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Evaluation of the GABAA Receptor Expression and the Effects of Muscimol on the Activity of Wide Dynamic Range Neurons Following Chronic Constriction Injury of Sciatic Nerve in Rats

INTRODUCTION: The modality of γ-aminobutyric acid type a receptors (GABAA) controls dorsal horn neuronal excitability and inhibits sensory information. This study aimed to investigate the expression of the GABAA receptor and the effects of its agonist muscimol on Wide Dynamic Range (WDR) neuronal ac...

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Autores principales: Sadeghi, Mehdi, Manaheji, Homa, Zaringhalam, Jalal, Haghparast, Abbas, Nazemi, Samad, Bahari, Zahra, Noorbakhsh, Seyed Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Neuroscience Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8818116/
https://www.ncbi.nlm.nih.gov/pubmed/35173919
http://dx.doi.org/10.32598/bcn.2021.1726.1
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author Sadeghi, Mehdi
Manaheji, Homa
Zaringhalam, Jalal
Haghparast, Abbas
Nazemi, Samad
Bahari, Zahra
Noorbakhsh, Seyed Mohammad
author_facet Sadeghi, Mehdi
Manaheji, Homa
Zaringhalam, Jalal
Haghparast, Abbas
Nazemi, Samad
Bahari, Zahra
Noorbakhsh, Seyed Mohammad
author_sort Sadeghi, Mehdi
collection PubMed
description INTRODUCTION: The modality of γ-aminobutyric acid type a receptors (GABAA) controls dorsal horn neuronal excitability and inhibits sensory information. This study aimed to investigate the expression of the GABAA receptor and the effects of its agonist muscimol on Wide Dynamic Range (WDR) neuronal activity in the Chronic Constriction Injury (CCI) model of neuropathic pain. METHODS: Adult male Wistar rats weighing 200 to 250 g were used to induce CCI neuropathy. Fourteen days after surgery, muscimol (0.5, 1, and 2 mg/kg IP) was injected. Then, the behavioral tests were performed. After that, the animals were killed, and the lumbar segments of the spinal cords were collected for Western blot analysis of the GABAA receptor α1 subunit expression. The electrophysiological properties of WDR neurons were studied by single-unit recordings in separate groups 14 days after CCI. RESULTS: The outcomes indicated the development of thermal hyperalgesia and mechanical allodynia after neuropathy; nonetheless, the expression of the GABAA receptor α1 subunit did not change significantly. Moreover, the evoked responses of the WDR neurons to electrical, mechanical, and thermal stimuli increased considerably. Fourteen days after CCI, muscimol administration decreased thermal hyperalgesia, mechanical allodynia, and hyper-responsiveness of the WDR neurons in CCI rats. CONCLUSION: The modulation of the spinal GABAA receptors after nerve injury can offer further insights to design new therapeutic agents to reduce neuropathic pain symptoms.
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spelling pubmed-88181162022-02-15 Evaluation of the GABAA Receptor Expression and the Effects of Muscimol on the Activity of Wide Dynamic Range Neurons Following Chronic Constriction Injury of Sciatic Nerve in Rats Sadeghi, Mehdi Manaheji, Homa Zaringhalam, Jalal Haghparast, Abbas Nazemi, Samad Bahari, Zahra Noorbakhsh, Seyed Mohammad Basic Clin Neurosci Research Paper INTRODUCTION: The modality of γ-aminobutyric acid type a receptors (GABAA) controls dorsal horn neuronal excitability and inhibits sensory information. This study aimed to investigate the expression of the GABAA receptor and the effects of its agonist muscimol on Wide Dynamic Range (WDR) neuronal activity in the Chronic Constriction Injury (CCI) model of neuropathic pain. METHODS: Adult male Wistar rats weighing 200 to 250 g were used to induce CCI neuropathy. Fourteen days after surgery, muscimol (0.5, 1, and 2 mg/kg IP) was injected. Then, the behavioral tests were performed. After that, the animals were killed, and the lumbar segments of the spinal cords were collected for Western blot analysis of the GABAA receptor α1 subunit expression. The electrophysiological properties of WDR neurons were studied by single-unit recordings in separate groups 14 days after CCI. RESULTS: The outcomes indicated the development of thermal hyperalgesia and mechanical allodynia after neuropathy; nonetheless, the expression of the GABAA receptor α1 subunit did not change significantly. Moreover, the evoked responses of the WDR neurons to electrical, mechanical, and thermal stimuli increased considerably. Fourteen days after CCI, muscimol administration decreased thermal hyperalgesia, mechanical allodynia, and hyper-responsiveness of the WDR neurons in CCI rats. CONCLUSION: The modulation of the spinal GABAA receptors after nerve injury can offer further insights to design new therapeutic agents to reduce neuropathic pain symptoms. Iranian Neuroscience Society 2021 2021-09-01 /pmc/articles/PMC8818116/ /pubmed/35173919 http://dx.doi.org/10.32598/bcn.2021.1726.1 Text en Copyright© 2021 Iranian Neuroscience Society https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/)
spellingShingle Research Paper
Sadeghi, Mehdi
Manaheji, Homa
Zaringhalam, Jalal
Haghparast, Abbas
Nazemi, Samad
Bahari, Zahra
Noorbakhsh, Seyed Mohammad
Evaluation of the GABAA Receptor Expression and the Effects of Muscimol on the Activity of Wide Dynamic Range Neurons Following Chronic Constriction Injury of Sciatic Nerve in Rats
title Evaluation of the GABAA Receptor Expression and the Effects of Muscimol on the Activity of Wide Dynamic Range Neurons Following Chronic Constriction Injury of Sciatic Nerve in Rats
title_full Evaluation of the GABAA Receptor Expression and the Effects of Muscimol on the Activity of Wide Dynamic Range Neurons Following Chronic Constriction Injury of Sciatic Nerve in Rats
title_fullStr Evaluation of the GABAA Receptor Expression and the Effects of Muscimol on the Activity of Wide Dynamic Range Neurons Following Chronic Constriction Injury of Sciatic Nerve in Rats
title_full_unstemmed Evaluation of the GABAA Receptor Expression and the Effects of Muscimol on the Activity of Wide Dynamic Range Neurons Following Chronic Constriction Injury of Sciatic Nerve in Rats
title_short Evaluation of the GABAA Receptor Expression and the Effects of Muscimol on the Activity of Wide Dynamic Range Neurons Following Chronic Constriction Injury of Sciatic Nerve in Rats
title_sort evaluation of the gabaa receptor expression and the effects of muscimol on the activity of wide dynamic range neurons following chronic constriction injury of sciatic nerve in rats
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8818116/
https://www.ncbi.nlm.nih.gov/pubmed/35173919
http://dx.doi.org/10.32598/bcn.2021.1726.1
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