubmed/35126328
http://dx.doi.org/10.3389/fmicb.2021.784535
_version_ 1784644381444145152
author Jaipolsaen, Narongrit
Sangsritavong, Siwat
Uengwetwanit, Tanaporn
Angthong, Pacharaporn
Plengvidhya, Vethachai
Rungrassamee, Wanilada
Yammuenart, Saowaluck
author_facet Jaipolsaen, Narongrit
Sangsritavong, Siwat
Uengwetwanit, Tanaporn
Angthong, Pacharaporn
Plengvidhya, Vethachai
Rungrassamee, Wanilada
Yammuenart, Saowaluck
author_sort Jaipolsaen, Narongrit
collection PubMed
description Forage preservation for livestock feeding is usually done by drying the plant material and storing it as hay or ensiling it into silage. During the ensiling process, the pH in the system is lowered by the activities of lactic acid-producing bacteria (LAB), inhibiting the growth of spoilage microorganisms and maintaining the quality of the ensiled product. To improve this process, inoculation of LAB could be used as starter cultures to shorten the ensiling time and control the fermentation process. Here, we compared fermentation quality and bacterial dynamics in two plant materials, whole-plant corn (Zea mays L.) and Napier grass (Pennisetum purpureum), with and without starter inoculation. The efficacy of Lactobacillus plantarum, L. brevis, and Pediococcus pentosaceus as starter cultures were also compared in the ensiling system. In whole-plant corn, pH decreased significantly, while lactic acid content increased significantly on Day 3 in both the non-inoculated and LAB-inoculated groups. Prior to ensiling, the predominant LAB bacteria were Weissella, Enterococcus, and Lactococcus, which shifted to Lactobacillus during ensiling of whole-plant corn in both the non-inoculated and LAB inoculated groups. Interestingly, the epiphytic LAB associated with Napier grass were much lower than those of whole-plant corn before ensiling. Consequently, the fermentation quality of Napier grass was improved by the addition of LAB inoculants, especially L. plantarum and a combination of all three selected LAB strains showed better fermentation quality than the non-inoculated control. Therefore, the different abundance and diversity of epiphytic LAB in plant raw materials could be one of the most important factors determining whether LAB starter cultures would be necessary for silage fermentation.
format Online
Article
Text
id pubmed-8811201
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88112012022-02-04 Comparison of the Effects of Microbial Inoculants on Fermentation Quality and Microbiota in Napier Grass (Pennisetum purpureum) and Corn (Zea mays L.) Silage Jaipolsaen, Narongrit Sangsritavong, Siwat Uengwetwanit, Tanaporn Angthong, Pacharaporn Plengvidhya, Vethachai Rungrassamee, Wanilada Yammuenart, Saowaluck Front Microbiol Microbiology Forage preservation for livestock feeding is usually done by drying the plant material and storing it as hay or ensiling it into silage. During the ensiling process, the pH in the system is lowered by the activities of lactic acid-producing bacteria (LAB), inhibiting the growth of spoilage microorganisms and maintaining the quality of the ensiled product. To improve this process, inoculation of LAB could be used as starter cultures to shorten the ensiling time and control the fermentation process. Here, we compared fermentation quality and bacterial dynamics in two plant materials, whole-plant corn (Zea mays L.) and Napier grass (Pennisetum purpureum), with and without starter inoculation. The efficacy of Lactobacillus plantarum, L. brevis, and Pediococcus pentosaceus as starter cultures were also compared in the ensiling system. In whole-plant corn, pH decreased significantly, while lactic acid content increased significantly on Day 3 in both the non-inoculated and LAB-inoculated groups. Prior to ensiling, the predominant LAB bacteria were Weissella, Enterococcus, and Lactococcus, which shifted to Lactobacillus during ensiling of whole-plant corn in both the non-inoculated and LAB inoculated groups. Interestingly, the epiphytic LAB associated with Napier grass were much lower than those of whole-plant corn before ensiling. Consequently, the fermentation quality of Napier grass was improved by the addition of LAB inoculants, especially L. plantarum and a combination of all three selected LAB strains showed better fermentation quality than the non-inoculated control. Therefore, the different abundance and diversity of epiphytic LAB in plant raw materials could be one of the most important factors determining whether LAB starter cultures would be necessary for silage fermentation. Frontiers Media S.A. 2022-01-20 /pmc/articles/PMC8811201/ /pubmed/35126328 http://dx.doi.org/10.3389/fmicb.2021.784535 Text en Copyright © 2022 Jaipolsaen, Sangsritavong, Uengwetwanit, Angthong, Plengvidhya, Rungrassamee and Yammuenart. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Jaipolsaen, Narongrit
Sangsritavong, Siwat
Uengwetwanit, Tanaporn
Angthong, Pacharaporn
Plengvidhya, Vethachai
Rungrassamee, Wanilada
Yammuenart, Saowaluck
Comparison of the Effects of Microbial Inoculants on Fermentation Quality and Microbiota in Napier Grass (Pennisetum purpureum) and Corn (Zea mays L.) Silage
title Comparison of the Effects of Microbial Inoculants on Fermentation Quality and Microbiota in Napier Grass (Pennisetum purpureum) and Corn (Zea mays L.) Silage
title_full Comparison of the Effects of Microbial Inoculants on Fermentation Quality and Microbiota in Napier Grass (Pennisetum purpureum) and Corn (Zea mays L.) Silage
title_fullStr Comparison of the Effects of Microbial Inoculants on Fermentation Quality and Microbiota in Napier Grass (Pennisetum purpureum) and Corn (Zea mays L.) Silage
title_full_unstemmed Comparison of the Effects of Microbial Inoculants on Fermentation Quality and Microbiota in Napier Grass (Pennisetum purpureum) and Corn (Zea mays L.) Silage
title_short Comparison of the Effects of Microbial Inoculants on Fermentation Quality and Microbiota in Napier Grass (Pennisetum purpureum) and Corn (Zea mays L.) Silage
title_sort comparison of the effects of microbial inoculants on fermentation quality and microbiota in napier grass (pennisetum purpureum) and corn (zea mays l.) silage
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8811201/
https://www.ncbi.nlm.nih.gov/pubmed/35126328
http://dx.doi.org/10.3389/fmicb.2021.784535
work_keys_str_mv AT jaipolsaennarongrit comparisonoftheeffectsofmicrobialinoculantsonfermentationqualityandmicrobiotainnapiergrasspennisetumpurpureumandcornzeamayslsilage
AT sangsritavongsiwat comparisonoftheeffectsofmicrobialinoculantsonfermentationqualityandmicrobiotainnapiergrasspennisetumpurpureumandcornzeamayslsilage
AT uengwetwanittanaporn comparisonoftheeffectsofmicrobialinoculantsonfermentationqualityandmicrobiotainnapiergrasspennisetumpurpureumandcornzeamayslsilage
AT angthongpacharaporn comparisonoftheeffectsofmicrobialinoculantsonfermentationqualityandmicrobiotainnapiergrasspennisetumpurpureumandcornzeamayslsilage
AT plengvidhyavethachai comparisonoftheeffectsofmicrobialinoculantsonfermentationqualityandmicrobiotainnapiergrasspennisetumpurpureumandcornzeamayslsilage
AT rungrassameewanilada comparisonoftheeffectsofmicrobialinoculantsonfermentationqualityandmicrobiotainnapiergrasspennisetumpurpureumandcornzeamayslsilage
AT yammuenartsaowaluck comparisonoftheeffectsofmicrobialinoculantsonfermentationqualityandmicrobiotainnapiergrasspennisetumpurpureumandcornzeamayslsilage