Metabolic engineering of Escherichia coli BL21 strain using simplified CRISPR-Cas9 and asymmetric homology arms recombineering

BACKGROUND: The recent CRISPR-Cas coupled with λ recombinase mediated genome recombineering has become a common laboratory practice to modify bacterial genomes. It requires supplying a template DNA with homology arms for precise genome editing. However, generation of homology arms is a time-consumin...

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Detalles Bibliográficos
Autores principales: Shukal, Sudha, Lim, Xiao Hui, Zhang, Congqiang, Chen, Xixian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8817497/
https://www.ncbi.nlm.nih.gov/pubmed/35123478
http://dx.doi.org/10.1186/s12934-022-01746-z

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