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Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis

BACKGROUND: Traditional interventions can play a certain role in attenuating ulcerative colitis (UC), known as one type of inflammatory bowel diseases, but sometimes are not effective. Endometrial regenerative cells (ERCs) have been shown to exert immunosuppressive effects in different models of inf...

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Autores principales: Sun, Chenglu, Hao, Jingpeng, Qin, Hong, Zhu, Yanglin, Li, Xiang, Zhang, Baoren, Qin, Yafei, Li, Guangming, Wang, Hongda, Wang, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8813287/
https://www.ncbi.nlm.nih.gov/pubmed/35126527
http://dx.doi.org/10.1155/2022/7842296
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author Sun, Chenglu
Hao, Jingpeng
Qin, Hong
Zhu, Yanglin
Li, Xiang
Zhang, Baoren
Qin, Yafei
Li, Guangming
Wang, Hongda
Wang, Hao
author_facet Sun, Chenglu
Hao, Jingpeng
Qin, Hong
Zhu, Yanglin
Li, Xiang
Zhang, Baoren
Qin, Yafei
Li, Guangming
Wang, Hongda
Wang, Hao
author_sort Sun, Chenglu
collection PubMed
description BACKGROUND: Traditional interventions can play a certain role in attenuating ulcerative colitis (UC), known as one type of inflammatory bowel diseases, but sometimes are not effective. Endometrial regenerative cells (ERCs) have been shown to exert immunosuppressive effects in different models of inflammation, and stem cell-derived conditioned media (CM) have advantages over cell therapy in terms of easy access and direct action. However, whether ERC-CM could alleviate colitis remains unclear and will be explored in this study. METHODS: Menstrual blood was collected from healthy female volunteers to obtain ERCs and ERC-CM. Acute colitis was induced by 3% dextran sodium sulfate (DSS), and ERC-CM was injected on days 4, 6, and 8, respectively, after induction. The disease activity index was calculated through the record of weight change, bleeding, and fecal viscosity during the treatment process. Histological features, macrophage and CD4(+) T cell in the spleen and colon, and cytokine profiles in the sera and colon were measured. In addition, an in vitro lymphocyte proliferation assay was measured by using a CCK-8 kit in this study. RESULTS: ERC-CM treatment significantly improved the symptoms and histological changes in colitis mice. ERC-CM increased the percentage of Tregs in the spleen and colon but decreased the percentages of M1 macrophages and Th1 and Th17 cells in the spleen and decreased the population of Th17 cells in the colon. In addition, ERC-CM treatment decreased the local expression of TNF-α, IL-6, and iNOS in the colon. Furthermore, ERC-CM increased the levels of anti-inflammatory cytokines IL-10 and IL-27 but decreased proinflammatory cytokines IL-6 and IL-17 in the sera. In addition, ERC-CM significantly inhibited ConA-induced mouse lymphocyte proliferation in vitro. CONCLUSION: The results suggest that ERC-CM can exert similar therapeutic effects as ERCs and could be explored for future application of cell-free therapy in the treatment of colitis.
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spelling pubmed-88132872022-02-04 Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis Sun, Chenglu Hao, Jingpeng Qin, Hong Zhu, Yanglin Li, Xiang Zhang, Baoren Qin, Yafei Li, Guangming Wang, Hongda Wang, Hao Stem Cells Int Research Article BACKGROUND: Traditional interventions can play a certain role in attenuating ulcerative colitis (UC), known as one type of inflammatory bowel diseases, but sometimes are not effective. Endometrial regenerative cells (ERCs) have been shown to exert immunosuppressive effects in different models of inflammation, and stem cell-derived conditioned media (CM) have advantages over cell therapy in terms of easy access and direct action. However, whether ERC-CM could alleviate colitis remains unclear and will be explored in this study. METHODS: Menstrual blood was collected from healthy female volunteers to obtain ERCs and ERC-CM. Acute colitis was induced by 3% dextran sodium sulfate (DSS), and ERC-CM was injected on days 4, 6, and 8, respectively, after induction. The disease activity index was calculated through the record of weight change, bleeding, and fecal viscosity during the treatment process. Histological features, macrophage and CD4(+) T cell in the spleen and colon, and cytokine profiles in the sera and colon were measured. In addition, an in vitro lymphocyte proliferation assay was measured by using a CCK-8 kit in this study. RESULTS: ERC-CM treatment significantly improved the symptoms and histological changes in colitis mice. ERC-CM increased the percentage of Tregs in the spleen and colon but decreased the percentages of M1 macrophages and Th1 and Th17 cells in the spleen and decreased the population of Th17 cells in the colon. In addition, ERC-CM treatment decreased the local expression of TNF-α, IL-6, and iNOS in the colon. Furthermore, ERC-CM increased the levels of anti-inflammatory cytokines IL-10 and IL-27 but decreased proinflammatory cytokines IL-6 and IL-17 in the sera. In addition, ERC-CM significantly inhibited ConA-induced mouse lymphocyte proliferation in vitro. CONCLUSION: The results suggest that ERC-CM can exert similar therapeutic effects as ERCs and could be explored for future application of cell-free therapy in the treatment of colitis. Hindawi 2022-01-27 /pmc/articles/PMC8813287/ /pubmed/35126527 http://dx.doi.org/10.1155/2022/7842296 Text en Copyright © 2022 Chenglu Sun et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sun, Chenglu
Hao, Jingpeng
Qin, Hong
Zhu, Yanglin
Li, Xiang
Zhang, Baoren
Qin, Yafei
Li, Guangming
Wang, Hongda
Wang, Hao
Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis
title Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis
title_full Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis
title_fullStr Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis
title_full_unstemmed Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis
title_short Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis
title_sort endometrial regenerative cell-derived conditioned medium alleviates experimental colitis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8813287/
https://www.ncbi.nlm.nih.gov/pubmed/35126527
http://dx.doi.org/10.1155/2022/7842296
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