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Antigen Discovery in Circulating Extracellular Vesicles From Plasmodium vivax Patients
Plasmodium vivax is the most widely distributed human malaria parasite with 7 million annual clinical cases and 2.5 billion people living under risk of infection. There is an urgent need to discover new antigens for vaccination as only two vaccine candidates are currently in clinical trials. Extrace...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8819181/ https://www.ncbi.nlm.nih.gov/pubmed/35141172 http://dx.doi.org/10.3389/fcimb.2021.811390 |
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author | Aparici-Herraiz, Iris Gualdrón-López, Melisa Castro-Cavadía, Carlos J. Carmona-Fonseca, Jaime Yasnot, María Fernanda Fernandez-Becerra, Carmen del Portillo, Hernando A. |
author_facet | Aparici-Herraiz, Iris Gualdrón-López, Melisa Castro-Cavadía, Carlos J. Carmona-Fonseca, Jaime Yasnot, María Fernanda Fernandez-Becerra, Carmen del Portillo, Hernando A. |
author_sort | Aparici-Herraiz, Iris |
collection | PubMed |
description | Plasmodium vivax is the most widely distributed human malaria parasite with 7 million annual clinical cases and 2.5 billion people living under risk of infection. There is an urgent need to discover new antigens for vaccination as only two vaccine candidates are currently in clinical trials. Extracellular vesicles (EVs) are small membrane-bound vesicles involved in intercellular communication and initially described in reticulocytes, the host cell of P. vivax, as a selective disposal mechanism of the transferrin receptor (CD71) in the maturation of reticulocytes to erythrocytes. We have recently reported the proteomics identification of P. vivax proteins associated to circulating EVs in P. vivax patients using size exclusion chromatography followed by mass spectrometry (MS). Parasite proteins were detected in only two out of ten patients. To increase the MS signal, we have implemented the direct immuno-affinity capture (DIC) technique to enrich in EVs derived from CD71-expressing cells. Remarkably, we identified parasite proteins in all patients totaling 48 proteins and including several previously identified P. vivax vaccine candidate antigens (MSP1, MSP3, MSP7, MSP9, Serine-repeat antigen 1, and HSP70) as well as membrane, cytosolic and exported proteins. Notably, a member of the Plasmodium helical interspersed sub-telomeric (PHIST-c) family and a member of the Plasmodium exported proteins, were detected in five out of six analyzed patients. Humoral immune response analysis using sera from vivax patients confirmed the antigenicity of the PHIST-c protein. Collectively, we showed that enrichment of EVs by CD71-DIC from plasma of patients, allows a robust identification of P. vivax immunogenic proteins. This study represents a significant advance in identifying new antigens for vaccination against this human malaria parasite. |
format | Online Article Text |
id | pubmed-8819181 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88191812022-02-08 Antigen Discovery in Circulating Extracellular Vesicles From Plasmodium vivax Patients Aparici-Herraiz, Iris Gualdrón-López, Melisa Castro-Cavadía, Carlos J. Carmona-Fonseca, Jaime Yasnot, María Fernanda Fernandez-Becerra, Carmen del Portillo, Hernando A. Front Cell Infect Microbiol Cellular and Infection Microbiology Plasmodium vivax is the most widely distributed human malaria parasite with 7 million annual clinical cases and 2.5 billion people living under risk of infection. There is an urgent need to discover new antigens for vaccination as only two vaccine candidates are currently in clinical trials. Extracellular vesicles (EVs) are small membrane-bound vesicles involved in intercellular communication and initially described in reticulocytes, the host cell of P. vivax, as a selective disposal mechanism of the transferrin receptor (CD71) in the maturation of reticulocytes to erythrocytes. We have recently reported the proteomics identification of P. vivax proteins associated to circulating EVs in P. vivax patients using size exclusion chromatography followed by mass spectrometry (MS). Parasite proteins were detected in only two out of ten patients. To increase the MS signal, we have implemented the direct immuno-affinity capture (DIC) technique to enrich in EVs derived from CD71-expressing cells. Remarkably, we identified parasite proteins in all patients totaling 48 proteins and including several previously identified P. vivax vaccine candidate antigens (MSP1, MSP3, MSP7, MSP9, Serine-repeat antigen 1, and HSP70) as well as membrane, cytosolic and exported proteins. Notably, a member of the Plasmodium helical interspersed sub-telomeric (PHIST-c) family and a member of the Plasmodium exported proteins, were detected in five out of six analyzed patients. Humoral immune response analysis using sera from vivax patients confirmed the antigenicity of the PHIST-c protein. Collectively, we showed that enrichment of EVs by CD71-DIC from plasma of patients, allows a robust identification of P. vivax immunogenic proteins. This study represents a significant advance in identifying new antigens for vaccination against this human malaria parasite. Frontiers Media S.A. 2022-01-24 /pmc/articles/PMC8819181/ /pubmed/35141172 http://dx.doi.org/10.3389/fcimb.2021.811390 Text en Copyright © 2022 Aparici-Herraiz, Gualdrón-López, Castro-Cavadía, Carmona-Fonseca, Yasnot, Fernandez-Becerra and del Portillo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Aparici-Herraiz, Iris Gualdrón-López, Melisa Castro-Cavadía, Carlos J. Carmona-Fonseca, Jaime Yasnot, María Fernanda Fernandez-Becerra, Carmen del Portillo, Hernando A. Antigen Discovery in Circulating Extracellular Vesicles From Plasmodium vivax Patients |
title | Antigen Discovery in Circulating Extracellular Vesicles From Plasmodium vivax Patients |
title_full | Antigen Discovery in Circulating Extracellular Vesicles From Plasmodium vivax Patients |
title_fullStr | Antigen Discovery in Circulating Extracellular Vesicles From Plasmodium vivax Patients |
title_full_unstemmed | Antigen Discovery in Circulating Extracellular Vesicles From Plasmodium vivax Patients |
title_short | Antigen Discovery in Circulating Extracellular Vesicles From Plasmodium vivax Patients |
title_sort | antigen discovery in circulating extracellular vesicles from plasmodium vivax patients |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8819181/ https://www.ncbi.nlm.nih.gov/pubmed/35141172 http://dx.doi.org/10.3389/fcimb.2021.811390 |
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