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Proteomic landscape of SARS-CoV-2– and MERS-CoV–infected primary human renal epithelial cells
Acute kidney injury is associated with mortality in COVID-19 patients. However, host cell changes underlying infection of renal cells with SARS-CoV-2 remain unknown and prevent understanding of the molecular mechanisms that may contribute to renal pathology. Here, we carried out quantitative transla...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Life Science Alliance LLC
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8814637/ https://www.ncbi.nlm.nih.gov/pubmed/35110370 http://dx.doi.org/10.26508/lsa.202201371 |
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author | Kohli, Aneesha Sauerhering, Lucie Fehling, Sarah K Klann, Kevin Geiger, Helmut Becker, Stephan Koch, Benjamin Baer, Patrick C Strecker, Thomas Münch, Christian |
author_facet | Kohli, Aneesha Sauerhering, Lucie Fehling, Sarah K Klann, Kevin Geiger, Helmut Becker, Stephan Koch, Benjamin Baer, Patrick C Strecker, Thomas Münch, Christian |
author_sort | Kohli, Aneesha |
collection | PubMed |
description | Acute kidney injury is associated with mortality in COVID-19 patients. However, host cell changes underlying infection of renal cells with SARS-CoV-2 remain unknown and prevent understanding of the molecular mechanisms that may contribute to renal pathology. Here, we carried out quantitative translatome and whole-cell proteomics analyses of primary renal proximal and distal tubular epithelial cells derived from human donors infected with SARS-CoV-2 or MERS-CoV to disseminate virus and cell type–specific changes over time. Our findings revealed shared pathways modified upon infection with both viruses, as well as SARS-CoV-2-specific host cell modulation driving key changes in innate immune activation and cellular protein quality control. Notably, MERS-CoV infection–induced specific changes in mitochondrial biology that were not observed in response to SARS-CoV-2 infection. Furthermore, we identified extensive modulation in pathways associated with kidney failure that changed in a virus- and cell type–specific manner. In summary, we provide an overview of the effects of SARS-CoV-2 or MERS-CoV infection on primary renal epithelial cells revealing key pathways that may be essential for viral replication. |
format | Online Article Text |
id | pubmed-8814637 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Life Science Alliance LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-88146372022-02-16 Proteomic landscape of SARS-CoV-2– and MERS-CoV–infected primary human renal epithelial cells Kohli, Aneesha Sauerhering, Lucie Fehling, Sarah K Klann, Kevin Geiger, Helmut Becker, Stephan Koch, Benjamin Baer, Patrick C Strecker, Thomas Münch, Christian Life Sci Alliance Research Articles Acute kidney injury is associated with mortality in COVID-19 patients. However, host cell changes underlying infection of renal cells with SARS-CoV-2 remain unknown and prevent understanding of the molecular mechanisms that may contribute to renal pathology. Here, we carried out quantitative translatome and whole-cell proteomics analyses of primary renal proximal and distal tubular epithelial cells derived from human donors infected with SARS-CoV-2 or MERS-CoV to disseminate virus and cell type–specific changes over time. Our findings revealed shared pathways modified upon infection with both viruses, as well as SARS-CoV-2-specific host cell modulation driving key changes in innate immune activation and cellular protein quality control. Notably, MERS-CoV infection–induced specific changes in mitochondrial biology that were not observed in response to SARS-CoV-2 infection. Furthermore, we identified extensive modulation in pathways associated with kidney failure that changed in a virus- and cell type–specific manner. In summary, we provide an overview of the effects of SARS-CoV-2 or MERS-CoV infection on primary renal epithelial cells revealing key pathways that may be essential for viral replication. Life Science Alliance LLC 2022-02-02 /pmc/articles/PMC8814637/ /pubmed/35110370 http://dx.doi.org/10.26508/lsa.202201371 Text en © 2022 Kohli et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Articles Kohli, Aneesha Sauerhering, Lucie Fehling, Sarah K Klann, Kevin Geiger, Helmut Becker, Stephan Koch, Benjamin Baer, Patrick C Strecker, Thomas Münch, Christian Proteomic landscape of SARS-CoV-2– and MERS-CoV–infected primary human renal epithelial cells |
title | Proteomic landscape of SARS-CoV-2– and MERS-CoV–infected primary human renal epithelial cells |
title_full | Proteomic landscape of SARS-CoV-2– and MERS-CoV–infected primary human renal epithelial cells |
title_fullStr | Proteomic landscape of SARS-CoV-2– and MERS-CoV–infected primary human renal epithelial cells |
title_full_unstemmed | Proteomic landscape of SARS-CoV-2– and MERS-CoV–infected primary human renal epithelial cells |
title_short | Proteomic landscape of SARS-CoV-2– and MERS-CoV–infected primary human renal epithelial cells |
title_sort | proteomic landscape of sars-cov-2– and mers-cov–infected primary human renal epithelial cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8814637/ https://www.ncbi.nlm.nih.gov/pubmed/35110370 http://dx.doi.org/10.26508/lsa.202201371 |
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