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Determination of Hepatic Iron Deposition in Drug-Induced Liver Fibrosis in Rats by Confocal Micro-XRF Spectrometry

[Image: see text] Liver fibrosis is the intermediate process and inevitable stage of the development of chronic liver disease into cirrhosis. Reducing the degree of liver fibrosis plays an extremely important role in treating chronic liver disease and preventing liver cirrhosis and liver cancer. The...

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Autores principales: Xu, Qianqian, Xia, Wenjing, Zhou, Lazhen, Zou, Zhengwei, Li, Qiuxia, Deng, Lijun, Wu, Sha, Wang, Tao, Cui, Jingduo, Liu, Zhiguo, Sun, Tianxi, Ye, Junsong, Li, Fangzuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8811927/
https://www.ncbi.nlm.nih.gov/pubmed/35128282
http://dx.doi.org/10.1021/acsomega.1c06476
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author Xu, Qianqian
Xia, Wenjing
Zhou, Lazhen
Zou, Zhengwei
Li, Qiuxia
Deng, Lijun
Wu, Sha
Wang, Tao
Cui, Jingduo
Liu, Zhiguo
Sun, Tianxi
Ye, Junsong
Li, Fangzuo
author_facet Xu, Qianqian
Xia, Wenjing
Zhou, Lazhen
Zou, Zhengwei
Li, Qiuxia
Deng, Lijun
Wu, Sha
Wang, Tao
Cui, Jingduo
Liu, Zhiguo
Sun, Tianxi
Ye, Junsong
Li, Fangzuo
author_sort Xu, Qianqian
collection PubMed
description [Image: see text] Liver fibrosis is the intermediate process and inevitable stage of the development of chronic liver disease into cirrhosis. Reducing the degree of liver fibrosis plays an extremely important role in treating chronic liver disease and preventing liver cirrhosis and liver cancer. The formation of liver fibrosis is affected by iron deposition to a certain extent, and excessive iron deposition further induces liver cirrhosis and liver cancer. Herein, confocal microbeam X-ray fluorescence (μ-XRF) was used to determine the intensity and biodistribution of iron deposition at different time points in the process of liver fibrosis induced by thioacetamide (TAA) in rats. To our best knowledge, this is the first study using confocal μ-XRF to analyze hepatic iron deposition in hepatic fibrosis. The results showed that there are minor and trace elements such as iron, potassium, and zinc in the liver of rats. Continuous injection of TAA solution resulted in increasing liver iron deposition over time. The intensity of iron deposition in liver tissue was also significantly reduced after bone mesenchymal stem cells (BMSCs) were injected. These findings indicated that confocal μ-XRF can be used as a nondestructive and quantitative method of evaluating hepatic iron deposition in hepatic fibrosis, and iron deposition may play an important role in the progression of hepatic fibrosis induced by TAA.
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spelling pubmed-88119272022-02-04 Determination of Hepatic Iron Deposition in Drug-Induced Liver Fibrosis in Rats by Confocal Micro-XRF Spectrometry Xu, Qianqian Xia, Wenjing Zhou, Lazhen Zou, Zhengwei Li, Qiuxia Deng, Lijun Wu, Sha Wang, Tao Cui, Jingduo Liu, Zhiguo Sun, Tianxi Ye, Junsong Li, Fangzuo ACS Omega [Image: see text] Liver fibrosis is the intermediate process and inevitable stage of the development of chronic liver disease into cirrhosis. Reducing the degree of liver fibrosis plays an extremely important role in treating chronic liver disease and preventing liver cirrhosis and liver cancer. The formation of liver fibrosis is affected by iron deposition to a certain extent, and excessive iron deposition further induces liver cirrhosis and liver cancer. Herein, confocal microbeam X-ray fluorescence (μ-XRF) was used to determine the intensity and biodistribution of iron deposition at different time points in the process of liver fibrosis induced by thioacetamide (TAA) in rats. To our best knowledge, this is the first study using confocal μ-XRF to analyze hepatic iron deposition in hepatic fibrosis. The results showed that there are minor and trace elements such as iron, potassium, and zinc in the liver of rats. Continuous injection of TAA solution resulted in increasing liver iron deposition over time. The intensity of iron deposition in liver tissue was also significantly reduced after bone mesenchymal stem cells (BMSCs) were injected. These findings indicated that confocal μ-XRF can be used as a nondestructive and quantitative method of evaluating hepatic iron deposition in hepatic fibrosis, and iron deposition may play an important role in the progression of hepatic fibrosis induced by TAA. American Chemical Society 2022-01-24 /pmc/articles/PMC8811927/ /pubmed/35128282 http://dx.doi.org/10.1021/acsomega.1c06476 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Xu, Qianqian
Xia, Wenjing
Zhou, Lazhen
Zou, Zhengwei
Li, Qiuxia
Deng, Lijun
Wu, Sha
Wang, Tao
Cui, Jingduo
Liu, Zhiguo
Sun, Tianxi
Ye, Junsong
Li, Fangzuo
Determination of Hepatic Iron Deposition in Drug-Induced Liver Fibrosis in Rats by Confocal Micro-XRF Spectrometry
title Determination of Hepatic Iron Deposition in Drug-Induced Liver Fibrosis in Rats by Confocal Micro-XRF Spectrometry
title_full Determination of Hepatic Iron Deposition in Drug-Induced Liver Fibrosis in Rats by Confocal Micro-XRF Spectrometry
title_fullStr Determination of Hepatic Iron Deposition in Drug-Induced Liver Fibrosis in Rats by Confocal Micro-XRF Spectrometry
title_full_unstemmed Determination of Hepatic Iron Deposition in Drug-Induced Liver Fibrosis in Rats by Confocal Micro-XRF Spectrometry
title_short Determination of Hepatic Iron Deposition in Drug-Induced Liver Fibrosis in Rats by Confocal Micro-XRF Spectrometry
title_sort determination of hepatic iron deposition in drug-induced liver fibrosis in rats by confocal micro-xrf spectrometry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8811927/
https://www.ncbi.nlm.nih.gov/pubmed/35128282
http://dx.doi.org/10.1021/acsomega.1c06476
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