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Seven Genes Associated With Lymphatic Metastasis in Thyroid Cancer That Is Linked to Tumor Immune Cell Infiltration

OBJECTIVE: Since there are few studies exploring genes associated with lymphatic metastasis of thyroid carcinoma (THCA), this study was conducted to explore genes associated with lymphatic metastasis of THCA and to investigate the relationship with immune infiltration. METHODS: Differentially expres...

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Autores principales: Wu, Linfeng, Zhou, Yuying, Guan, Yaoyao, Xiao, Rongyao, Cai, Jiaohao, Chen, Weike, Zheng, Mengmeng, Sun, Kaiting, Chen, Chao, Huang, Guanli, Zhang, Xiaogang, Qian, Ziliang, Shen, Shurong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8818997/
https://www.ncbi.nlm.nih.gov/pubmed/35141140
http://dx.doi.org/10.3389/fonc.2021.756246
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author Wu, Linfeng
Zhou, Yuying
Guan, Yaoyao
Xiao, Rongyao
Cai, Jiaohao
Chen, Weike
Zheng, Mengmeng
Sun, Kaiting
Chen, Chao
Huang, Guanli
Zhang, Xiaogang
Qian, Ziliang
Shen, Shurong
author_facet Wu, Linfeng
Zhou, Yuying
Guan, Yaoyao
Xiao, Rongyao
Cai, Jiaohao
Chen, Weike
Zheng, Mengmeng
Sun, Kaiting
Chen, Chao
Huang, Guanli
Zhang, Xiaogang
Qian, Ziliang
Shen, Shurong
author_sort Wu, Linfeng
collection PubMed
description OBJECTIVE: Since there are few studies exploring genes associated with lymphatic metastasis of thyroid carcinoma (THCA), this study was conducted to explore genes associated with lymphatic metastasis of THCA and to investigate the relationship with immune infiltration. METHODS: Differentially expressed genes associated with THCA lymphatic metastasis were analyzed based on The Cancer Genome Atlas Program (TCGA) database; a protein-protein interaction(PPI)network was constructed to screen for pivotal genes. Based on the identified hub genes, their expression in THCA with and without lymphatic metastasis were determined. Functional enrichment analysis was performed. The correlation between the identified genes and immune cell infiltration was explored. LASSO logistic regression analysis was performed to determine the risk score of the most relevant gene constructs and multifactor COX regression analysis based on genes in the risk score formula. RESULTS: A total of 115 genes were differentially expressed in THCA with and without lymphatic metastasis, including 28 upregulated genes and 87 downregulated genes. The PPI network identified seven hub genes (EVA1A, TIMP1, SERPINA1, FAM20A, FN1, TNC, MXRA8); the expression of all seven genes was upregulated in the group with lymphatic metastasis; Immuno-infiltration analysis showed that all seven genes were significantly positively correlated with macrophage M1 and NK cells and negatively correlated with T-cell CD4+ and myeloid dendritic cells. LASSO logistic regression analysis identified the five most relevant genes (EVA1A, SERPINA1, FN1, TNC, MXRA8), and multi-factor COX regression analysis showed EVA1A, SERPINA1 and FN1 as independent prognostic factors. CONCLUSION: Seven genes were associated with lymphatic metastasis of THCA and with tumor immune cell infiltration.
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spelling pubmed-88189972022-02-08 Seven Genes Associated With Lymphatic Metastasis in Thyroid Cancer That Is Linked to Tumor Immune Cell Infiltration Wu, Linfeng Zhou, Yuying Guan, Yaoyao Xiao, Rongyao Cai, Jiaohao Chen, Weike Zheng, Mengmeng Sun, Kaiting Chen, Chao Huang, Guanli Zhang, Xiaogang Qian, Ziliang Shen, Shurong Front Oncol Oncology OBJECTIVE: Since there are few studies exploring genes associated with lymphatic metastasis of thyroid carcinoma (THCA), this study was conducted to explore genes associated with lymphatic metastasis of THCA and to investigate the relationship with immune infiltration. METHODS: Differentially expressed genes associated with THCA lymphatic metastasis were analyzed based on The Cancer Genome Atlas Program (TCGA) database; a protein-protein interaction(PPI)network was constructed to screen for pivotal genes. Based on the identified hub genes, their expression in THCA with and without lymphatic metastasis were determined. Functional enrichment analysis was performed. The correlation between the identified genes and immune cell infiltration was explored. LASSO logistic regression analysis was performed to determine the risk score of the most relevant gene constructs and multifactor COX regression analysis based on genes in the risk score formula. RESULTS: A total of 115 genes were differentially expressed in THCA with and without lymphatic metastasis, including 28 upregulated genes and 87 downregulated genes. The PPI network identified seven hub genes (EVA1A, TIMP1, SERPINA1, FAM20A, FN1, TNC, MXRA8); the expression of all seven genes was upregulated in the group with lymphatic metastasis; Immuno-infiltration analysis showed that all seven genes were significantly positively correlated with macrophage M1 and NK cells and negatively correlated with T-cell CD4+ and myeloid dendritic cells. LASSO logistic regression analysis identified the five most relevant genes (EVA1A, SERPINA1, FN1, TNC, MXRA8), and multi-factor COX regression analysis showed EVA1A, SERPINA1 and FN1 as independent prognostic factors. CONCLUSION: Seven genes were associated with lymphatic metastasis of THCA and with tumor immune cell infiltration. Frontiers Media S.A. 2022-01-24 /pmc/articles/PMC8818997/ /pubmed/35141140 http://dx.doi.org/10.3389/fonc.2021.756246 Text en Copyright © 2022 Wu, Zhou, Guan, Xiao, Cai, Chen, Zheng, Sun, Chen, Huang, Zhang, Qian and Shen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Wu, Linfeng
Zhou, Yuying
Guan, Yaoyao
Xiao, Rongyao
Cai, Jiaohao
Chen, Weike
Zheng, Mengmeng
Sun, Kaiting
Chen, Chao
Huang, Guanli
Zhang, Xiaogang
Qian, Ziliang
Shen, Shurong
Seven Genes Associated With Lymphatic Metastasis in Thyroid Cancer That Is Linked to Tumor Immune Cell Infiltration
title Seven Genes Associated With Lymphatic Metastasis in Thyroid Cancer That Is Linked to Tumor Immune Cell Infiltration
title_full Seven Genes Associated With Lymphatic Metastasis in Thyroid Cancer That Is Linked to Tumor Immune Cell Infiltration
title_fullStr Seven Genes Associated With Lymphatic Metastasis in Thyroid Cancer That Is Linked to Tumor Immune Cell Infiltration
title_full_unstemmed Seven Genes Associated With Lymphatic Metastasis in Thyroid Cancer That Is Linked to Tumor Immune Cell Infiltration
title_short Seven Genes Associated With Lymphatic Metastasis in Thyroid Cancer That Is Linked to Tumor Immune Cell Infiltration
title_sort seven genes associated with lymphatic metastasis in thyroid cancer that is linked to tumor immune cell infiltration
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8818997/
https://www.ncbi.nlm.nih.gov/pubmed/35141140
http://dx.doi.org/10.3389/fonc.2021.756246
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