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Protective Role of Microglia on Neuronal Survival after Exposure to Amyloid Beta

Alzheimer’s disease (AD) is the most common cause of neurodegeneration. It is characterized by deposits of amyloid beta (Aβ) plaques and impaired memory. Microglia are associated with AD. They are activated in the AD brain and AD models. However, the exact role of microglia has not been established....

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Detalles Bibliográficos
Autores principales: Lee, Sunjun, Choi, Won-Seok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Chonnam National University Medical School 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8813657/
https://www.ncbi.nlm.nih.gov/pubmed/35169554
http://dx.doi.org/10.4068/cmj.2022.58.1.13
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author Lee, Sunjun
Choi, Won-Seok
author_facet Lee, Sunjun
Choi, Won-Seok
author_sort Lee, Sunjun
collection PubMed
description Alzheimer’s disease (AD) is the most common cause of neurodegeneration. It is characterized by deposits of amyloid beta (Aβ) plaques and impaired memory. Microglia are associated with AD. They are activated in the AD brain and AD models. However, the exact role of microglia has not been established. We thus investigated the role of microglia in AD models using a primary culture and an ex-vivo assay. We showed that oligomerized Aβ is toxic to neurons in the primary culture. In the ex-vivo assay, a microglial cell line removed amyloid plaques in the brain of 5XFAD (AD model) mice. To verify if microglia can be protective for the neuron, we co-cultured neurons with primary microglia and treated them with Aβ. The loss of neurons, induced by amyloid toxicity, was attenuated by co-cultured microglia. Taken together, our data suggest that microglia promote neuronal survival by phagocytic clearance of Aβ in AD models.
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spelling pubmed-88136572022-02-14 Protective Role of Microglia on Neuronal Survival after Exposure to Amyloid Beta Lee, Sunjun Choi, Won-Seok Chonnam Med J Original Article Alzheimer’s disease (AD) is the most common cause of neurodegeneration. It is characterized by deposits of amyloid beta (Aβ) plaques and impaired memory. Microglia are associated with AD. They are activated in the AD brain and AD models. However, the exact role of microglia has not been established. We thus investigated the role of microglia in AD models using a primary culture and an ex-vivo assay. We showed that oligomerized Aβ is toxic to neurons in the primary culture. In the ex-vivo assay, a microglial cell line removed amyloid plaques in the brain of 5XFAD (AD model) mice. To verify if microglia can be protective for the neuron, we co-cultured neurons with primary microglia and treated them with Aβ. The loss of neurons, induced by amyloid toxicity, was attenuated by co-cultured microglia. Taken together, our data suggest that microglia promote neuronal survival by phagocytic clearance of Aβ in AD models. Chonnam National University Medical School 2022-01 2022-01-25 /pmc/articles/PMC8813657/ /pubmed/35169554 http://dx.doi.org/10.4068/cmj.2022.58.1.13 Text en © Chonnam Medical Journal, 2022 https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lee, Sunjun
Choi, Won-Seok
Protective Role of Microglia on Neuronal Survival after Exposure to Amyloid Beta
title Protective Role of Microglia on Neuronal Survival after Exposure to Amyloid Beta
title_full Protective Role of Microglia on Neuronal Survival after Exposure to Amyloid Beta
title_fullStr Protective Role of Microglia on Neuronal Survival after Exposure to Amyloid Beta
title_full_unstemmed Protective Role of Microglia on Neuronal Survival after Exposure to Amyloid Beta
title_short Protective Role of Microglia on Neuronal Survival after Exposure to Amyloid Beta
title_sort protective role of microglia on neuronal survival after exposure to amyloid beta
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8813657/
https://www.ncbi.nlm.nih.gov/pubmed/35169554
http://dx.doi.org/10.4068/cmj.2022.58.1.13
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