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IRF-2 inhibits cancer proliferation by promoting AMER-1 transcription in human gastric cancer
BACKGROUND: Interferon regulatory factor 2 (IRF-2) acts as an anti-oncogene in gastric cancer (GC); however, the underlying mechanism remains unknown. METHODS: This study determined the expression of IRF-2 in GC tissues and adjacent non-tumor tissues using immunohistochemistry (IHC) and explored the...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8812234/ https://www.ncbi.nlm.nih.gov/pubmed/35115027 http://dx.doi.org/10.1186/s12967-022-03275-0 |
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author | Chen, Yan-Jie Luo, Shu-Neng Wu, Hao Zhang, Ning-Ping Dong, Ling Liu, Tao-Tao Liang, Li Shen, Xi-Zhong |
author_facet | Chen, Yan-Jie Luo, Shu-Neng Wu, Hao Zhang, Ning-Ping Dong, Ling Liu, Tao-Tao Liang, Li Shen, Xi-Zhong |
author_sort | Chen, Yan-Jie |
collection | PubMed |
description | BACKGROUND: Interferon regulatory factor 2 (IRF-2) acts as an anti-oncogene in gastric cancer (GC); however, the underlying mechanism remains unknown. METHODS: This study determined the expression of IRF-2 in GC tissues and adjacent non-tumor tissues using immunohistochemistry (IHC) and explored the predictive value of IRF-2 for the prognoses of GC patients. Cell function and xenograft tumor growth experiments in nude mice were performed to test tumor proliferation ability, both in vitro and in vivo. Chromatin immunoprecipitation sequencing (ChIP-Seq) assay was used to verify the direct target of IRF-2. RESULTS: We found that IRF-2 expression was downregulated in GC tissues and was negatively correlated with the prognoses of GC patients. IRF-2 negatively affected GC cell proliferation both in vitro and in vivo. ChIP-Seq assay showed that IRF-2 could directly activate AMER-1 transcription and regulate the Wnt/β-catenin signaling pathway, which was validated using IHC, in both tissue microarray and xenografted tumor tissues, western blot analysis, and cell function experiments. CONCLUSIONS: Increased expression of IRF-2 can inhibit tumor growth and affect the prognoses of patients by directly regulating AMER-1 transcription in GC and inhibiting the Wnt/β-catenin signaling pathway. |
format | Online Article Text |
id | pubmed-8812234 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-88122342022-02-07 IRF-2 inhibits cancer proliferation by promoting AMER-1 transcription in human gastric cancer Chen, Yan-Jie Luo, Shu-Neng Wu, Hao Zhang, Ning-Ping Dong, Ling Liu, Tao-Tao Liang, Li Shen, Xi-Zhong J Transl Med Research BACKGROUND: Interferon regulatory factor 2 (IRF-2) acts as an anti-oncogene in gastric cancer (GC); however, the underlying mechanism remains unknown. METHODS: This study determined the expression of IRF-2 in GC tissues and adjacent non-tumor tissues using immunohistochemistry (IHC) and explored the predictive value of IRF-2 for the prognoses of GC patients. Cell function and xenograft tumor growth experiments in nude mice were performed to test tumor proliferation ability, both in vitro and in vivo. Chromatin immunoprecipitation sequencing (ChIP-Seq) assay was used to verify the direct target of IRF-2. RESULTS: We found that IRF-2 expression was downregulated in GC tissues and was negatively correlated with the prognoses of GC patients. IRF-2 negatively affected GC cell proliferation both in vitro and in vivo. ChIP-Seq assay showed that IRF-2 could directly activate AMER-1 transcription and regulate the Wnt/β-catenin signaling pathway, which was validated using IHC, in both tissue microarray and xenografted tumor tissues, western blot analysis, and cell function experiments. CONCLUSIONS: Increased expression of IRF-2 can inhibit tumor growth and affect the prognoses of patients by directly regulating AMER-1 transcription in GC and inhibiting the Wnt/β-catenin signaling pathway. BioMed Central 2022-02-03 /pmc/articles/PMC8812234/ /pubmed/35115027 http://dx.doi.org/10.1186/s12967-022-03275-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Chen, Yan-Jie Luo, Shu-Neng Wu, Hao Zhang, Ning-Ping Dong, Ling Liu, Tao-Tao Liang, Li Shen, Xi-Zhong IRF-2 inhibits cancer proliferation by promoting AMER-1 transcription in human gastric cancer |
title | IRF-2 inhibits cancer proliferation by promoting AMER-1 transcription in human gastric cancer |
title_full | IRF-2 inhibits cancer proliferation by promoting AMER-1 transcription in human gastric cancer |
title_fullStr | IRF-2 inhibits cancer proliferation by promoting AMER-1 transcription in human gastric cancer |
title_full_unstemmed | IRF-2 inhibits cancer proliferation by promoting AMER-1 transcription in human gastric cancer |
title_short | IRF-2 inhibits cancer proliferation by promoting AMER-1 transcription in human gastric cancer |
title_sort | irf-2 inhibits cancer proliferation by promoting amer-1 transcription in human gastric cancer |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8812234/ https://www.ncbi.nlm.nih.gov/pubmed/35115027 http://dx.doi.org/10.1186/s12967-022-03275-0 |
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