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Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons
Peripheral nerve fibroblasts play a critical role in nerve development and regeneration. Our previous study found that peripheral nerve fibroblasts have different sensory and motor phenotypes. Fibroblasts of different phenotypes can guide the migration of Schwann cells to the same sensory or motor p...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8820717/ https://www.ncbi.nlm.nih.gov/pubmed/35017446 http://dx.doi.org/10.4103/1673-5374.332159 |
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author | He, Qian-Ru Cong, Meng Yu, Fan-Hui Ji, Yu-Hua Yu, Shu Shi, Hai-Yan Ding, Fei |
author_facet | He, Qian-Ru Cong, Meng Yu, Fan-Hui Ji, Yu-Hua Yu, Shu Shi, Hai-Yan Ding, Fei |
author_sort | He, Qian-Ru |
collection | PubMed |
description | Peripheral nerve fibroblasts play a critical role in nerve development and regeneration. Our previous study found that peripheral nerve fibroblasts have different sensory and motor phenotypes. Fibroblasts of different phenotypes can guide the migration of Schwann cells to the same sensory or motor phenotype. In this study, we analyzed the different effects of peripheral nerve-derived fibroblasts and cardiac fibroblasts on motoneurons. Compared with cardiac fibroblasts, peripheral nerve fibroblasts greatly promoted motoneuron neurite outgrowth. Transcriptome analysis results identified 491 genes that were differentially expressed in peripheral nerve fibroblasts and cardiac fibroblasts. Among these, 130 were significantly upregulated in peripheral nerve fibroblasts compared with cardiac fibroblasts. These genes may be involved in axon guidance and neuron projection. Three days after sciatic nerve transection in rats, peripheral nerve fibroblasts accumulated in the proximal and distal nerve stumps, and most expressed brain-derived neurotrophic factor. In vitro, brain-derived neurotrophic factor secreted from peripheral nerve fibroblasts increased the expression of β-actin and F-actin through the extracellular regulated protein kinase and serine/threonine kinase pathways, and enhanced motoneuron neurite outgrowth. These findings suggest that peripheral nerve fibroblasts and cardiac fibroblasts exhibit different patterns of gene expression. Peripheral nerve fibroblasts can promote motoneuron neurite outgrowth. |
format | Online Article Text |
id | pubmed-8820717 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-88207172022-02-24 Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons He, Qian-Ru Cong, Meng Yu, Fan-Hui Ji, Yu-Hua Yu, Shu Shi, Hai-Yan Ding, Fei Neural Regen Res Research Article Peripheral nerve fibroblasts play a critical role in nerve development and regeneration. Our previous study found that peripheral nerve fibroblasts have different sensory and motor phenotypes. Fibroblasts of different phenotypes can guide the migration of Schwann cells to the same sensory or motor phenotype. In this study, we analyzed the different effects of peripheral nerve-derived fibroblasts and cardiac fibroblasts on motoneurons. Compared with cardiac fibroblasts, peripheral nerve fibroblasts greatly promoted motoneuron neurite outgrowth. Transcriptome analysis results identified 491 genes that were differentially expressed in peripheral nerve fibroblasts and cardiac fibroblasts. Among these, 130 were significantly upregulated in peripheral nerve fibroblasts compared with cardiac fibroblasts. These genes may be involved in axon guidance and neuron projection. Three days after sciatic nerve transection in rats, peripheral nerve fibroblasts accumulated in the proximal and distal nerve stumps, and most expressed brain-derived neurotrophic factor. In vitro, brain-derived neurotrophic factor secreted from peripheral nerve fibroblasts increased the expression of β-actin and F-actin through the extracellular regulated protein kinase and serine/threonine kinase pathways, and enhanced motoneuron neurite outgrowth. These findings suggest that peripheral nerve fibroblasts and cardiac fibroblasts exhibit different patterns of gene expression. Peripheral nerve fibroblasts can promote motoneuron neurite outgrowth. Wolters Kluwer - Medknow 2022-01-07 /pmc/articles/PMC8820717/ /pubmed/35017446 http://dx.doi.org/10.4103/1673-5374.332159 Text en Copyright: © Neural Regeneration Research https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Research Article He, Qian-Ru Cong, Meng Yu, Fan-Hui Ji, Yu-Hua Yu, Shu Shi, Hai-Yan Ding, Fei Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons |
title | Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons |
title_full | Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons |
title_fullStr | Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons |
title_full_unstemmed | Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons |
title_short | Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons |
title_sort | peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8820717/ https://www.ncbi.nlm.nih.gov/pubmed/35017446 http://dx.doi.org/10.4103/1673-5374.332159 |
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