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Expression of DNM3 is associated with good outcome in colorectal cancer

The aim of this study is to reveal the potential value of dynamin3 (DNM3) in colorectal cancer (CRC) evaluation of clinical diagnosis and prognosis. A total of 100 tissue samples were collected from 50 patients with stages I–IV, CRC tissues (n = 50) paired with non-cancerous adjacent colorectal tiss...

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Autores principales: Cheng, Shao-ang, Huang, Xin, Jiang, Liang, Liang, Qi-Lian, Hong, Xiao-Cui, Yang, Hai-Xia, Hu, Ke-Hui, Luo, Xing-Bo, Zhang, Hui-Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: De Gruyter 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8812712/
https://www.ncbi.nlm.nih.gov/pubmed/35178475
http://dx.doi.org/10.1515/med-2022-0420
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author Cheng, Shao-ang
Huang, Xin
Jiang, Liang
Liang, Qi-Lian
Hong, Xiao-Cui
Yang, Hai-Xia
Hu, Ke-Hui
Luo, Xing-Bo
Zhang, Hui-Jie
author_facet Cheng, Shao-ang
Huang, Xin
Jiang, Liang
Liang, Qi-Lian
Hong, Xiao-Cui
Yang, Hai-Xia
Hu, Ke-Hui
Luo, Xing-Bo
Zhang, Hui-Jie
author_sort Cheng, Shao-ang
collection PubMed
description The aim of this study is to reveal the potential value of dynamin3 (DNM3) in colorectal cancer (CRC) evaluation of clinical diagnosis and prognosis. A total of 100 tissue samples were collected from 50 patients with stages I–IV, CRC tissues (n = 50) paired with non-cancerous adjacent colorectal tissues (n = 50). The expression levels of DNM3 were detected in 50 cases of CRC tissues and 50 cases of non-cancerous adjacent colorectal tissues by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR). Immunohistochemical method (IHC) was conducted to semi-quantify the expression of DNM3 protein. Results showed that the relative expression of DNM3 mRNA in CRC tissues was 0.634-fold of that in non-cancerous adjacent colorectal tissues. The positive rate of DNM3 protein in CRC tissues (42.0%) was much lower than that in non-cancerous adjacent colorectal tissues (66.0%; P < 0.05). The expression level of DNM3 protein in CRC tissues was dependent on tumor size, degree of histological differentiation, and clinical stage (P < 0.05). The expression level of DNM3 mRNA in CRC tissues was significantly correlated with tumor size and pathology classification (P < 0.05). The research shows that detecting the expression of DNM3 helps in analyzing the tumor size, degree of histological differentiation, and clinical stage. Expression of DNM3 may be associated with good outcome in CRC.
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spelling pubmed-88127122022-02-16 Expression of DNM3 is associated with good outcome in colorectal cancer Cheng, Shao-ang Huang, Xin Jiang, Liang Liang, Qi-Lian Hong, Xiao-Cui Yang, Hai-Xia Hu, Ke-Hui Luo, Xing-Bo Zhang, Hui-Jie Open Med (Wars) Research Article The aim of this study is to reveal the potential value of dynamin3 (DNM3) in colorectal cancer (CRC) evaluation of clinical diagnosis and prognosis. A total of 100 tissue samples were collected from 50 patients with stages I–IV, CRC tissues (n = 50) paired with non-cancerous adjacent colorectal tissues (n = 50). The expression levels of DNM3 were detected in 50 cases of CRC tissues and 50 cases of non-cancerous adjacent colorectal tissues by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR). Immunohistochemical method (IHC) was conducted to semi-quantify the expression of DNM3 protein. Results showed that the relative expression of DNM3 mRNA in CRC tissues was 0.634-fold of that in non-cancerous adjacent colorectal tissues. The positive rate of DNM3 protein in CRC tissues (42.0%) was much lower than that in non-cancerous adjacent colorectal tissues (66.0%; P < 0.05). The expression level of DNM3 protein in CRC tissues was dependent on tumor size, degree of histological differentiation, and clinical stage (P < 0.05). The expression level of DNM3 mRNA in CRC tissues was significantly correlated with tumor size and pathology classification (P < 0.05). The research shows that detecting the expression of DNM3 helps in analyzing the tumor size, degree of histological differentiation, and clinical stage. Expression of DNM3 may be associated with good outcome in CRC. De Gruyter 2022-01-24 /pmc/articles/PMC8812712/ /pubmed/35178475 http://dx.doi.org/10.1515/med-2022-0420 Text en © 2022 Shao-ang Cheng et al., published by De Gruyter https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License.
spellingShingle Research Article
Cheng, Shao-ang
Huang, Xin
Jiang, Liang
Liang, Qi-Lian
Hong, Xiao-Cui
Yang, Hai-Xia
Hu, Ke-Hui
Luo, Xing-Bo
Zhang, Hui-Jie
Expression of DNM3 is associated with good outcome in colorectal cancer
title Expression of DNM3 is associated with good outcome in colorectal cancer
title_full Expression of DNM3 is associated with good outcome in colorectal cancer
title_fullStr Expression of DNM3 is associated with good outcome in colorectal cancer
title_full_unstemmed Expression of DNM3 is associated with good outcome in colorectal cancer
title_short Expression of DNM3 is associated with good outcome in colorectal cancer
title_sort expression of dnm3 is associated with good outcome in colorectal cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8812712/
https://www.ncbi.nlm.nih.gov/pubmed/35178475
http://dx.doi.org/10.1515/med-2022-0420
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