Isolation of myenteric and submucosal plexus from mouse gastrointestinal tract and subsequent flow cytometry and immunofluorescence

The myenteric plexus is located between the longitudinal and circular layers of muscularis externa in the gastrointestinal tract. It contains a large network of enteric neurons that form the enteric nervous system (ENS) and control intestinal functions, such as motility and nutrient sensing. This pr...

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Detalles Bibliográficos
Autores principales: Ahrends, Tomasz, Weiner, Molly, Mucida, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8819479/
https://www.ncbi.nlm.nih.gov/pubmed/35146454
http://dx.doi.org/10.1016/j.xpro.2022.101157
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author Ahrends, Tomasz
Weiner, Molly
Mucida, Daniel
author_facet Ahrends, Tomasz
Weiner, Molly
Mucida, Daniel
author_sort Ahrends, Tomasz
collection PubMed
description The myenteric plexus is located between the longitudinal and circular layers of muscularis externa in the gastrointestinal tract. It contains a large network of enteric neurons that form the enteric nervous system (ENS) and control intestinal functions, such as motility and nutrient sensing. This protocol describes the method for physical separation (peeling) of muscularis and submucosal layers of the mouse intestine. Subsequently, the intestinal layers are then processed for flow cytometry and/or immunofluorescence analysis. For complete details on the use and execution of this profile, please refer to Ahrends et al. (2021).
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spelling pubmed-88194792022-02-09 Isolation of myenteric and submucosal plexus from mouse gastrointestinal tract and subsequent flow cytometry and immunofluorescence Ahrends, Tomasz Weiner, Molly Mucida, Daniel STAR Protoc Protocol The myenteric plexus is located between the longitudinal and circular layers of muscularis externa in the gastrointestinal tract. It contains a large network of enteric neurons that form the enteric nervous system (ENS) and control intestinal functions, such as motility and nutrient sensing. This protocol describes the method for physical separation (peeling) of muscularis and submucosal layers of the mouse intestine. Subsequently, the intestinal layers are then processed for flow cytometry and/or immunofluorescence analysis. For complete details on the use and execution of this profile, please refer to Ahrends et al. (2021). Elsevier 2022-01-20 /pmc/articles/PMC8819479/ /pubmed/35146454 http://dx.doi.org/10.1016/j.xpro.2022.101157 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ahrends, Tomasz
Weiner, Molly
Mucida, Daniel
Isolation of myenteric and submucosal plexus from mouse gastrointestinal tract and subsequent flow cytometry and immunofluorescence
title Isolation of myenteric and submucosal plexus from mouse gastrointestinal tract and subsequent flow cytometry and immunofluorescence
title_full Isolation of myenteric and submucosal plexus from mouse gastrointestinal tract and subsequent flow cytometry and immunofluorescence
title_fullStr Isolation of myenteric and submucosal plexus from mouse gastrointestinal tract and subsequent flow cytometry and immunofluorescence
title_full_unstemmed Isolation of myenteric and submucosal plexus from mouse gastrointestinal tract and subsequent flow cytometry and immunofluorescence
title_short Isolation of myenteric and submucosal plexus from mouse gastrointestinal tract and subsequent flow cytometry and immunofluorescence
title_sort isolation of myenteric and submucosal plexus from mouse gastrointestinal tract and subsequent flow cytometry and immunofluorescence
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8819479/
https://www.ncbi.nlm.nih.gov/pubmed/35146454
http://dx.doi.org/10.1016/j.xpro.2022.101157
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