Development of a liquid phase radioimmunoassay for the measurement of serum ferritin levels for the detection of Covid-19 in patients

The aim of this study was the development and analytically validation of a radioimmunoassay system for the measurement of the serum ferritin concentration as one of the laboratory biomarkers for infection by Covid-19. The main components of the system were prepared in our laboratories. The first com...

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Autores principales: Riad, Emil Michael, Emam, Manal Asem, Ebeid, Nahed Hassan, El-bayoumy, Ahmed Sami, Sallam, Khaled Mohamed, Mehany, Nagy Lahzy, Fathy, Shadia Abdel-Hamid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8817774/
https://www.ncbi.nlm.nih.gov/pubmed/35153356
http://dx.doi.org/10.1007/s10967-022-08208-1
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author Riad, Emil Michael
Emam, Manal Asem
Ebeid, Nahed Hassan
El-bayoumy, Ahmed Sami
Sallam, Khaled Mohamed
Mehany, Nagy Lahzy
Fathy, Shadia Abdel-Hamid
author_facet Riad, Emil Michael
Emam, Manal Asem
Ebeid, Nahed Hassan
El-bayoumy, Ahmed Sami
Sallam, Khaled Mohamed
Mehany, Nagy Lahzy
Fathy, Shadia Abdel-Hamid
author_sort Riad, Emil Michael
collection PubMed
description The aim of this study was the development and analytically validation of a radioimmunoassay system for the measurement of the serum ferritin concentration as one of the laboratory biomarkers for infection by Covid-19. The main components of the system were prepared in our laboratories. The first component ferritin was extracted and purified from human spleen with high purity. The second component was the 125I-labelled ferritin tracer, prepared using Chloramine-T method. Furthermore anti-ferritin antibodies and ferritin standards were provided. The developed system is sensitive, precise, reproducible and. can be translated into a kit formulation suitable for measuring serum ferritin for the detection of Covid-19 in patients at low costs and high efficiency.
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spelling pubmed-88177742022-02-07 Development of a liquid phase radioimmunoassay for the measurement of serum ferritin levels for the detection of Covid-19 in patients Riad, Emil Michael Emam, Manal Asem Ebeid, Nahed Hassan El-bayoumy, Ahmed Sami Sallam, Khaled Mohamed Mehany, Nagy Lahzy Fathy, Shadia Abdel-Hamid J Radioanal Nucl Chem Article The aim of this study was the development and analytically validation of a radioimmunoassay system for the measurement of the serum ferritin concentration as one of the laboratory biomarkers for infection by Covid-19. The main components of the system were prepared in our laboratories. The first component ferritin was extracted and purified from human spleen with high purity. The second component was the 125I-labelled ferritin tracer, prepared using Chloramine-T method. Furthermore anti-ferritin antibodies and ferritin standards were provided. The developed system is sensitive, precise, reproducible and. can be translated into a kit formulation suitable for measuring serum ferritin for the detection of Covid-19 in patients at low costs and high efficiency. Springer International Publishing 2022-02-05 2022 /pmc/articles/PMC8817774/ /pubmed/35153356 http://dx.doi.org/10.1007/s10967-022-08208-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Riad, Emil Michael
Emam, Manal Asem
Ebeid, Nahed Hassan
El-bayoumy, Ahmed Sami
Sallam, Khaled Mohamed
Mehany, Nagy Lahzy
Fathy, Shadia Abdel-Hamid
Development of a liquid phase radioimmunoassay for the measurement of serum ferritin levels for the detection of Covid-19 in patients
title Development of a liquid phase radioimmunoassay for the measurement of serum ferritin levels for the detection of Covid-19 in patients
title_full Development of a liquid phase radioimmunoassay for the measurement of serum ferritin levels for the detection of Covid-19 in patients
title_fullStr Development of a liquid phase radioimmunoassay for the measurement of serum ferritin levels for the detection of Covid-19 in patients
title_full_unstemmed Development of a liquid phase radioimmunoassay for the measurement of serum ferritin levels for the detection of Covid-19 in patients
title_short Development of a liquid phase radioimmunoassay for the measurement of serum ferritin levels for the detection of Covid-19 in patients
title_sort development of a liquid phase radioimmunoassay for the measurement of serum ferritin levels for the detection of covid-19 in patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8817774/
https://www.ncbi.nlm.nih.gov/pubmed/35153356
http://dx.doi.org/10.1007/s10967-022-08208-1
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