Cargando…

Neuroprotective Effects of Omentin-1 Against Cerebral Hypoxia/Reoxygenation Injury via Activating GAS6/Axl Signaling Pathway in Neuroblastoma Cells

Ischemic stroke is characterized by insufficient blood supply to brain tissue and is associated with increased morbidity and mortality in adults worldwide. Growth arrest-specific protein 6 (GAS6) is a vitamin K-dependent protein and is widely expressed in the central nervous system. The biological f...

Descripción completa

Detalles Bibliográficos
Autores principales: Niu, Xiaochen, Cheng, Ye, Zhang, Meng, Du, Luyang, Wu, Xue, Lu, Chenxi, Li, Xiyang, Liu, Shuai, Zhao, Aizhen, Zhang, Shaofei, Wu, Zhen, Ding, Baoping, Shi, Wenzhen, Wang, Changyu, Yang, Yang, Tian, Ye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8818945/
https://www.ncbi.nlm.nih.gov/pubmed/35141232
http://dx.doi.org/10.3389/fcell.2021.784035
_version_ 1784645945429852160
author Niu, Xiaochen
Cheng, Ye
Zhang, Meng
Du, Luyang
Wu, Xue
Lu, Chenxi
Li, Xiyang
Liu, Shuai
Zhao, Aizhen
Zhang, Shaofei
Wu, Zhen
Ding, Baoping
Shi, Wenzhen
Wang, Changyu
Yang, Yang
Tian, Ye
author_facet Niu, Xiaochen
Cheng, Ye
Zhang, Meng
Du, Luyang
Wu, Xue
Lu, Chenxi
Li, Xiyang
Liu, Shuai
Zhao, Aizhen
Zhang, Shaofei
Wu, Zhen
Ding, Baoping
Shi, Wenzhen
Wang, Changyu
Yang, Yang
Tian, Ye
author_sort Niu, Xiaochen
collection PubMed
description Ischemic stroke is characterized by insufficient blood supply to brain tissue and is associated with increased morbidity and mortality in adults worldwide. Growth arrest-specific protein 6 (GAS6) is a vitamin K-dependent protein and is widely expressed in the central nervous system. The biological functions of GAS6 are mediated by the interaction with TAM (Tyro3, Axl and Mertk) receptors, including cell survival and proliferation, immune regulation and apoptosis. Omentin-1, also known as intelectin-1 (ITLN-1), is a novel adipocytokine that is involved in a variety of biological events, such as insulin resistance, endothelial dysfunction, programmed cell death and metabolic disorders. Our previous study has found that omentin-1 act as a novel regulator of vascular and anti-apoptotic response in cerebral ischemia. However, the specific molecular mechanism of omentin-1’s protective effect on cerebral ischemia-reperfusion injury (IRI) is still unclear. First, the toxicity of recombinant human omentin-1 (rh-omentin) was assessed and a safe concentration was chosen for the next experiments. Then, rh-omentin exerted neuroprotection against hypoxia/reoxygenation (H/R) injury in N2a cells, indicated by increased cell viability, decreased LDH, ROS generation, and cell apoptotic rate. Furthermore, the similar protective effect was observed in omentin-1 overexpression cells constructed by lentivirus transfection. Rh-omentin could also inhibit H/R-induced apoptotic molecules, oxidative stress molecules, and GAS6/Axl signaling molecules which as evidence by increased omentin-1, GAS6, Axl, p-Axl, NQO1, HO-1, Nrf2, Bcl2 and decreased Bax expressions. However, GAS6 siRNA could reverse rh-omentin-induced neuroprotection and the levels of these molecules mentioned above. In conclusion, these findings suggest that omentin-1 treatment exerts neuroprotection against H/R injury partly via activating GAS6/Axl signaling at least. Therefore, these finding may favor omentin-1 a potential neuroprotective drug candidate to alleviate ischemia-reperfusion injury in clinic.
format Online
Article
Text
id pubmed-8818945
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88189452022-02-08 Neuroprotective Effects of Omentin-1 Against Cerebral Hypoxia/Reoxygenation Injury via Activating GAS6/Axl Signaling Pathway in Neuroblastoma Cells Niu, Xiaochen Cheng, Ye Zhang, Meng Du, Luyang Wu, Xue Lu, Chenxi Li, Xiyang Liu, Shuai Zhao, Aizhen Zhang, Shaofei Wu, Zhen Ding, Baoping Shi, Wenzhen Wang, Changyu Yang, Yang Tian, Ye Front Cell Dev Biol Cell and Developmental Biology Ischemic stroke is characterized by insufficient blood supply to brain tissue and is associated with increased morbidity and mortality in adults worldwide. Growth arrest-specific protein 6 (GAS6) is a vitamin K-dependent protein and is widely expressed in the central nervous system. The biological functions of GAS6 are mediated by the interaction with TAM (Tyro3, Axl and Mertk) receptors, including cell survival and proliferation, immune regulation and apoptosis. Omentin-1, also known as intelectin-1 (ITLN-1), is a novel adipocytokine that is involved in a variety of biological events, such as insulin resistance, endothelial dysfunction, programmed cell death and metabolic disorders. Our previous study has found that omentin-1 act as a novel regulator of vascular and anti-apoptotic response in cerebral ischemia. However, the specific molecular mechanism of omentin-1’s protective effect on cerebral ischemia-reperfusion injury (IRI) is still unclear. First, the toxicity of recombinant human omentin-1 (rh-omentin) was assessed and a safe concentration was chosen for the next experiments. Then, rh-omentin exerted neuroprotection against hypoxia/reoxygenation (H/R) injury in N2a cells, indicated by increased cell viability, decreased LDH, ROS generation, and cell apoptotic rate. Furthermore, the similar protective effect was observed in omentin-1 overexpression cells constructed by lentivirus transfection. Rh-omentin could also inhibit H/R-induced apoptotic molecules, oxidative stress molecules, and GAS6/Axl signaling molecules which as evidence by increased omentin-1, GAS6, Axl, p-Axl, NQO1, HO-1, Nrf2, Bcl2 and decreased Bax expressions. However, GAS6 siRNA could reverse rh-omentin-induced neuroprotection and the levels of these molecules mentioned above. In conclusion, these findings suggest that omentin-1 treatment exerts neuroprotection against H/R injury partly via activating GAS6/Axl signaling at least. Therefore, these finding may favor omentin-1 a potential neuroprotective drug candidate to alleviate ischemia-reperfusion injury in clinic. Frontiers Media S.A. 2022-01-24 /pmc/articles/PMC8818945/ /pubmed/35141232 http://dx.doi.org/10.3389/fcell.2021.784035 Text en Copyright © 2022 Niu, Cheng, Zhang, Du, Wu, Lu, Li, Liu, Zhao, Zhang, Wu, Ding, Shi, Wang, Yang and Tian. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Niu, Xiaochen
Cheng, Ye
Zhang, Meng
Du, Luyang
Wu, Xue
Lu, Chenxi
Li, Xiyang
Liu, Shuai
Zhao, Aizhen
Zhang, Shaofei
Wu, Zhen
Ding, Baoping
Shi, Wenzhen
Wang, Changyu
Yang, Yang
Tian, Ye
Neuroprotective Effects of Omentin-1 Against Cerebral Hypoxia/Reoxygenation Injury via Activating GAS6/Axl Signaling Pathway in Neuroblastoma Cells
title Neuroprotective Effects of Omentin-1 Against Cerebral Hypoxia/Reoxygenation Injury via Activating GAS6/Axl Signaling Pathway in Neuroblastoma Cells
title_full Neuroprotective Effects of Omentin-1 Against Cerebral Hypoxia/Reoxygenation Injury via Activating GAS6/Axl Signaling Pathway in Neuroblastoma Cells
title_fullStr Neuroprotective Effects of Omentin-1 Against Cerebral Hypoxia/Reoxygenation Injury via Activating GAS6/Axl Signaling Pathway in Neuroblastoma Cells
title_full_unstemmed Neuroprotective Effects of Omentin-1 Against Cerebral Hypoxia/Reoxygenation Injury via Activating GAS6/Axl Signaling Pathway in Neuroblastoma Cells
title_short Neuroprotective Effects of Omentin-1 Against Cerebral Hypoxia/Reoxygenation Injury via Activating GAS6/Axl Signaling Pathway in Neuroblastoma Cells
title_sort neuroprotective effects of omentin-1 against cerebral hypoxia/reoxygenation injury via activating gas6/axl signaling pathway in neuroblastoma cells
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8818945/
https://www.ncbi.nlm.nih.gov/pubmed/35141232
http://dx.doi.org/10.3389/fcell.2021.784035
work_keys_str_mv AT niuxiaochen neuroprotectiveeffectsofomentin1againstcerebralhypoxiareoxygenationinjuryviaactivatinggas6axlsignalingpathwayinneuroblastomacells
AT chengye neuroprotectiveeffectsofomentin1againstcerebralhypoxiareoxygenationinjuryviaactivatinggas6axlsignalingpathwayinneuroblastomacells
AT zhangmeng neuroprotectiveeffectsofomentin1againstcerebralhypoxiareoxygenationinjuryviaactivatinggas6axlsignalingpathwayinneuroblastomacells
AT duluyang neuroprotectiveeffectsofomentin1againstcerebralhypoxiareoxygenationinjuryviaactivatinggas6axlsignalingpathwayinneuroblastomacells
AT wuxue neuroprotectiveeffectsofomentin1againstcerebralhypoxiareoxygenationinjuryviaactivatinggas6axlsignalingpathwayinneuroblastomacells
AT luchenxi neuroprotectiveeffectsofomentin1againstcerebralhypoxiareoxygenationinjuryviaactivatinggas6axlsignalingpathwayinneuroblastomacells
AT lixiyang neuroprotectiveeffectsofomentin1againstcerebralhypoxiareoxygenationinjuryviaactivatinggas6axlsignalingpathwayinneuroblastomacells
AT liushuai neuroprotectiveeffectsofomentin1againstcerebralhypoxiareoxygenationinjuryviaactivatinggas6axlsignalingpathwayinneuroblastomacells
AT zhaoaizhen neuroprotectiveeffectsofomentin1againstcerebralhypoxiareoxygenationinjuryviaactivatinggas6axlsignalingpathwayinneuroblastomacells
AT zhangshaofei neuroprotectiveeffectsofomentin1againstcerebralhypoxiareoxygenationinjuryviaactivatinggas6axlsignalingpathwayinneuroblastomacells
AT wuzhen neuroprotectiveeffectsofomentin1againstcerebralhypoxiareoxygenationinjuryviaactivatinggas6axlsignalingpathwayinneuroblastomacells
AT dingbaoping neuroprotectiveeffectsofomentin1againstcerebralhypoxiareoxygenationinjuryviaactivatinggas6axlsignalingpathwayinneuroblastomacells
AT shiwenzhen neuroprotectiveeffectsofomentin1againstcerebralhypoxiareoxygenationinjuryviaactivatinggas6axlsignalingpathwayinneuroblastomacells
AT wangchangyu neuroprotectiveeffectsofomentin1againstcerebralhypoxiareoxygenationinjuryviaactivatinggas6axlsignalingpathwayinneuroblastomacells
AT yangyang neuroprotectiveeffectsofomentin1againstcerebralhypoxiareoxygenationinjuryviaactivatinggas6axlsignalingpathwayinneuroblastomacells
AT tianye neuroprotectiveeffectsofomentin1againstcerebralhypoxiareoxygenationinjuryviaactivatinggas6axlsignalingpathwayinneuroblastomacells