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Comprehensive Analysis of Immune-Related Prognosis of TK1 in Hepatocellular Carcinoma

Increased expression of TK1 is associated with the progression of a variety of tumors. However, the relationship of TK1 expression with immune cell infiltration and its prognostic value in hepatocellular carcinoma (HCC) are still unknown. In this study the TCGA database was used to evaluate TK1 expr...

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Autores principales: Cai, Qun, Zhu, Mingyan, Duan, Jinnan, Wang, Hao, Chen, Jingdan, Xiao, Yixin, Wang, Yangqin, Wang, Jianfang, Yu, Xuewen, Yang, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8814100/
https://www.ncbi.nlm.nih.gov/pubmed/35127491
http://dx.doi.org/10.3389/fonc.2021.786873
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author Cai, Qun
Zhu, Mingyan
Duan, Jinnan
Wang, Hao
Chen, Jingdan
Xiao, Yixin
Wang, Yangqin
Wang, Jianfang
Yu, Xuewen
Yang, Hui
author_facet Cai, Qun
Zhu, Mingyan
Duan, Jinnan
Wang, Hao
Chen, Jingdan
Xiao, Yixin
Wang, Yangqin
Wang, Jianfang
Yu, Xuewen
Yang, Hui
author_sort Cai, Qun
collection PubMed
description Increased expression of TK1 is associated with the progression of a variety of tumors. However, the relationship of TK1 expression with immune cell infiltration and its prognostic value in hepatocellular carcinoma (HCC) are still unknown. In this study the TCGA database was used to evaluate TK1 expression and its impact on survival in patients with HCC. Compared with normal tissue, TK1 in the liver tissue of patients with HCC was significantly up-regulated at both the mRNA and protein levels. Furthermore, TK1 expression was significantly related to pathological stage, tumor stage and lymph node metastasis, with high TK1 expression being an unfavorable prognostic factor for HCC. TK1 expression was also significantly associated with the infiltration of B cells, T cells, and dendritic cells in HCC. Single-cell sequencing analysis revealed that TK1 was associated with relatively large changes in T cells, especially gamma-delta T cells. A prognostic risk score based on TK1-related immune genes (CD40LG and TNFRSF4) was established using COX regression analysis. By integrating the immune-related risk score model with clinical features, a nomogram was constructed to predict the survival rate of HCC patients (1 year, 3-year and 5-year AUC of 0.782, 0.783 and 0.771, respectively). Knockdown of the target gene for TK1 was found to have significant anti-apoptosis and pro-proliferation effects on HepG2 cells. The level of TK1 in the serum and liver tissue of patients with HCC was significantly increased relative to healthy controls. These findings highlight the role of TK1 in the tumor immune response of HCC patients and in the proliferation and apoptosis of HepG2 cells. TK1 could therefore be a potential immunotherapy target for HCC patients, while the two immune genes related to TK1 (CD40LG And TNFRSF4) may be promising prognostic biomarkers in HCC.
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spelling pubmed-88141002022-02-05 Comprehensive Analysis of Immune-Related Prognosis of TK1 in Hepatocellular Carcinoma Cai, Qun Zhu, Mingyan Duan, Jinnan Wang, Hao Chen, Jingdan Xiao, Yixin Wang, Yangqin Wang, Jianfang Yu, Xuewen Yang, Hui Front Oncol Oncology Increased expression of TK1 is associated with the progression of a variety of tumors. However, the relationship of TK1 expression with immune cell infiltration and its prognostic value in hepatocellular carcinoma (HCC) are still unknown. In this study the TCGA database was used to evaluate TK1 expression and its impact on survival in patients with HCC. Compared with normal tissue, TK1 in the liver tissue of patients with HCC was significantly up-regulated at both the mRNA and protein levels. Furthermore, TK1 expression was significantly related to pathological stage, tumor stage and lymph node metastasis, with high TK1 expression being an unfavorable prognostic factor for HCC. TK1 expression was also significantly associated with the infiltration of B cells, T cells, and dendritic cells in HCC. Single-cell sequencing analysis revealed that TK1 was associated with relatively large changes in T cells, especially gamma-delta T cells. A prognostic risk score based on TK1-related immune genes (CD40LG and TNFRSF4) was established using COX regression analysis. By integrating the immune-related risk score model with clinical features, a nomogram was constructed to predict the survival rate of HCC patients (1 year, 3-year and 5-year AUC of 0.782, 0.783 and 0.771, respectively). Knockdown of the target gene for TK1 was found to have significant anti-apoptosis and pro-proliferation effects on HepG2 cells. The level of TK1 in the serum and liver tissue of patients with HCC was significantly increased relative to healthy controls. These findings highlight the role of TK1 in the tumor immune response of HCC patients and in the proliferation and apoptosis of HepG2 cells. TK1 could therefore be a potential immunotherapy target for HCC patients, while the two immune genes related to TK1 (CD40LG And TNFRSF4) may be promising prognostic biomarkers in HCC. Frontiers Media S.A. 2022-01-21 /pmc/articles/PMC8814100/ /pubmed/35127491 http://dx.doi.org/10.3389/fonc.2021.786873 Text en Copyright © 2022 Cai, Zhu, Duan, Wang, Chen, Xiao, Wang, Wang, Yu and Yang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Cai, Qun
Zhu, Mingyan
Duan, Jinnan
Wang, Hao
Chen, Jingdan
Xiao, Yixin
Wang, Yangqin
Wang, Jianfang
Yu, Xuewen
Yang, Hui
Comprehensive Analysis of Immune-Related Prognosis of TK1 in Hepatocellular Carcinoma
title Comprehensive Analysis of Immune-Related Prognosis of TK1 in Hepatocellular Carcinoma
title_full Comprehensive Analysis of Immune-Related Prognosis of TK1 in Hepatocellular Carcinoma
title_fullStr Comprehensive Analysis of Immune-Related Prognosis of TK1 in Hepatocellular Carcinoma
title_full_unstemmed Comprehensive Analysis of Immune-Related Prognosis of TK1 in Hepatocellular Carcinoma
title_short Comprehensive Analysis of Immune-Related Prognosis of TK1 in Hepatocellular Carcinoma
title_sort comprehensive analysis of immune-related prognosis of tk1 in hepatocellular carcinoma
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8814100/
https://www.ncbi.nlm.nih.gov/pubmed/35127491
http://dx.doi.org/10.3389/fonc.2021.786873
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