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Enrichment of phosphate-accumulating organisms (PAOs) in a microfluidic model biofilm system by mimicking a typical aerobic granular sludge feast/famine regime

Wastewater treatment using aerobic granular sludge has gained increasing interest due to its advantages compared to conventional activated sludge. The technology allows simultaneous removal of organic carbon, nitrogen, and phosphorus in a single reactor system and is independent of space-intensive s...

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Autores principales: Klein, Edina, Weiler, Janek, Wagner, Michael, Čelikić, Minja, Niemeyer, Christof M., Horn, Harald, Gescher, Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8816403/
https://www.ncbi.nlm.nih.gov/pubmed/35032186
http://dx.doi.org/10.1007/s00253-022-11759-8
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author Klein, Edina
Weiler, Janek
Wagner, Michael
Čelikić, Minja
Niemeyer, Christof M.
Horn, Harald
Gescher, Johannes
author_facet Klein, Edina
Weiler, Janek
Wagner, Michael
Čelikić, Minja
Niemeyer, Christof M.
Horn, Harald
Gescher, Johannes
author_sort Klein, Edina
collection PubMed
description Wastewater treatment using aerobic granular sludge has gained increasing interest due to its advantages compared to conventional activated sludge. The technology allows simultaneous removal of organic carbon, nitrogen, and phosphorus in a single reactor system and is independent of space-intensive settling tanks. However, due to the microscale, an analysis of processes and microbial population along the radius of granules is challenging. Here, we introduce a model system for aerobic granular sludge on a small scale by using a machine-assisted microfluidic cultivation platform. With an implemented logic module that controls solenoid valves, we realized alternating oxic hunger and anoxic feeding phases for the biofilms growing within. Sampling during ongoing anoxic cultivation directly from the cultivation channel was achieved with a robotic sampling device. Analysis of the biofilms was conducted using optical coherence tomography, fluorescence in situ hybridization, and amplicon sequencing. Using this setup, it was possible to significantly enrich the percentage of polyphosphate-accumulating organisms (PAO) belonging to the family Rhodocyclaceae in the community compared to the starting inoculum. With the aid of this miniature model system, it is now possible to investigate the influence of a multitude of process parameters in a highly parallel way to understand and efficiently optimize aerobic granular sludge-based wastewater treatment systems. Key points • Development of a microfluidic model to study EBPR. • Feast-famine regime enriches polyphosphate-accumulating organisms (PAOs). • Microfluidics replace sequencing batch reactors for aerobic granular sludge research. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-022-11759-8.
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spelling pubmed-88164032022-02-11 Enrichment of phosphate-accumulating organisms (PAOs) in a microfluidic model biofilm system by mimicking a typical aerobic granular sludge feast/famine regime Klein, Edina Weiler, Janek Wagner, Michael Čelikić, Minja Niemeyer, Christof M. Horn, Harald Gescher, Johannes Appl Microbiol Biotechnol Environmental Biotechnology Wastewater treatment using aerobic granular sludge has gained increasing interest due to its advantages compared to conventional activated sludge. The technology allows simultaneous removal of organic carbon, nitrogen, and phosphorus in a single reactor system and is independent of space-intensive settling tanks. However, due to the microscale, an analysis of processes and microbial population along the radius of granules is challenging. Here, we introduce a model system for aerobic granular sludge on a small scale by using a machine-assisted microfluidic cultivation platform. With an implemented logic module that controls solenoid valves, we realized alternating oxic hunger and anoxic feeding phases for the biofilms growing within. Sampling during ongoing anoxic cultivation directly from the cultivation channel was achieved with a robotic sampling device. Analysis of the biofilms was conducted using optical coherence tomography, fluorescence in situ hybridization, and amplicon sequencing. Using this setup, it was possible to significantly enrich the percentage of polyphosphate-accumulating organisms (PAO) belonging to the family Rhodocyclaceae in the community compared to the starting inoculum. With the aid of this miniature model system, it is now possible to investigate the influence of a multitude of process parameters in a highly parallel way to understand and efficiently optimize aerobic granular sludge-based wastewater treatment systems. Key points • Development of a microfluidic model to study EBPR. • Feast-famine regime enriches polyphosphate-accumulating organisms (PAOs). • Microfluidics replace sequencing batch reactors for aerobic granular sludge research. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-022-11759-8. Springer Berlin Heidelberg 2022-01-15 2022 /pmc/articles/PMC8816403/ /pubmed/35032186 http://dx.doi.org/10.1007/s00253-022-11759-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Environmental Biotechnology
Klein, Edina
Weiler, Janek
Wagner, Michael
Čelikić, Minja
Niemeyer, Christof M.
Horn, Harald
Gescher, Johannes
Enrichment of phosphate-accumulating organisms (PAOs) in a microfluidic model biofilm system by mimicking a typical aerobic granular sludge feast/famine regime
title Enrichment of phosphate-accumulating organisms (PAOs) in a microfluidic model biofilm system by mimicking a typical aerobic granular sludge feast/famine regime
title_full Enrichment of phosphate-accumulating organisms (PAOs) in a microfluidic model biofilm system by mimicking a typical aerobic granular sludge feast/famine regime
title_fullStr Enrichment of phosphate-accumulating organisms (PAOs) in a microfluidic model biofilm system by mimicking a typical aerobic granular sludge feast/famine regime
title_full_unstemmed Enrichment of phosphate-accumulating organisms (PAOs) in a microfluidic model biofilm system by mimicking a typical aerobic granular sludge feast/famine regime
title_short Enrichment of phosphate-accumulating organisms (PAOs) in a microfluidic model biofilm system by mimicking a typical aerobic granular sludge feast/famine regime
title_sort enrichment of phosphate-accumulating organisms (paos) in a microfluidic model biofilm system by mimicking a typical aerobic granular sludge feast/famine regime
topic Environmental Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8816403/
https://www.ncbi.nlm.nih.gov/pubmed/35032186
http://dx.doi.org/10.1007/s00253-022-11759-8
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