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In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina
The present study challenges the in vivo assessment of cold atmospheric pressure plasma (CAPP) technology on the bioactive activity (antioxidant/antiaging and antimicrobial potential) of Spirulina powder, using Caenorhabditis elegans as an animal model. Surface microdischarge cold atmospheric pressu...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8819064/ https://www.ncbi.nlm.nih.gov/pubmed/35140692 http://dx.doi.org/10.3389/fmicb.2021.781871 |
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author | Pina-Pérez, María Consuelo Úbeda-Manzanaro, María Beyrer, Michael Martínez, Antonio Rodrigo, Dolores |
author_facet | Pina-Pérez, María Consuelo Úbeda-Manzanaro, María Beyrer, Michael Martínez, Antonio Rodrigo, Dolores |
author_sort | Pina-Pérez, María Consuelo |
collection | PubMed |
description | The present study challenges the in vivo assessment of cold atmospheric pressure plasma (CAPP) technology on the bioactive activity (antioxidant/antiaging and antimicrobial potential) of Spirulina powder, using Caenorhabditis elegans as an animal model. Surface microdischarge cold atmospheric pressure plasma (SMD-CAPP) treatment was 3.3 W discharge power for 7 min. C. elegans lifespan and egg laying were used as indicators of antioxidant/antiaging potential of Spirulina (1 mg/mL), when grown with Spirulina CP-treated [E_SCP] and untreated [E_S], compared with a control [E_0] (non-supplemented with Spirulina). According to our results, under both Spirulina supplemented media [E_SCP and E_S] and for the first 17 days, nematodes experienced an increase in lifespan but without significant differences (p > 0.05) between control and Spirulina CP-treated. Regarding the in vivo assay of the antimicrobial potential of Spirulina against Salmonella enterica serovar Typhimurium (infected worms), no significant differences (p > 0.05) were found between the three exposure scenarios (control [S_0]; Spirulina supplemented media [S_S]; CP-treated Spirulina supplemented media [S_SCP]). According to present results, CAPP-treatment do not influence negatively the lifespan of C. elegans but a reduction in the Spirulina antiaging potential was found. No in vivo modifications in antimicrobial activity seem to be linked to CAPP-processed Spirulina. |
format | Online Article Text |
id | pubmed-8819064 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88190642022-02-08 In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina Pina-Pérez, María Consuelo Úbeda-Manzanaro, María Beyrer, Michael Martínez, Antonio Rodrigo, Dolores Front Microbiol Microbiology The present study challenges the in vivo assessment of cold atmospheric pressure plasma (CAPP) technology on the bioactive activity (antioxidant/antiaging and antimicrobial potential) of Spirulina powder, using Caenorhabditis elegans as an animal model. Surface microdischarge cold atmospheric pressure plasma (SMD-CAPP) treatment was 3.3 W discharge power for 7 min. C. elegans lifespan and egg laying were used as indicators of antioxidant/antiaging potential of Spirulina (1 mg/mL), when grown with Spirulina CP-treated [E_SCP] and untreated [E_S], compared with a control [E_0] (non-supplemented with Spirulina). According to our results, under both Spirulina supplemented media [E_SCP and E_S] and for the first 17 days, nematodes experienced an increase in lifespan but without significant differences (p > 0.05) between control and Spirulina CP-treated. Regarding the in vivo assay of the antimicrobial potential of Spirulina against Salmonella enterica serovar Typhimurium (infected worms), no significant differences (p > 0.05) were found between the three exposure scenarios (control [S_0]; Spirulina supplemented media [S_S]; CP-treated Spirulina supplemented media [S_SCP]). According to present results, CAPP-treatment do not influence negatively the lifespan of C. elegans but a reduction in the Spirulina antiaging potential was found. No in vivo modifications in antimicrobial activity seem to be linked to CAPP-processed Spirulina. Frontiers Media S.A. 2022-01-24 /pmc/articles/PMC8819064/ /pubmed/35140692 http://dx.doi.org/10.3389/fmicb.2021.781871 Text en Copyright © 2022 Pina-Pérez, Úbeda-Manzanaro, Beyrer, Martínez and Rodrigo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Pina-Pérez, María Consuelo Úbeda-Manzanaro, María Beyrer, Michael Martínez, Antonio Rodrigo, Dolores In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina |
title | In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina |
title_full | In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina |
title_fullStr | In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina |
title_full_unstemmed | In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina |
title_short | In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina |
title_sort | in vivo assessment of cold atmospheric pressure plasma technology on the bioactivity of spirulina |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8819064/ https://www.ncbi.nlm.nih.gov/pubmed/35140692 http://dx.doi.org/10.3389/fmicb.2021.781871 |
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