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Determination of Hepatic Iron Deposition in Drug-Induced Liver Fibrosis in Rats by Confocal Micro-XRF Spectrometry
[Image: see text] Liver fibrosis is the intermediate process and inevitable stage of the development of chronic liver disease into cirrhosis. Reducing the degree of liver fibrosis plays an extremely important role in treating chronic liver disease and preventing liver cirrhosis and liver cancer. The...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8811927/ https://www.ncbi.nlm.nih.gov/pubmed/35128282 http://dx.doi.org/10.1021/acsomega.1c06476 |
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author | Xu, Qianqian Xia, Wenjing Zhou, Lazhen Zou, Zhengwei Li, Qiuxia Deng, Lijun Wu, Sha Wang, Tao Cui, Jingduo Liu, Zhiguo Sun, Tianxi Ye, Junsong Li, Fangzuo |
author_facet | Xu, Qianqian Xia, Wenjing Zhou, Lazhen Zou, Zhengwei Li, Qiuxia Deng, Lijun Wu, Sha Wang, Tao Cui, Jingduo Liu, Zhiguo Sun, Tianxi Ye, Junsong Li, Fangzuo |
author_sort | Xu, Qianqian |
collection | PubMed |
description | [Image: see text] Liver fibrosis is the intermediate process and inevitable stage of the development of chronic liver disease into cirrhosis. Reducing the degree of liver fibrosis plays an extremely important role in treating chronic liver disease and preventing liver cirrhosis and liver cancer. The formation of liver fibrosis is affected by iron deposition to a certain extent, and excessive iron deposition further induces liver cirrhosis and liver cancer. Herein, confocal microbeam X-ray fluorescence (μ-XRF) was used to determine the intensity and biodistribution of iron deposition at different time points in the process of liver fibrosis induced by thioacetamide (TAA) in rats. To our best knowledge, this is the first study using confocal μ-XRF to analyze hepatic iron deposition in hepatic fibrosis. The results showed that there are minor and trace elements such as iron, potassium, and zinc in the liver of rats. Continuous injection of TAA solution resulted in increasing liver iron deposition over time. The intensity of iron deposition in liver tissue was also significantly reduced after bone mesenchymal stem cells (BMSCs) were injected. These findings indicated that confocal μ-XRF can be used as a nondestructive and quantitative method of evaluating hepatic iron deposition in hepatic fibrosis, and iron deposition may play an important role in the progression of hepatic fibrosis induced by TAA. |
format | Online Article Text |
id | pubmed-8811927 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-88119272022-02-04 Determination of Hepatic Iron Deposition in Drug-Induced Liver Fibrosis in Rats by Confocal Micro-XRF Spectrometry Xu, Qianqian Xia, Wenjing Zhou, Lazhen Zou, Zhengwei Li, Qiuxia Deng, Lijun Wu, Sha Wang, Tao Cui, Jingduo Liu, Zhiguo Sun, Tianxi Ye, Junsong Li, Fangzuo ACS Omega [Image: see text] Liver fibrosis is the intermediate process and inevitable stage of the development of chronic liver disease into cirrhosis. Reducing the degree of liver fibrosis plays an extremely important role in treating chronic liver disease and preventing liver cirrhosis and liver cancer. The formation of liver fibrosis is affected by iron deposition to a certain extent, and excessive iron deposition further induces liver cirrhosis and liver cancer. Herein, confocal microbeam X-ray fluorescence (μ-XRF) was used to determine the intensity and biodistribution of iron deposition at different time points in the process of liver fibrosis induced by thioacetamide (TAA) in rats. To our best knowledge, this is the first study using confocal μ-XRF to analyze hepatic iron deposition in hepatic fibrosis. The results showed that there are minor and trace elements such as iron, potassium, and zinc in the liver of rats. Continuous injection of TAA solution resulted in increasing liver iron deposition over time. The intensity of iron deposition in liver tissue was also significantly reduced after bone mesenchymal stem cells (BMSCs) were injected. These findings indicated that confocal μ-XRF can be used as a nondestructive and quantitative method of evaluating hepatic iron deposition in hepatic fibrosis, and iron deposition may play an important role in the progression of hepatic fibrosis induced by TAA. American Chemical Society 2022-01-24 /pmc/articles/PMC8811927/ /pubmed/35128282 http://dx.doi.org/10.1021/acsomega.1c06476 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Xu, Qianqian Xia, Wenjing Zhou, Lazhen Zou, Zhengwei Li, Qiuxia Deng, Lijun Wu, Sha Wang, Tao Cui, Jingduo Liu, Zhiguo Sun, Tianxi Ye, Junsong Li, Fangzuo Determination of Hepatic Iron Deposition in Drug-Induced Liver Fibrosis in Rats by Confocal Micro-XRF Spectrometry |
title | Determination of Hepatic Iron Deposition in Drug-Induced
Liver Fibrosis in Rats by Confocal Micro-XRF Spectrometry |
title_full | Determination of Hepatic Iron Deposition in Drug-Induced
Liver Fibrosis in Rats by Confocal Micro-XRF Spectrometry |
title_fullStr | Determination of Hepatic Iron Deposition in Drug-Induced
Liver Fibrosis in Rats by Confocal Micro-XRF Spectrometry |
title_full_unstemmed | Determination of Hepatic Iron Deposition in Drug-Induced
Liver Fibrosis in Rats by Confocal Micro-XRF Spectrometry |
title_short | Determination of Hepatic Iron Deposition in Drug-Induced
Liver Fibrosis in Rats by Confocal Micro-XRF Spectrometry |
title_sort | determination of hepatic iron deposition in drug-induced
liver fibrosis in rats by confocal micro-xrf spectrometry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8811927/ https://www.ncbi.nlm.nih.gov/pubmed/35128282 http://dx.doi.org/10.1021/acsomega.1c06476 |
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