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In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina

The present study challenges the in vivo assessment of cold atmospheric pressure plasma (CAPP) technology on the bioactive activity (antioxidant/antiaging and antimicrobial potential) of Spirulina powder, using Caenorhabditis elegans as an animal model. Surface microdischarge cold atmospheric pressu...

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Autores principales: Pina-Pérez, María Consuelo, Úbeda-Manzanaro, María, Beyrer, Michael, Martínez, Antonio, Rodrigo, Dolores
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8819064/
https://www.ncbi.nlm.nih.gov/pubmed/35140692
http://dx.doi.org/10.3389/fmicb.2021.781871
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author Pina-Pérez, María Consuelo
Úbeda-Manzanaro, María
Beyrer, Michael
Martínez, Antonio
Rodrigo, Dolores
author_facet Pina-Pérez, María Consuelo
Úbeda-Manzanaro, María
Beyrer, Michael
Martínez, Antonio
Rodrigo, Dolores
author_sort Pina-Pérez, María Consuelo
collection PubMed
description The present study challenges the in vivo assessment of cold atmospheric pressure plasma (CAPP) technology on the bioactive activity (antioxidant/antiaging and antimicrobial potential) of Spirulina powder, using Caenorhabditis elegans as an animal model. Surface microdischarge cold atmospheric pressure plasma (SMD-CAPP) treatment was 3.3 W discharge power for 7 min. C. elegans lifespan and egg laying were used as indicators of antioxidant/antiaging potential of Spirulina (1 mg/mL), when grown with Spirulina CP-treated [E_SCP] and untreated [E_S], compared with a control [E_0] (non-supplemented with Spirulina). According to our results, under both Spirulina supplemented media [E_SCP and E_S] and for the first 17 days, nematodes experienced an increase in lifespan but without significant differences (p > 0.05) between control and Spirulina CP-treated. Regarding the in vivo assay of the antimicrobial potential of Spirulina against Salmonella enterica serovar Typhimurium (infected worms), no significant differences (p > 0.05) were found between the three exposure scenarios (control [S_0]; Spirulina supplemented media [S_S]; CP-treated Spirulina supplemented media [S_SCP]). According to present results, CAPP-treatment do not influence negatively the lifespan of C. elegans but a reduction in the Spirulina antiaging potential was found. No in vivo modifications in antimicrobial activity seem to be linked to CAPP-processed Spirulina.
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spelling pubmed-88190642022-02-08 In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina Pina-Pérez, María Consuelo Úbeda-Manzanaro, María Beyrer, Michael Martínez, Antonio Rodrigo, Dolores Front Microbiol Microbiology The present study challenges the in vivo assessment of cold atmospheric pressure plasma (CAPP) technology on the bioactive activity (antioxidant/antiaging and antimicrobial potential) of Spirulina powder, using Caenorhabditis elegans as an animal model. Surface microdischarge cold atmospheric pressure plasma (SMD-CAPP) treatment was 3.3 W discharge power for 7 min. C. elegans lifespan and egg laying were used as indicators of antioxidant/antiaging potential of Spirulina (1 mg/mL), when grown with Spirulina CP-treated [E_SCP] and untreated [E_S], compared with a control [E_0] (non-supplemented with Spirulina). According to our results, under both Spirulina supplemented media [E_SCP and E_S] and for the first 17 days, nematodes experienced an increase in lifespan but without significant differences (p > 0.05) between control and Spirulina CP-treated. Regarding the in vivo assay of the antimicrobial potential of Spirulina against Salmonella enterica serovar Typhimurium (infected worms), no significant differences (p > 0.05) were found between the three exposure scenarios (control [S_0]; Spirulina supplemented media [S_S]; CP-treated Spirulina supplemented media [S_SCP]). According to present results, CAPP-treatment do not influence negatively the lifespan of C. elegans but a reduction in the Spirulina antiaging potential was found. No in vivo modifications in antimicrobial activity seem to be linked to CAPP-processed Spirulina. Frontiers Media S.A. 2022-01-24 /pmc/articles/PMC8819064/ /pubmed/35140692 http://dx.doi.org/10.3389/fmicb.2021.781871 Text en Copyright © 2022 Pina-Pérez, Úbeda-Manzanaro, Beyrer, Martínez and Rodrigo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Pina-Pérez, María Consuelo
Úbeda-Manzanaro, María
Beyrer, Michael
Martínez, Antonio
Rodrigo, Dolores
In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina
title In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina
title_full In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina
title_fullStr In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina
title_full_unstemmed In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina
title_short In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina
title_sort in vivo assessment of cold atmospheric pressure plasma technology on the bioactivity of spirulina
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8819064/
https://www.ncbi.nlm.nih.gov/pubmed/35140692
http://dx.doi.org/10.3389/fmicb.2021.781871
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