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MicroRNA-143-3p/TBX3 Axis Represses Malignant Cell Behaviors in Bladder Cancer

OBJECTIVE: To offer new insight for bladder cancer therapy through researching the microRNA-143-3p/TBX3 axis. METHODS: Differentially expressed microRNAs in bladder cancer were provided by databases to find microRNA that may regulate TBX3. qRT-PCR was utilized to test levels of TBX3 mRNA and microRN...

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Detalles Bibliográficos
Autores principales: Huang, Lifu, Zhang, Xianjun, Li, Feiping, Wang, Xiaohong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8813229/
https://www.ncbi.nlm.nih.gov/pubmed/35126619
http://dx.doi.org/10.1155/2022/2880087
Descripción
Sumario:OBJECTIVE: To offer new insight for bladder cancer therapy through researching the microRNA-143-3p/TBX3 axis. METHODS: Differentially expressed microRNAs in bladder cancer were provided by databases to find microRNA that may regulate TBX3. qRT-PCR was utilized to test levels of TBX3 mRNA and microRNA-143-3p. Their binding was verified with a dual-luciferase method. Malignant cell behaviors were examined by cell functional experiments. Levels of TBX3 protein and proteins pertinent to epithelial-mesenchymal transition (EMT) were tested by western blot. RESULTS: TBX3 was highly expressed in bladder cancer cells. MicroRNA-143-3p presented the most conspicuously negative correlation with TBX3, and they had binding sites. Cell functional experiments proved that TBX3 facilitated bladder cancer cell functions and EMT. MicroRNA-143-3p was demonstrated to downregulate TBX3 expression. Rescue assay further illuminated that microRNA-143-3p repressed bladder cancer cell functions and EMT through downregulating TBX3 expression. CONCLUSION: These data all indicated that TBX3 was modulated by microRNA-143-3p and acted as a cancer promoter gene in bladder cancer progression via affecting tumor proliferation, migration, invasion, and EMT. Therefore, a microRNA-143-3p/TBX3 network might be an underlying target for bladder cancer.