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MicroRNA-143-3p/TBX3 Axis Represses Malignant Cell Behaviors in Bladder Cancer
OBJECTIVE: To offer new insight for bladder cancer therapy through researching the microRNA-143-3p/TBX3 axis. METHODS: Differentially expressed microRNAs in bladder cancer were provided by databases to find microRNA that may regulate TBX3. qRT-PCR was utilized to test levels of TBX3 mRNA and microRN...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8813229/ https://www.ncbi.nlm.nih.gov/pubmed/35126619 http://dx.doi.org/10.1155/2022/2880087 |
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author | Huang, Lifu Zhang, Xianjun Li, Feiping Wang, Xiaohong |
author_facet | Huang, Lifu Zhang, Xianjun Li, Feiping Wang, Xiaohong |
author_sort | Huang, Lifu |
collection | PubMed |
description | OBJECTIVE: To offer new insight for bladder cancer therapy through researching the microRNA-143-3p/TBX3 axis. METHODS: Differentially expressed microRNAs in bladder cancer were provided by databases to find microRNA that may regulate TBX3. qRT-PCR was utilized to test levels of TBX3 mRNA and microRNA-143-3p. Their binding was verified with a dual-luciferase method. Malignant cell behaviors were examined by cell functional experiments. Levels of TBX3 protein and proteins pertinent to epithelial-mesenchymal transition (EMT) were tested by western blot. RESULTS: TBX3 was highly expressed in bladder cancer cells. MicroRNA-143-3p presented the most conspicuously negative correlation with TBX3, and they had binding sites. Cell functional experiments proved that TBX3 facilitated bladder cancer cell functions and EMT. MicroRNA-143-3p was demonstrated to downregulate TBX3 expression. Rescue assay further illuminated that microRNA-143-3p repressed bladder cancer cell functions and EMT through downregulating TBX3 expression. CONCLUSION: These data all indicated that TBX3 was modulated by microRNA-143-3p and acted as a cancer promoter gene in bladder cancer progression via affecting tumor proliferation, migration, invasion, and EMT. Therefore, a microRNA-143-3p/TBX3 network might be an underlying target for bladder cancer. |
format | Online Article Text |
id | pubmed-8813229 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-88132292022-02-04 MicroRNA-143-3p/TBX3 Axis Represses Malignant Cell Behaviors in Bladder Cancer Huang, Lifu Zhang, Xianjun Li, Feiping Wang, Xiaohong Comput Math Methods Med Research Article OBJECTIVE: To offer new insight for bladder cancer therapy through researching the microRNA-143-3p/TBX3 axis. METHODS: Differentially expressed microRNAs in bladder cancer were provided by databases to find microRNA that may regulate TBX3. qRT-PCR was utilized to test levels of TBX3 mRNA and microRNA-143-3p. Their binding was verified with a dual-luciferase method. Malignant cell behaviors were examined by cell functional experiments. Levels of TBX3 protein and proteins pertinent to epithelial-mesenchymal transition (EMT) were tested by western blot. RESULTS: TBX3 was highly expressed in bladder cancer cells. MicroRNA-143-3p presented the most conspicuously negative correlation with TBX3, and they had binding sites. Cell functional experiments proved that TBX3 facilitated bladder cancer cell functions and EMT. MicroRNA-143-3p was demonstrated to downregulate TBX3 expression. Rescue assay further illuminated that microRNA-143-3p repressed bladder cancer cell functions and EMT through downregulating TBX3 expression. CONCLUSION: These data all indicated that TBX3 was modulated by microRNA-143-3p and acted as a cancer promoter gene in bladder cancer progression via affecting tumor proliferation, migration, invasion, and EMT. Therefore, a microRNA-143-3p/TBX3 network might be an underlying target for bladder cancer. Hindawi 2022-01-27 /pmc/articles/PMC8813229/ /pubmed/35126619 http://dx.doi.org/10.1155/2022/2880087 Text en Copyright © 2022 Lifu Huang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Huang, Lifu Zhang, Xianjun Li, Feiping Wang, Xiaohong MicroRNA-143-3p/TBX3 Axis Represses Malignant Cell Behaviors in Bladder Cancer |
title | MicroRNA-143-3p/TBX3 Axis Represses Malignant Cell Behaviors in Bladder Cancer |
title_full | MicroRNA-143-3p/TBX3 Axis Represses Malignant Cell Behaviors in Bladder Cancer |
title_fullStr | MicroRNA-143-3p/TBX3 Axis Represses Malignant Cell Behaviors in Bladder Cancer |
title_full_unstemmed | MicroRNA-143-3p/TBX3 Axis Represses Malignant Cell Behaviors in Bladder Cancer |
title_short | MicroRNA-143-3p/TBX3 Axis Represses Malignant Cell Behaviors in Bladder Cancer |
title_sort | microrna-143-3p/tbx3 axis represses malignant cell behaviors in bladder cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8813229/ https://www.ncbi.nlm.nih.gov/pubmed/35126619 http://dx.doi.org/10.1155/2022/2880087 |
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