Cargando…

The Intrinsic Blue Light Responses of Avian Müller Glial Cells Imply Calcium Release from Internal Stores

The retina of vertebrates is responsible for capturing light through visual (cones and rods) and non-visual photoreceptors (intrinsically photosensitive retinal ganglion cells and horizontal cells) triggering a number of essential activities associated to image- and non-image forming functions (phot...

Descripción completa

Detalles Bibliográficos
Autores principales: Marchese, Natalia A., Ríos, Maximiliano N., Guido, Mario E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8814826/
https://www.ncbi.nlm.nih.gov/pubmed/35103506
http://dx.doi.org/10.1177/17590914221076698
_version_ 1784645149088808960
author Marchese, Natalia A.
Ríos, Maximiliano N.
Guido, Mario E.
author_facet Marchese, Natalia A.
Ríos, Maximiliano N.
Guido, Mario E.
author_sort Marchese, Natalia A.
collection PubMed
description The retina of vertebrates is responsible for capturing light through visual (cones and rods) and non-visual photoreceptors (intrinsically photosensitive retinal ganglion cells and horizontal cells) triggering a number of essential activities associated to image- and non-image forming functions (photic entrainment of daily rhythms, pupillary light reflexes, pineal melatonin inhibition, among others). Although the retina contains diverse types of neuronal based-photoreceptors cells, originally classified as ciliary- or rhabdomeric-like types, in recent years, it has been shown that the major glial cell type of the retina, the Müller glial cells (MC), express blue photopigments as Opn3 (encephalopsin) and Opn5 (neuropsin) and display light responses associated to intracellular Ca2 + mobilization. These findings strongly propose MC as novel retinal photodetectors (Rios et al., 2019). Herein, we further investigated the intrinsic light responses of primary cultures of MC from embryonic chicken retinas specially focused on Ca2 + mobilization by fluorescence imaging and the identity of the internal Ca2 + stores responsible for blue light responses. Results clearly demonstrated that light responses were specific to blue light of long time exposure, and that the main Ca2 + reservoir to trigger downstream responses came from intracellular stores localized in the endoplasmic reticulum These observations bring more complexity to the intrinsic photosensitivity of retinal cells, particularly with regard to the detection of light in the blue range of visible spectra, and add novel functions to glial cells cooperating with other photoreceptors to detect and integrate ambient light in the retinal circuit and participate in cell to cell communication. Summary statement: Non-neuronal cells in the vertebrate retina, Muller glial cells, express non-canonical photopigments and sense blue light causing calcium release from intracellular stores strongly suggesting a novel intrinsic photosensitivity and new regulatory events mediating light-driven processes with yet unknown physiological implications.
format Online
Article
Text
id pubmed-8814826
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher SAGE Publications
record_format MEDLINE/PubMed
spelling pubmed-88148262022-02-05 The Intrinsic Blue Light Responses of Avian Müller Glial Cells Imply Calcium Release from Internal Stores Marchese, Natalia A. Ríos, Maximiliano N. Guido, Mario E. ASN Neuro Original Papers The retina of vertebrates is responsible for capturing light through visual (cones and rods) and non-visual photoreceptors (intrinsically photosensitive retinal ganglion cells and horizontal cells) triggering a number of essential activities associated to image- and non-image forming functions (photic entrainment of daily rhythms, pupillary light reflexes, pineal melatonin inhibition, among others). Although the retina contains diverse types of neuronal based-photoreceptors cells, originally classified as ciliary- or rhabdomeric-like types, in recent years, it has been shown that the major glial cell type of the retina, the Müller glial cells (MC), express blue photopigments as Opn3 (encephalopsin) and Opn5 (neuropsin) and display light responses associated to intracellular Ca2 + mobilization. These findings strongly propose MC as novel retinal photodetectors (Rios et al., 2019). Herein, we further investigated the intrinsic light responses of primary cultures of MC from embryonic chicken retinas specially focused on Ca2 + mobilization by fluorescence imaging and the identity of the internal Ca2 + stores responsible for blue light responses. Results clearly demonstrated that light responses were specific to blue light of long time exposure, and that the main Ca2 + reservoir to trigger downstream responses came from intracellular stores localized in the endoplasmic reticulum These observations bring more complexity to the intrinsic photosensitivity of retinal cells, particularly with regard to the detection of light in the blue range of visible spectra, and add novel functions to glial cells cooperating with other photoreceptors to detect and integrate ambient light in the retinal circuit and participate in cell to cell communication. Summary statement: Non-neuronal cells in the vertebrate retina, Muller glial cells, express non-canonical photopigments and sense blue light causing calcium release from intracellular stores strongly suggesting a novel intrinsic photosensitivity and new regulatory events mediating light-driven processes with yet unknown physiological implications. SAGE Publications 2022-02-01 /pmc/articles/PMC8814826/ /pubmed/35103506 http://dx.doi.org/10.1177/17590914221076698 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Papers
Marchese, Natalia A.
Ríos, Maximiliano N.
Guido, Mario E.
The Intrinsic Blue Light Responses of Avian Müller Glial Cells Imply Calcium Release from Internal Stores
title The Intrinsic Blue Light Responses of Avian Müller Glial Cells Imply Calcium Release from Internal Stores
title_full The Intrinsic Blue Light Responses of Avian Müller Glial Cells Imply Calcium Release from Internal Stores
title_fullStr The Intrinsic Blue Light Responses of Avian Müller Glial Cells Imply Calcium Release from Internal Stores
title_full_unstemmed The Intrinsic Blue Light Responses of Avian Müller Glial Cells Imply Calcium Release from Internal Stores
title_short The Intrinsic Blue Light Responses of Avian Müller Glial Cells Imply Calcium Release from Internal Stores
title_sort intrinsic blue light responses of avian müller glial cells imply calcium release from internal stores
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8814826/
https://www.ncbi.nlm.nih.gov/pubmed/35103506
http://dx.doi.org/10.1177/17590914221076698
work_keys_str_mv AT marchesenataliaa theintrinsicbluelightresponsesofavianmullerglialcellsimplycalciumreleasefrominternalstores
AT riosmaximilianon theintrinsicbluelightresponsesofavianmullerglialcellsimplycalciumreleasefrominternalstores
AT guidomarioe theintrinsicbluelightresponsesofavianmullerglialcellsimplycalciumreleasefrominternalstores
AT marchesenataliaa intrinsicbluelightresponsesofavianmullerglialcellsimplycalciumreleasefrominternalstores
AT riosmaximilianon intrinsicbluelightresponsesofavianmullerglialcellsimplycalciumreleasefrominternalstores
AT guidomarioe intrinsicbluelightresponsesofavianmullerglialcellsimplycalciumreleasefrominternalstores