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Peptide-Modified Nano-Bioactive Glass for Targeted Immobilization of Native VEGF

[Image: see text] A limiting factor in large bone defect regeneration is the slow and disorganized formation of a functional vascular network in the defect area, often resulting in delayed healing or implant failure. To overcome this, strategies that induce angiogenic processes should be combined wi...

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Autores principales: Schumacher, Matthias, Habibović, Pamela, van Rijt, Sabine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8815037/
https://www.ncbi.nlm.nih.gov/pubmed/35041377
http://dx.doi.org/10.1021/acsami.1c21378
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author Schumacher, Matthias
Habibović, Pamela
van Rijt, Sabine
author_facet Schumacher, Matthias
Habibović, Pamela
van Rijt, Sabine
author_sort Schumacher, Matthias
collection PubMed
description [Image: see text] A limiting factor in large bone defect regeneration is the slow and disorganized formation of a functional vascular network in the defect area, often resulting in delayed healing or implant failure. To overcome this, strategies that induce angiogenic processes should be combined with potent bone graft substitutes in new bone regeneration approaches. To this end, we describe a unique approach to immobilize the pro-angiogenic growth factor VEGF(165) in its native state on the surface of nanosized bioactive glass particles (nBGs) via a binding peptide (PR1P). We demonstrate that covalent coupling of the peptide to amine functional groups grafted on the nBG surface allows immobilization of VEGF with high efficiency and specificity. The amount of coupled peptide could be controlled by varying amine density, which eventually allows tailoring the amount of bound VEGF within a physiologically effective range. In vitro analysis of endothelial cell tube formation in response to VEGF-carrying nBG confirmed that the biological activity of VEGF is not compromised by the immobilization. Instead, comparable angiogenic stimulation was found for lower doses of immobilized VEGF compared to exogenously added VEGF. The described system, for the first time, employs a binding peptide for growth factor immobilization on bioactive glass nanoparticles and represents a promising strategy to overcome the problem of insufficient neovascularization in large bone defect regeneration.
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spelling pubmed-88150372022-02-07 Peptide-Modified Nano-Bioactive Glass for Targeted Immobilization of Native VEGF Schumacher, Matthias Habibović, Pamela van Rijt, Sabine ACS Appl Mater Interfaces [Image: see text] A limiting factor in large bone defect regeneration is the slow and disorganized formation of a functional vascular network in the defect area, often resulting in delayed healing or implant failure. To overcome this, strategies that induce angiogenic processes should be combined with potent bone graft substitutes in new bone regeneration approaches. To this end, we describe a unique approach to immobilize the pro-angiogenic growth factor VEGF(165) in its native state on the surface of nanosized bioactive glass particles (nBGs) via a binding peptide (PR1P). We demonstrate that covalent coupling of the peptide to amine functional groups grafted on the nBG surface allows immobilization of VEGF with high efficiency and specificity. The amount of coupled peptide could be controlled by varying amine density, which eventually allows tailoring the amount of bound VEGF within a physiologically effective range. In vitro analysis of endothelial cell tube formation in response to VEGF-carrying nBG confirmed that the biological activity of VEGF is not compromised by the immobilization. Instead, comparable angiogenic stimulation was found for lower doses of immobilized VEGF compared to exogenously added VEGF. The described system, for the first time, employs a binding peptide for growth factor immobilization on bioactive glass nanoparticles and represents a promising strategy to overcome the problem of insufficient neovascularization in large bone defect regeneration. American Chemical Society 2022-01-18 2022-02-02 /pmc/articles/PMC8815037/ /pubmed/35041377 http://dx.doi.org/10.1021/acsami.1c21378 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Schumacher, Matthias
Habibović, Pamela
van Rijt, Sabine
Peptide-Modified Nano-Bioactive Glass for Targeted Immobilization of Native VEGF
title Peptide-Modified Nano-Bioactive Glass for Targeted Immobilization of Native VEGF
title_full Peptide-Modified Nano-Bioactive Glass for Targeted Immobilization of Native VEGF
title_fullStr Peptide-Modified Nano-Bioactive Glass for Targeted Immobilization of Native VEGF
title_full_unstemmed Peptide-Modified Nano-Bioactive Glass for Targeted Immobilization of Native VEGF
title_short Peptide-Modified Nano-Bioactive Glass for Targeted Immobilization of Native VEGF
title_sort peptide-modified nano-bioactive glass for targeted immobilization of native vegf
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8815037/
https://www.ncbi.nlm.nih.gov/pubmed/35041377
http://dx.doi.org/10.1021/acsami.1c21378
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