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Antidiarrheal and Antisecretory Effect of 80% Hydromethanolic Leaf Extract of Moringa stenopetala Baker f. in Mice

INTRODUCTION: In Ethiopia, different medicinal plants have been claimed and used to treat diarrheal diseases. However, these claimed effects for most medicinal plants have not been scientifically verified. One of such plants in Ethiopian folkloric medicine is Moringa stenopetala, which is usually co...

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Autores principales: Woldeyohannes, Menbere Getaneh, Eshete, Gelila Tamyalew, Abiye, Alfoalem Araba, Hailu, Abebe Ejigu, Huluka, Solomon Assefa, Tadesse, Wondmagegn Tamiru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8818423/
https://www.ncbi.nlm.nih.gov/pubmed/35140988
http://dx.doi.org/10.1155/2022/5768805
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author Woldeyohannes, Menbere Getaneh
Eshete, Gelila Tamyalew
Abiye, Alfoalem Araba
Hailu, Abebe Ejigu
Huluka, Solomon Assefa
Tadesse, Wondmagegn Tamiru
author_facet Woldeyohannes, Menbere Getaneh
Eshete, Gelila Tamyalew
Abiye, Alfoalem Araba
Hailu, Abebe Ejigu
Huluka, Solomon Assefa
Tadesse, Wondmagegn Tamiru
author_sort Woldeyohannes, Menbere Getaneh
collection PubMed
description INTRODUCTION: In Ethiopia, different medicinal plants have been claimed and used to treat diarrheal diseases. However, these claimed effects for most medicinal plants have not been scientifically verified. One of such plants in Ethiopian folkloric medicine is Moringa stenopetala, which is usually consumed as a vegetable in southern Ethiopia. Thus, this study aimed to evaluate the antidiarrheal and antisecretory effects of 80% methanolic leaf extract of Moringa stenopetala in different mice models. METHOD: Using Swiss albino mice, castor oil-induced diarrhea, charcoal meal-based gastrointestinal motility, and castor oil-induced secretion models were employed to assess antidiarrheal activity. In all of the test models, animals were randomly assigned into five groups consisting of six animals in each group. Group I received 0.5 ml of the vehicle (2% tween-80), while group II was treated with standard drug (3 mg/kg loperamide) in the respective models, whereas groups III to V received 150, 300, and 450 mg/kg of the methanolic leaf extracts of Moringa stenopetala. Onset, frequency, consistency, and weight of stool (diarrhea) were recorded, and different parameters and percentage proportions were calculated. Data were analyzed using one-way ANOVA followed by Tukey's test, and p < 0.05 was considered statistically significant at 95% confidence of interval. RESULT: In the castor oil-induced diarrheal model, the percentage inhibition of diarrhea was 48.5, 58.6, and 60% for the respective doses of 150, 300, and 450 mg/kg of the extract. And, the extract showed a 36.8, 54.4, and 55.9% reduction of peristalsis in charcoal meal-based gastrointestinal motility test compared to the negative control group. Moreover, in the antisecretory assay, the 150, 300, and 450 mg/kg doses of MEMS inhibited fluid contents of the stool by 11.5, 54.54, and 61.82%, respectively, relative to the vehicle-treated group. CONCLUSION: The findings revealed that the 80% methanolic leaf extract of Moringa stenopetala extract has shown antidiarrheal activity.
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spelling pubmed-88184232022-02-08 Antidiarrheal and Antisecretory Effect of 80% Hydromethanolic Leaf Extract of Moringa stenopetala Baker f. in Mice Woldeyohannes, Menbere Getaneh Eshete, Gelila Tamyalew Abiye, Alfoalem Araba Hailu, Abebe Ejigu Huluka, Solomon Assefa Tadesse, Wondmagegn Tamiru Biochem Res Int Research Article INTRODUCTION: In Ethiopia, different medicinal plants have been claimed and used to treat diarrheal diseases. However, these claimed effects for most medicinal plants have not been scientifically verified. One of such plants in Ethiopian folkloric medicine is Moringa stenopetala, which is usually consumed as a vegetable in southern Ethiopia. Thus, this study aimed to evaluate the antidiarrheal and antisecretory effects of 80% methanolic leaf extract of Moringa stenopetala in different mice models. METHOD: Using Swiss albino mice, castor oil-induced diarrhea, charcoal meal-based gastrointestinal motility, and castor oil-induced secretion models were employed to assess antidiarrheal activity. In all of the test models, animals were randomly assigned into five groups consisting of six animals in each group. Group I received 0.5 ml of the vehicle (2% tween-80), while group II was treated with standard drug (3 mg/kg loperamide) in the respective models, whereas groups III to V received 150, 300, and 450 mg/kg of the methanolic leaf extracts of Moringa stenopetala. Onset, frequency, consistency, and weight of stool (diarrhea) were recorded, and different parameters and percentage proportions were calculated. Data were analyzed using one-way ANOVA followed by Tukey's test, and p < 0.05 was considered statistically significant at 95% confidence of interval. RESULT: In the castor oil-induced diarrheal model, the percentage inhibition of diarrhea was 48.5, 58.6, and 60% for the respective doses of 150, 300, and 450 mg/kg of the extract. And, the extract showed a 36.8, 54.4, and 55.9% reduction of peristalsis in charcoal meal-based gastrointestinal motility test compared to the negative control group. Moreover, in the antisecretory assay, the 150, 300, and 450 mg/kg doses of MEMS inhibited fluid contents of the stool by 11.5, 54.54, and 61.82%, respectively, relative to the vehicle-treated group. CONCLUSION: The findings revealed that the 80% methanolic leaf extract of Moringa stenopetala extract has shown antidiarrheal activity. Hindawi 2022-01-30 /pmc/articles/PMC8818423/ /pubmed/35140988 http://dx.doi.org/10.1155/2022/5768805 Text en Copyright © 2022 Menbere Getaneh Woldeyohannes et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Woldeyohannes, Menbere Getaneh
Eshete, Gelila Tamyalew
Abiye, Alfoalem Araba
Hailu, Abebe Ejigu
Huluka, Solomon Assefa
Tadesse, Wondmagegn Tamiru
Antidiarrheal and Antisecretory Effect of 80% Hydromethanolic Leaf Extract of Moringa stenopetala Baker f. in Mice
title Antidiarrheal and Antisecretory Effect of 80% Hydromethanolic Leaf Extract of Moringa stenopetala Baker f. in Mice
title_full Antidiarrheal and Antisecretory Effect of 80% Hydromethanolic Leaf Extract of Moringa stenopetala Baker f. in Mice
title_fullStr Antidiarrheal and Antisecretory Effect of 80% Hydromethanolic Leaf Extract of Moringa stenopetala Baker f. in Mice
title_full_unstemmed Antidiarrheal and Antisecretory Effect of 80% Hydromethanolic Leaf Extract of Moringa stenopetala Baker f. in Mice
title_short Antidiarrheal and Antisecretory Effect of 80% Hydromethanolic Leaf Extract of Moringa stenopetala Baker f. in Mice
title_sort antidiarrheal and antisecretory effect of 80% hydromethanolic leaf extract of moringa stenopetala baker f. in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8818423/
https://www.ncbi.nlm.nih.gov/pubmed/35140988
http://dx.doi.org/10.1155/2022/5768805
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