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Protocol to assess the effects of dysfunctional human vascular smooth muscle cells on other brain cells using in vitro models of Alzheimer’s disease

Here, we present a protocol to culture primary human vascular smooth muscle cells (VSMCs) under Alzheimer's disease (AD)-like conditions, including steps for morphological characterization with microscopy. We then describe functional assays, including wound healing, transwell, coculture, and su...

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Detalles Bibliográficos
Autores principales:	Alvarez, Karla Lucia F., Aguilar-Pineda, Jorge, Vera-Lopez, Karin J., Lino Cardenas, Christian L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8814650/ https://www.ncbi.nlm.nih.gov/pubmed/35141568 http://dx.doi.org/10.1016/j.xpro.2022.101149

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