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Treatment of bladder cancer by geoinspired synthetic chrysotile nanocarrier-delivered circPRMT5 siRNA

BACKGROUND: Circular RNAs (circRNAs) have important functions in many fields of cancer biology. In particular, we previously reported that the oncogenic circRNA, circPRMT5, has a major role in bladder cancer progression. Therapy based on circRNAs have good prospects as anticancer strategies. While a...

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Autores principales: Yu, Chunping, Zhang, Yi, Wang, Ning, Wei, Wensu, Cao, Ke, Zhang, Qun, Ma, Peiying, Xie, Dan, Wu, Pei, Liu, Biao, Liu, Jiahao, Xiang, Wei, Hu, Xing, Liu, Xuewen, Xie, Jianfei, Tang, Jin, Long, Zhi, Wang, Long, Zeng, Hongliang, Liu, Jianye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8818206/
https://www.ncbi.nlm.nih.gov/pubmed/35123588
http://dx.doi.org/10.1186/s40824-022-00251-z
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author Yu, Chunping
Zhang, Yi
Wang, Ning
Wei, Wensu
Cao, Ke
Zhang, Qun
Ma, Peiying
Xie, Dan
Wu, Pei
Liu, Biao
Liu, Jiahao
Xiang, Wei
Hu, Xing
Liu, Xuewen
Xie, Jianfei
Tang, Jin
Long, Zhi
Wang, Long
Zeng, Hongliang
Liu, Jianye
author_facet Yu, Chunping
Zhang, Yi
Wang, Ning
Wei, Wensu
Cao, Ke
Zhang, Qun
Ma, Peiying
Xie, Dan
Wu, Pei
Liu, Biao
Liu, Jiahao
Xiang, Wei
Hu, Xing
Liu, Xuewen
Xie, Jianfei
Tang, Jin
Long, Zhi
Wang, Long
Zeng, Hongliang
Liu, Jianye
author_sort Yu, Chunping
collection PubMed
description BACKGROUND: Circular RNAs (circRNAs) have important functions in many fields of cancer biology. In particular, we previously reported that the oncogenic circRNA, circPRMT5, has a major role in bladder cancer progression. Therapy based on circRNAs have good prospects as anticancer strategies. While anti-circRNAs are emerging as therapeutics, the specific in vivo delivery of anti-circRNAs into cancer cells has not been reported and remains challenging. METHODS: Synthesized chrysotile nanotubes (SCNTs) with a relatively uniform length (~ 200 nm) have been designed to deliver an siRNA against the oncogenic circPRMT5 (si-circPRMT5) inhibit circPRMT5. In addition, the antitumor effects and safety evaluation of SCNTs/si-circPRMT5 was assessed with a series of in vitro and in vivo assays. RESULTS: The results showed that SCNTs/si-circPRMT5 nanomaterials prolong si-circPRMT5’s half-life in circulation, enhance its specific uptake by tumor cells, and maximize the silencing efficiency of circPRMT5. In vitro, SCNTs encapsulating si-circPRMT5 could inhibit bladder cancer cell growth and progression. In vivo, SCNTs/si-circPRMT5 inhibited growth and metastasis in three bladder tumor models (a subcutaneous model, a tail vein injection lung metastatic model, and an in situ model) without obvious toxicities. Mechanistic study showed that SCNTs/si-circPRMT5 regulated the miR-30c/SNAIL1/E-cadherin axis, inhibiting bladder cancer growth and progression. CONCLUSION: The results highlight the potential therapeutic utility of SCNTs/si-circPRMT5 to deliver si-circPRMT5 to treat bladder cancer. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40824-022-00251-z.
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spelling pubmed-88182062022-02-07 Treatment of bladder cancer by geoinspired synthetic chrysotile nanocarrier-delivered circPRMT5 siRNA Yu, Chunping Zhang, Yi Wang, Ning Wei, Wensu Cao, Ke Zhang, Qun Ma, Peiying Xie, Dan Wu, Pei Liu, Biao Liu, Jiahao Xiang, Wei Hu, Xing Liu, Xuewen Xie, Jianfei Tang, Jin Long, Zhi Wang, Long Zeng, Hongliang Liu, Jianye Biomater Res Research Article BACKGROUND: Circular RNAs (circRNAs) have important functions in many fields of cancer biology. In particular, we previously reported that the oncogenic circRNA, circPRMT5, has a major role in bladder cancer progression. Therapy based on circRNAs have good prospects as anticancer strategies. While anti-circRNAs are emerging as therapeutics, the specific in vivo delivery of anti-circRNAs into cancer cells has not been reported and remains challenging. METHODS: Synthesized chrysotile nanotubes (SCNTs) with a relatively uniform length (~ 200 nm) have been designed to deliver an siRNA against the oncogenic circPRMT5 (si-circPRMT5) inhibit circPRMT5. In addition, the antitumor effects and safety evaluation of SCNTs/si-circPRMT5 was assessed with a series of in vitro and in vivo assays. RESULTS: The results showed that SCNTs/si-circPRMT5 nanomaterials prolong si-circPRMT5’s half-life in circulation, enhance its specific uptake by tumor cells, and maximize the silencing efficiency of circPRMT5. In vitro, SCNTs encapsulating si-circPRMT5 could inhibit bladder cancer cell growth and progression. In vivo, SCNTs/si-circPRMT5 inhibited growth and metastasis in three bladder tumor models (a subcutaneous model, a tail vein injection lung metastatic model, and an in situ model) without obvious toxicities. Mechanistic study showed that SCNTs/si-circPRMT5 regulated the miR-30c/SNAIL1/E-cadherin axis, inhibiting bladder cancer growth and progression. CONCLUSION: The results highlight the potential therapeutic utility of SCNTs/si-circPRMT5 to deliver si-circPRMT5 to treat bladder cancer. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40824-022-00251-z. BioMed Central 2022-02-05 /pmc/articles/PMC8818206/ /pubmed/35123588 http://dx.doi.org/10.1186/s40824-022-00251-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Yu, Chunping
Zhang, Yi
Wang, Ning
Wei, Wensu
Cao, Ke
Zhang, Qun
Ma, Peiying
Xie, Dan
Wu, Pei
Liu, Biao
Liu, Jiahao
Xiang, Wei
Hu, Xing
Liu, Xuewen
Xie, Jianfei
Tang, Jin
Long, Zhi
Wang, Long
Zeng, Hongliang
Liu, Jianye
Treatment of bladder cancer by geoinspired synthetic chrysotile nanocarrier-delivered circPRMT5 siRNA
title Treatment of bladder cancer by geoinspired synthetic chrysotile nanocarrier-delivered circPRMT5 siRNA
title_full Treatment of bladder cancer by geoinspired synthetic chrysotile nanocarrier-delivered circPRMT5 siRNA
title_fullStr Treatment of bladder cancer by geoinspired synthetic chrysotile nanocarrier-delivered circPRMT5 siRNA
title_full_unstemmed Treatment of bladder cancer by geoinspired synthetic chrysotile nanocarrier-delivered circPRMT5 siRNA
title_short Treatment of bladder cancer by geoinspired synthetic chrysotile nanocarrier-delivered circPRMT5 siRNA
title_sort treatment of bladder cancer by geoinspired synthetic chrysotile nanocarrier-delivered circprmt5 sirna
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8818206/
https://www.ncbi.nlm.nih.gov/pubmed/35123588
http://dx.doi.org/10.1186/s40824-022-00251-z
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