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Expression and significance of SOX B1 genes in glioblastoma multiforme patients
The overall survival of glioblastoma multiforme (GBM) patients remains poor. To improve patient outcomes, effective diagnostic and prognostic biomarkers for GBM are needed. In this study, we first applied bioinformatic analyses to identify biomarkers for GBM, focusing on SOX (sex‐determining region...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8817144/ https://www.ncbi.nlm.nih.gov/pubmed/34953010 http://dx.doi.org/10.1111/jcmm.17120 |
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author | Pan, Cunyao Liang, Lanlan Wang, Zirou Zhang, Baoyi Li, Qionglin Tian, Yingrui Yu, Yijing Chen, Zhaoli Wang, Xinxing Liu, Hui |
author_facet | Pan, Cunyao Liang, Lanlan Wang, Zirou Zhang, Baoyi Li, Qionglin Tian, Yingrui Yu, Yijing Chen, Zhaoli Wang, Xinxing Liu, Hui |
author_sort | Pan, Cunyao |
collection | PubMed |
description | The overall survival of glioblastoma multiforme (GBM) patients remains poor. To improve patient outcomes, effective diagnostic and prognostic biomarkers for GBM are needed. In this study, we first applied bioinformatic analyses to identify biomarkers for GBM, focusing on SOX (sex‐determining region on the Y chromosome (SRY)‐related high mobility group (HMG) box) B1 family members. The ONCOMINE, GEPIA, LinkedOmics and CCLE databases were used to assess mRNA expression levels of the SOX B1 family members in different cancers and normal tissue. Further bioinformatic analysis was performed using the ONCOMINE database in combination with the LinkedOmics data set to identify the prognostic value of SOX B1 family members for GBM. We found mRNA expression levels of all tested SOX B1 genes were significantly increased in GBM. In the LinkedOmics database, increased expression of SOX3 indicated a better overall survival. In GEPIA databases, increased expression of all SOX B1 family members suggested an improved overall survival, but none of them were statistically different. Then, Transwell assays and wound healing were employed to evaluate the motility and invasive captivity of U251 cells when silencing SOX2 and SOX3. We found exogenous inhibition of SOX2 appeared to reduce the migration and invasion of U251 cells in vitro. Collectively, our research suggested that SOX2 might serve as a cancer‐promoting gene to identify high‐risk GBM patients, and SOX3 had the potential to be a prognostic biomarker for GBM patients. |
format | Online Article Text |
id | pubmed-8817144 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88171442022-02-08 Expression and significance of SOX B1 genes in glioblastoma multiforme patients Pan, Cunyao Liang, Lanlan Wang, Zirou Zhang, Baoyi Li, Qionglin Tian, Yingrui Yu, Yijing Chen, Zhaoli Wang, Xinxing Liu, Hui J Cell Mol Med Original Articles The overall survival of glioblastoma multiforme (GBM) patients remains poor. To improve patient outcomes, effective diagnostic and prognostic biomarkers for GBM are needed. In this study, we first applied bioinformatic analyses to identify biomarkers for GBM, focusing on SOX (sex‐determining region on the Y chromosome (SRY)‐related high mobility group (HMG) box) B1 family members. The ONCOMINE, GEPIA, LinkedOmics and CCLE databases were used to assess mRNA expression levels of the SOX B1 family members in different cancers and normal tissue. Further bioinformatic analysis was performed using the ONCOMINE database in combination with the LinkedOmics data set to identify the prognostic value of SOX B1 family members for GBM. We found mRNA expression levels of all tested SOX B1 genes were significantly increased in GBM. In the LinkedOmics database, increased expression of SOX3 indicated a better overall survival. In GEPIA databases, increased expression of all SOX B1 family members suggested an improved overall survival, but none of them were statistically different. Then, Transwell assays and wound healing were employed to evaluate the motility and invasive captivity of U251 cells when silencing SOX2 and SOX3. We found exogenous inhibition of SOX2 appeared to reduce the migration and invasion of U251 cells in vitro. Collectively, our research suggested that SOX2 might serve as a cancer‐promoting gene to identify high‐risk GBM patients, and SOX3 had the potential to be a prognostic biomarker for GBM patients. John Wiley and Sons Inc. 2021-12-24 2022-02 /pmc/articles/PMC8817144/ /pubmed/34953010 http://dx.doi.org/10.1111/jcmm.17120 Text en © 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Pan, Cunyao Liang, Lanlan Wang, Zirou Zhang, Baoyi Li, Qionglin Tian, Yingrui Yu, Yijing Chen, Zhaoli Wang, Xinxing Liu, Hui Expression and significance of SOX B1 genes in glioblastoma multiforme patients |
title | Expression and significance of SOX B1 genes in glioblastoma multiforme patients |
title_full | Expression and significance of SOX B1 genes in glioblastoma multiforme patients |
title_fullStr | Expression and significance of SOX B1 genes in glioblastoma multiforme patients |
title_full_unstemmed | Expression and significance of SOX B1 genes in glioblastoma multiforme patients |
title_short | Expression and significance of SOX B1 genes in glioblastoma multiforme patients |
title_sort | expression and significance of sox b1 genes in glioblastoma multiforme patients |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8817144/ https://www.ncbi.nlm.nih.gov/pubmed/34953010 http://dx.doi.org/10.1111/jcmm.17120 |
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