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Insights and genetic features of extended-spectrum beta-lactamase producing Escherichia coli isolates from two hospitals in Ghana

Recently, the emergence and rapid dissemination of extended-spectrum beta-lactamase (ESBL)-producing bacteria, particularly of the family Enterobacteriaceae, has posed serious healthcare challenges. Here, we determined the antimicrobial susceptibility and genetic characteristics of 164 Escherichia c...

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Autores principales: Mahazu, Samiratu, Sato, Wakana, Ayibieke, Alafate, Prah, Isaac, Hayashi, Takaya, Suzuki, Toshihiko, Iwanaga, Shiroh, Ablordey, Anthony, Saito, Ryoichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8813988/
https://www.ncbi.nlm.nih.gov/pubmed/35115628
http://dx.doi.org/10.1038/s41598-022-05869-6
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author Mahazu, Samiratu
Sato, Wakana
Ayibieke, Alafate
Prah, Isaac
Hayashi, Takaya
Suzuki, Toshihiko
Iwanaga, Shiroh
Ablordey, Anthony
Saito, Ryoichi
author_facet Mahazu, Samiratu
Sato, Wakana
Ayibieke, Alafate
Prah, Isaac
Hayashi, Takaya
Suzuki, Toshihiko
Iwanaga, Shiroh
Ablordey, Anthony
Saito, Ryoichi
author_sort Mahazu, Samiratu
collection PubMed
description Recently, the emergence and rapid dissemination of extended-spectrum beta-lactamase (ESBL)-producing bacteria, particularly of the family Enterobacteriaceae, has posed serious healthcare challenges. Here, we determined the antimicrobial susceptibility and genetic characteristics of 164 Escherichia coli strains isolated from infected patients in two hospitals in Ghana. In total, 102 cefotaxime-resistant isolates (62.2%) were identified as ESBL-producers. Multilocus sequence typing of the ESBL-producers identified 20 different sequence types (STs) with ST131 (n = 25, 24.5%) as the dominant group. Other detected STs included ST410 (n = 21, 20.6%) and ST617 (n = 19, 18.6%). All identified ESBL-producers harbored bla(CTX-M-14), bla(CTX-M-15), or bla(CTX-M-27), with bla(CTX-M-15) (n = 96, 94.1%) being the most predominant ESBL allele. Further analysis showed that the immediate genetic environment around bla(CTX-M-15) is conserved within bla(CTX-M-15) containing strains. Five of the 25 ST131 isolates were clustered with clade A, one with sub-clade C1, and 19 with the dominant sub-clade C2. The results show that fluoroquinolone-resistant, bla(CTX-M-14)- and bla(CTX- M-15)-producing ESBL E. coli ST131 strains belonging to clade A and sub-clades C1 and C2 are disseminating in Ghanaian hospitals. To the best of our knowledge, this is the first report of the ST131 phylogeny in Ghana.
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spelling pubmed-88139882022-02-07 Insights and genetic features of extended-spectrum beta-lactamase producing Escherichia coli isolates from two hospitals in Ghana Mahazu, Samiratu Sato, Wakana Ayibieke, Alafate Prah, Isaac Hayashi, Takaya Suzuki, Toshihiko Iwanaga, Shiroh Ablordey, Anthony Saito, Ryoichi Sci Rep Article Recently, the emergence and rapid dissemination of extended-spectrum beta-lactamase (ESBL)-producing bacteria, particularly of the family Enterobacteriaceae, has posed serious healthcare challenges. Here, we determined the antimicrobial susceptibility and genetic characteristics of 164 Escherichia coli strains isolated from infected patients in two hospitals in Ghana. In total, 102 cefotaxime-resistant isolates (62.2%) were identified as ESBL-producers. Multilocus sequence typing of the ESBL-producers identified 20 different sequence types (STs) with ST131 (n = 25, 24.5%) as the dominant group. Other detected STs included ST410 (n = 21, 20.6%) and ST617 (n = 19, 18.6%). All identified ESBL-producers harbored bla(CTX-M-14), bla(CTX-M-15), or bla(CTX-M-27), with bla(CTX-M-15) (n = 96, 94.1%) being the most predominant ESBL allele. Further analysis showed that the immediate genetic environment around bla(CTX-M-15) is conserved within bla(CTX-M-15) containing strains. Five of the 25 ST131 isolates were clustered with clade A, one with sub-clade C1, and 19 with the dominant sub-clade C2. The results show that fluoroquinolone-resistant, bla(CTX-M-14)- and bla(CTX- M-15)-producing ESBL E. coli ST131 strains belonging to clade A and sub-clades C1 and C2 are disseminating in Ghanaian hospitals. To the best of our knowledge, this is the first report of the ST131 phylogeny in Ghana. Nature Publishing Group UK 2022-02-03 /pmc/articles/PMC8813988/ /pubmed/35115628 http://dx.doi.org/10.1038/s41598-022-05869-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Mahazu, Samiratu
Sato, Wakana
Ayibieke, Alafate
Prah, Isaac
Hayashi, Takaya
Suzuki, Toshihiko
Iwanaga, Shiroh
Ablordey, Anthony
Saito, Ryoichi
Insights and genetic features of extended-spectrum beta-lactamase producing Escherichia coli isolates from two hospitals in Ghana
title Insights and genetic features of extended-spectrum beta-lactamase producing Escherichia coli isolates from two hospitals in Ghana
title_full Insights and genetic features of extended-spectrum beta-lactamase producing Escherichia coli isolates from two hospitals in Ghana
title_fullStr Insights and genetic features of extended-spectrum beta-lactamase producing Escherichia coli isolates from two hospitals in Ghana
title_full_unstemmed Insights and genetic features of extended-spectrum beta-lactamase producing Escherichia coli isolates from two hospitals in Ghana
title_short Insights and genetic features of extended-spectrum beta-lactamase producing Escherichia coli isolates from two hospitals in Ghana
title_sort insights and genetic features of extended-spectrum beta-lactamase producing escherichia coli isolates from two hospitals in ghana
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8813988/
https://www.ncbi.nlm.nih.gov/pubmed/35115628
http://dx.doi.org/10.1038/s41598-022-05869-6
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