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LGR5 expression and clinicopathological features of the invasive front in the fat infiltration area of pancreatic cancer

BACKGROUND: Leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5) is a strong cancer stem cell marker in colorectal cancer; however, there are many unclear aspects of LGR5 expression in pancreatic cancer. It has been reported that the interaction between tumor cells and stroma at the fa...

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Autores principales: Kamakura, Masato, Uehara, Takeshi, Iwaya, Mai, Asaka, Shiho, Kobayashi, Shota, Nakajima, Tomoyuki, Kinugawa, Yasuhiro, Nagaya, Tadanobu, Yoshizawa, Takahiro, Shimizu, Akira, Ota, Hiroyoshi, Umemura, Takeji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8818226/
https://www.ncbi.nlm.nih.gov/pubmed/35123536
http://dx.doi.org/10.1186/s13000-022-01203-w
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author Kamakura, Masato
Uehara, Takeshi
Iwaya, Mai
Asaka, Shiho
Kobayashi, Shota
Nakajima, Tomoyuki
Kinugawa, Yasuhiro
Nagaya, Tadanobu
Yoshizawa, Takahiro
Shimizu, Akira
Ota, Hiroyoshi
Umemura, Takeji
author_facet Kamakura, Masato
Uehara, Takeshi
Iwaya, Mai
Asaka, Shiho
Kobayashi, Shota
Nakajima, Tomoyuki
Kinugawa, Yasuhiro
Nagaya, Tadanobu
Yoshizawa, Takahiro
Shimizu, Akira
Ota, Hiroyoshi
Umemura, Takeji
author_sort Kamakura, Masato
collection PubMed
description BACKGROUND: Leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5) is a strong cancer stem cell marker in colorectal cancer; however, there are many unclear aspects of LGR5 expression in pancreatic cancer. It has been reported that the interaction between tumor cells and stroma at the fat infiltration site has a significant effect on pancreatic cancer prognosis. Therefore, we report a clinicopathological study of LGR5 expression at the fat invasion front in pancreatic cancer. METHODS: LGR5 expression was analyzed in 40 pancreatic ductal adenocarcinoma cases with RNAscope, which is a newly developed high-sensitivity in situ hybridization method. Epithelial-mesenchymal transition (EMT) was analyzed by the expression of E-cadherin and vimentin via immunohistochemistry. RESULTS: LGR5-positive dots were identified in all cases, especially with glandular formation. In the fat invasion front, a high histological grade showed significantly reduced LGR5 expression compared with a low histological grade (p=0.0126). LGR5 expression was significantly higher in the non-EMT phenotype group than in EMT phenotype group (p=0.0003). Additionally, LGR5 expression was significantly lower in cases with high vascular invasion than in those with low vascular invasion (p=0.0244). CONCLUSIONS: These findings suggest that decreased LGR5 expression in the fat invasion front is associated with more aggressive biological behavior in pancreatic ductal adenocarcinoma, with higher tumor grade, EMT phenotype, and higher vascular invasion.
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spelling pubmed-88182262022-02-07 LGR5 expression and clinicopathological features of the invasive front in the fat infiltration area of pancreatic cancer Kamakura, Masato Uehara, Takeshi Iwaya, Mai Asaka, Shiho Kobayashi, Shota Nakajima, Tomoyuki Kinugawa, Yasuhiro Nagaya, Tadanobu Yoshizawa, Takahiro Shimizu, Akira Ota, Hiroyoshi Umemura, Takeji Diagn Pathol Research BACKGROUND: Leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5) is a strong cancer stem cell marker in colorectal cancer; however, there are many unclear aspects of LGR5 expression in pancreatic cancer. It has been reported that the interaction between tumor cells and stroma at the fat infiltration site has a significant effect on pancreatic cancer prognosis. Therefore, we report a clinicopathological study of LGR5 expression at the fat invasion front in pancreatic cancer. METHODS: LGR5 expression was analyzed in 40 pancreatic ductal adenocarcinoma cases with RNAscope, which is a newly developed high-sensitivity in situ hybridization method. Epithelial-mesenchymal transition (EMT) was analyzed by the expression of E-cadherin and vimentin via immunohistochemistry. RESULTS: LGR5-positive dots were identified in all cases, especially with glandular formation. In the fat invasion front, a high histological grade showed significantly reduced LGR5 expression compared with a low histological grade (p=0.0126). LGR5 expression was significantly higher in the non-EMT phenotype group than in EMT phenotype group (p=0.0003). Additionally, LGR5 expression was significantly lower in cases with high vascular invasion than in those with low vascular invasion (p=0.0244). CONCLUSIONS: These findings suggest that decreased LGR5 expression in the fat invasion front is associated with more aggressive biological behavior in pancreatic ductal adenocarcinoma, with higher tumor grade, EMT phenotype, and higher vascular invasion. BioMed Central 2022-02-05 /pmc/articles/PMC8818226/ /pubmed/35123536 http://dx.doi.org/10.1186/s13000-022-01203-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Kamakura, Masato
Uehara, Takeshi
Iwaya, Mai
Asaka, Shiho
Kobayashi, Shota
Nakajima, Tomoyuki
Kinugawa, Yasuhiro
Nagaya, Tadanobu
Yoshizawa, Takahiro
Shimizu, Akira
Ota, Hiroyoshi
Umemura, Takeji
LGR5 expression and clinicopathological features of the invasive front in the fat infiltration area of pancreatic cancer
title LGR5 expression and clinicopathological features of the invasive front in the fat infiltration area of pancreatic cancer
title_full LGR5 expression and clinicopathological features of the invasive front in the fat infiltration area of pancreatic cancer
title_fullStr LGR5 expression and clinicopathological features of the invasive front in the fat infiltration area of pancreatic cancer
title_full_unstemmed LGR5 expression and clinicopathological features of the invasive front in the fat infiltration area of pancreatic cancer
title_short LGR5 expression and clinicopathological features of the invasive front in the fat infiltration area of pancreatic cancer
title_sort lgr5 expression and clinicopathological features of the invasive front in the fat infiltration area of pancreatic cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8818226/
https://www.ncbi.nlm.nih.gov/pubmed/35123536
http://dx.doi.org/10.1186/s13000-022-01203-w
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