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Proteomic landscape of SARS-CoV-2– and MERS-CoV–infected primary human renal epithelial cells

Acute kidney injury is associated with mortality in COVID-19 patients. However, host cell changes underlying infection of renal cells with SARS-CoV-2 remain unknown and prevent understanding of the molecular mechanisms that may contribute to renal pathology. Here, we carried out quantitative transla...

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Autores principales: Kohli, Aneesha, Sauerhering, Lucie, Fehling, Sarah K, Klann, Kevin, Geiger, Helmut, Becker, Stephan, Koch, Benjamin, Baer, Patrick C, Strecker, Thomas, Münch, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8814637/
https://www.ncbi.nlm.nih.gov/pubmed/35110370
http://dx.doi.org/10.26508/lsa.202201371
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author Kohli, Aneesha
Sauerhering, Lucie
Fehling, Sarah K
Klann, Kevin
Geiger, Helmut
Becker, Stephan
Koch, Benjamin
Baer, Patrick C
Strecker, Thomas
Münch, Christian
author_facet Kohli, Aneesha
Sauerhering, Lucie
Fehling, Sarah K
Klann, Kevin
Geiger, Helmut
Becker, Stephan
Koch, Benjamin
Baer, Patrick C
Strecker, Thomas
Münch, Christian
author_sort Kohli, Aneesha
collection PubMed
description Acute kidney injury is associated with mortality in COVID-19 patients. However, host cell changes underlying infection of renal cells with SARS-CoV-2 remain unknown and prevent understanding of the molecular mechanisms that may contribute to renal pathology. Here, we carried out quantitative translatome and whole-cell proteomics analyses of primary renal proximal and distal tubular epithelial cells derived from human donors infected with SARS-CoV-2 or MERS-CoV to disseminate virus and cell type–specific changes over time. Our findings revealed shared pathways modified upon infection with both viruses, as well as SARS-CoV-2-specific host cell modulation driving key changes in innate immune activation and cellular protein quality control. Notably, MERS-CoV infection–induced specific changes in mitochondrial biology that were not observed in response to SARS-CoV-2 infection. Furthermore, we identified extensive modulation in pathways associated with kidney failure that changed in a virus- and cell type–specific manner. In summary, we provide an overview of the effects of SARS-CoV-2 or MERS-CoV infection on primary renal epithelial cells revealing key pathways that may be essential for viral replication.
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spelling pubmed-88146372022-02-16 Proteomic landscape of SARS-CoV-2– and MERS-CoV–infected primary human renal epithelial cells Kohli, Aneesha Sauerhering, Lucie Fehling, Sarah K Klann, Kevin Geiger, Helmut Becker, Stephan Koch, Benjamin Baer, Patrick C Strecker, Thomas Münch, Christian Life Sci Alliance Research Articles Acute kidney injury is associated with mortality in COVID-19 patients. However, host cell changes underlying infection of renal cells with SARS-CoV-2 remain unknown and prevent understanding of the molecular mechanisms that may contribute to renal pathology. Here, we carried out quantitative translatome and whole-cell proteomics analyses of primary renal proximal and distal tubular epithelial cells derived from human donors infected with SARS-CoV-2 or MERS-CoV to disseminate virus and cell type–specific changes over time. Our findings revealed shared pathways modified upon infection with both viruses, as well as SARS-CoV-2-specific host cell modulation driving key changes in innate immune activation and cellular protein quality control. Notably, MERS-CoV infection–induced specific changes in mitochondrial biology that were not observed in response to SARS-CoV-2 infection. Furthermore, we identified extensive modulation in pathways associated with kidney failure that changed in a virus- and cell type–specific manner. In summary, we provide an overview of the effects of SARS-CoV-2 or MERS-CoV infection on primary renal epithelial cells revealing key pathways that may be essential for viral replication. Life Science Alliance LLC 2022-02-02 /pmc/articles/PMC8814637/ /pubmed/35110370 http://dx.doi.org/10.26508/lsa.202201371 Text en © 2022 Kohli et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Articles
Kohli, Aneesha
Sauerhering, Lucie
Fehling, Sarah K
Klann, Kevin
Geiger, Helmut
Becker, Stephan
Koch, Benjamin
Baer, Patrick C
Strecker, Thomas
Münch, Christian
Proteomic landscape of SARS-CoV-2– and MERS-CoV–infected primary human renal epithelial cells
title Proteomic landscape of SARS-CoV-2– and MERS-CoV–infected primary human renal epithelial cells
title_full Proteomic landscape of SARS-CoV-2– and MERS-CoV–infected primary human renal epithelial cells
title_fullStr Proteomic landscape of SARS-CoV-2– and MERS-CoV–infected primary human renal epithelial cells
title_full_unstemmed Proteomic landscape of SARS-CoV-2– and MERS-CoV–infected primary human renal epithelial cells
title_short Proteomic landscape of SARS-CoV-2– and MERS-CoV–infected primary human renal epithelial cells
title_sort proteomic landscape of sars-cov-2– and mers-cov–infected primary human renal epithelial cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8814637/
https://www.ncbi.nlm.nih.gov/pubmed/35110370
http://dx.doi.org/10.26508/lsa.202201371
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