Cargando…

Metal ion scavenging activity of elastin-like peptide analogues containing a cadmium ion binding sequence

The development of simple and safe methods for recovering environmental pollutants, such as heavy metals, is needed for sustainable environmental management. Short elastin-like peptide (ELP) analogues conjugated with metal chelating agents are considered to be useful as metal sequestering agents as...

Descripción completa

Detalles Bibliográficos
Autores principales: Sumiyoshi, Shogo, Suyama, Keitaro, Tatsubo, Daiki, Tanaka, Naoki, Tomohara, Keisuke, Taniguchi, Suguru, Maeda, Iori, Nose, Takeru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8814041/
https://www.ncbi.nlm.nih.gov/pubmed/35115613
http://dx.doi.org/10.1038/s41598-022-05695-w
_version_ 1784644990990811136
author Sumiyoshi, Shogo
Suyama, Keitaro
Tatsubo, Daiki
Tanaka, Naoki
Tomohara, Keisuke
Taniguchi, Suguru
Maeda, Iori
Nose, Takeru
author_facet Sumiyoshi, Shogo
Suyama, Keitaro
Tatsubo, Daiki
Tanaka, Naoki
Tomohara, Keisuke
Taniguchi, Suguru
Maeda, Iori
Nose, Takeru
author_sort Sumiyoshi, Shogo
collection PubMed
description The development of simple and safe methods for recovering environmental pollutants, such as heavy metals, is needed for sustainable environmental management. Short elastin-like peptide (ELP) analogues conjugated with metal chelating agents are considered to be useful as metal sequestering agents as they are readily produced, environment friendly, and the metal binding domain can be selected based on any target metal of interest. Due to the temperature dependent self-assembly of ELP, the peptide-based sequestering agents can be transformed from the solution state into the particles that chelate metal ions, which can then be collected as precipitates. In this study, we developed a peptide-based sequestering agent, AADAAC-(FPGVG)(4), by introducing the metal-binding sequence AADAAC on the N-terminus of a short ELP, (FPGVG)(4). In turbidity measurements, AADAAC-(FPGVG)(4) revealed strong self-assembling ability in the presence of metal ions such as Cd(2+) and Zn(2+). The results from colorimetric analysis indicated that AADAAC-(FPGVG)(4) could capture Cd(2+) and Zn(2+). Furthermore, AADAAC-(FPGVG)(4) that bound to metal ions could be readily recycled by treatment with acidic solution without compromising its metal binding affinity. The present study indicates that the fusion of the metal-binding sequence and ELP is a useful and powerful strategy to develop cost-effective heavy metal scavenging agents with low environmental impacts.
format Online
Article
Text
id pubmed-8814041
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-88140412022-02-07 Metal ion scavenging activity of elastin-like peptide analogues containing a cadmium ion binding sequence Sumiyoshi, Shogo Suyama, Keitaro Tatsubo, Daiki Tanaka, Naoki Tomohara, Keisuke Taniguchi, Suguru Maeda, Iori Nose, Takeru Sci Rep Article The development of simple and safe methods for recovering environmental pollutants, such as heavy metals, is needed for sustainable environmental management. Short elastin-like peptide (ELP) analogues conjugated with metal chelating agents are considered to be useful as metal sequestering agents as they are readily produced, environment friendly, and the metal binding domain can be selected based on any target metal of interest. Due to the temperature dependent self-assembly of ELP, the peptide-based sequestering agents can be transformed from the solution state into the particles that chelate metal ions, which can then be collected as precipitates. In this study, we developed a peptide-based sequestering agent, AADAAC-(FPGVG)(4), by introducing the metal-binding sequence AADAAC on the N-terminus of a short ELP, (FPGVG)(4). In turbidity measurements, AADAAC-(FPGVG)(4) revealed strong self-assembling ability in the presence of metal ions such as Cd(2+) and Zn(2+). The results from colorimetric analysis indicated that AADAAC-(FPGVG)(4) could capture Cd(2+) and Zn(2+). Furthermore, AADAAC-(FPGVG)(4) that bound to metal ions could be readily recycled by treatment with acidic solution without compromising its metal binding affinity. The present study indicates that the fusion of the metal-binding sequence and ELP is a useful and powerful strategy to develop cost-effective heavy metal scavenging agents with low environmental impacts. Nature Publishing Group UK 2022-02-03 /pmc/articles/PMC8814041/ /pubmed/35115613 http://dx.doi.org/10.1038/s41598-022-05695-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Sumiyoshi, Shogo
Suyama, Keitaro
Tatsubo, Daiki
Tanaka, Naoki
Tomohara, Keisuke
Taniguchi, Suguru
Maeda, Iori
Nose, Takeru
Metal ion scavenging activity of elastin-like peptide analogues containing a cadmium ion binding sequence
title Metal ion scavenging activity of elastin-like peptide analogues containing a cadmium ion binding sequence
title_full Metal ion scavenging activity of elastin-like peptide analogues containing a cadmium ion binding sequence
title_fullStr Metal ion scavenging activity of elastin-like peptide analogues containing a cadmium ion binding sequence
title_full_unstemmed Metal ion scavenging activity of elastin-like peptide analogues containing a cadmium ion binding sequence
title_short Metal ion scavenging activity of elastin-like peptide analogues containing a cadmium ion binding sequence
title_sort metal ion scavenging activity of elastin-like peptide analogues containing a cadmium ion binding sequence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8814041/
https://www.ncbi.nlm.nih.gov/pubmed/35115613
http://dx.doi.org/10.1038/s41598-022-05695-w
work_keys_str_mv AT sumiyoshishogo metalionscavengingactivityofelastinlikepeptideanaloguescontainingacadmiumionbindingsequence
AT suyamakeitaro metalionscavengingactivityofelastinlikepeptideanaloguescontainingacadmiumionbindingsequence
AT tatsubodaiki metalionscavengingactivityofelastinlikepeptideanaloguescontainingacadmiumionbindingsequence
AT tanakanaoki metalionscavengingactivityofelastinlikepeptideanaloguescontainingacadmiumionbindingsequence
AT tomoharakeisuke metalionscavengingactivityofelastinlikepeptideanaloguescontainingacadmiumionbindingsequence
AT taniguchisuguru metalionscavengingactivityofelastinlikepeptideanaloguescontainingacadmiumionbindingsequence
AT maedaiori metalionscavengingactivityofelastinlikepeptideanaloguescontainingacadmiumionbindingsequence
AT nosetakeru metalionscavengingactivityofelastinlikepeptideanaloguescontainingacadmiumionbindingsequence