Cargando…

Complete genome sequence of the kiwifruit bacterial canker pathogen Pseudomonas savastanoi strain MHT1

BACKGROUND: Pseudomonas savastanoi is an important plant pathogen that infects and causes symptoms in a variety of economically important crops, causing considerable loss of yield and quality. Because there has been no research reported to date on bacterial canker of kiwifruit (Actinidia chinensis)...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhong, Mingzhao, Sun, Yunhao, Zhang, Xianzhi, Liang, Hong, Xiong, Lina, Han, Qunxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8815115/
https://www.ncbi.nlm.nih.gov/pubmed/35120460
http://dx.doi.org/10.1186/s12866-022-02459-4
_version_ 1784645216653803520
author Zhong, Mingzhao
Sun, Yunhao
Zhang, Xianzhi
Liang, Hong
Xiong, Lina
Han, Qunxin
author_facet Zhong, Mingzhao
Sun, Yunhao
Zhang, Xianzhi
Liang, Hong
Xiong, Lina
Han, Qunxin
author_sort Zhong, Mingzhao
collection PubMed
description BACKGROUND: Pseudomonas savastanoi is an important plant pathogen that infects and causes symptoms in a variety of economically important crops, causing considerable loss of yield and quality. Because there has been no research reported to date on bacterial canker of kiwifruit (Actinidia chinensis) plants caused by P. savastanoi and, in particular, no in-depth studies of the complete genome sequence or pathogenic mechanism, long-lasting and environmentally friendly control measures against this pathogen in kiwifruit are lacking. This study therefore has both theoretical value and practical significance. RESULTS: We report the complete genome sequence of P. savastanoi strain MHT1, which was first reported as the pathogen causing bacterial canker in kiwifruit plants. The genome consists of a 6.00-Mb chromosome with 58.5% GC content and 5008 predicted genes. Comparative genome analysis of four sequenced genomes of representative P. savastanoi strains revealed that 230 genes are unique to the MHT1 strain and that these genes are enriched in antibiotic metabolic processes and metabolic pathways, which may be associated with the drug resistance and host range observed in this strain. MHT1 showed high syntenic relationships with different P. savastanoi strains. Furthermore, MHT1 has eight conserved effectors that are highly homologous to effectors from P. syringae, Pseudomonas amygdali, and Ralstonia solanacearum strains. The MHT1 genome contains six genomic islands and two prophage sequences. In addition, 380 genes were annotated as antibiotic resistance genes and another 734 as encoding carbohydrate-active enzymes. CONCLUSION: The whole-genome sequence of this kiwifruit bacterial canker pathogen extends our knowledge of the P. savastanoi genome, sets the stage for further studies of the interaction between kiwifruit and P. savastanoi, and provides an important theoretical foundation for the prevention and control of bacterial canker. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-022-02459-4.
format Online
Article
Text
id pubmed-8815115
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-88151152022-02-07 Complete genome sequence of the kiwifruit bacterial canker pathogen Pseudomonas savastanoi strain MHT1 Zhong, Mingzhao Sun, Yunhao Zhang, Xianzhi Liang, Hong Xiong, Lina Han, Qunxin BMC Microbiol Research BACKGROUND: Pseudomonas savastanoi is an important plant pathogen that infects and causes symptoms in a variety of economically important crops, causing considerable loss of yield and quality. Because there has been no research reported to date on bacterial canker of kiwifruit (Actinidia chinensis) plants caused by P. savastanoi and, in particular, no in-depth studies of the complete genome sequence or pathogenic mechanism, long-lasting and environmentally friendly control measures against this pathogen in kiwifruit are lacking. This study therefore has both theoretical value and practical significance. RESULTS: We report the complete genome sequence of P. savastanoi strain MHT1, which was first reported as the pathogen causing bacterial canker in kiwifruit plants. The genome consists of a 6.00-Mb chromosome with 58.5% GC content and 5008 predicted genes. Comparative genome analysis of four sequenced genomes of representative P. savastanoi strains revealed that 230 genes are unique to the MHT1 strain and that these genes are enriched in antibiotic metabolic processes and metabolic pathways, which may be associated with the drug resistance and host range observed in this strain. MHT1 showed high syntenic relationships with different P. savastanoi strains. Furthermore, MHT1 has eight conserved effectors that are highly homologous to effectors from P. syringae, Pseudomonas amygdali, and Ralstonia solanacearum strains. The MHT1 genome contains six genomic islands and two prophage sequences. In addition, 380 genes were annotated as antibiotic resistance genes and another 734 as encoding carbohydrate-active enzymes. CONCLUSION: The whole-genome sequence of this kiwifruit bacterial canker pathogen extends our knowledge of the P. savastanoi genome, sets the stage for further studies of the interaction between kiwifruit and P. savastanoi, and provides an important theoretical foundation for the prevention and control of bacterial canker. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-022-02459-4. BioMed Central 2022-02-04 /pmc/articles/PMC8815115/ /pubmed/35120460 http://dx.doi.org/10.1186/s12866-022-02459-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhong, Mingzhao
Sun, Yunhao
Zhang, Xianzhi
Liang, Hong
Xiong, Lina
Han, Qunxin
Complete genome sequence of the kiwifruit bacterial canker pathogen Pseudomonas savastanoi strain MHT1
title Complete genome sequence of the kiwifruit bacterial canker pathogen Pseudomonas savastanoi strain MHT1
title_full Complete genome sequence of the kiwifruit bacterial canker pathogen Pseudomonas savastanoi strain MHT1
title_fullStr Complete genome sequence of the kiwifruit bacterial canker pathogen Pseudomonas savastanoi strain MHT1
title_full_unstemmed Complete genome sequence of the kiwifruit bacterial canker pathogen Pseudomonas savastanoi strain MHT1
title_short Complete genome sequence of the kiwifruit bacterial canker pathogen Pseudomonas savastanoi strain MHT1
title_sort complete genome sequence of the kiwifruit bacterial canker pathogen pseudomonas savastanoi strain mht1
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8815115/
https://www.ncbi.nlm.nih.gov/pubmed/35120460
http://dx.doi.org/10.1186/s12866-022-02459-4
work_keys_str_mv AT zhongmingzhao completegenomesequenceofthekiwifruitbacterialcankerpathogenpseudomonassavastanoistrainmht1
AT sunyunhao completegenomesequenceofthekiwifruitbacterialcankerpathogenpseudomonassavastanoistrainmht1
AT zhangxianzhi completegenomesequenceofthekiwifruitbacterialcankerpathogenpseudomonassavastanoistrainmht1
AT lianghong completegenomesequenceofthekiwifruitbacterialcankerpathogenpseudomonassavastanoistrainmht1
AT xionglina completegenomesequenceofthekiwifruitbacterialcankerpathogenpseudomonassavastanoistrainmht1
AT hanqunxin completegenomesequenceofthekiwifruitbacterialcankerpathogenpseudomonassavastanoistrainmht1