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Biofilm disruption and bactericidal activity of aqueous ozone coupled with ultrasonic dental scaling

BACKGROUND: The COVID-19 pandemic has heightened the awareness of a common hazard encountered in the dental clinic: aerosol transmission of pathogens. Treatment of sources of infection before or during dental procedures is one means of decreasing pathogen load and aerosol transmission. METHODS: An u...

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Autores principales: Failor, Kevin C., Silver, Bruce, Yu, Westin, Heindl, Jason E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Inc. on behalf of the American Dental Association. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8820975/
https://www.ncbi.nlm.nih.gov/pubmed/37520742
http://dx.doi.org/10.1016/j.jfscie.2021.100003
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author Failor, Kevin C.
Silver, Bruce
Yu, Westin
Heindl, Jason E.
author_facet Failor, Kevin C.
Silver, Bruce
Yu, Westin
Heindl, Jason E.
author_sort Failor, Kevin C.
collection PubMed
description BACKGROUND: The COVID-19 pandemic has heightened the awareness of a common hazard encountered in the dental clinic: aerosol transmission of pathogens. Treatment of sources of infection before or during dental procedures is one means of decreasing pathogen load and aerosol transmission. METHODS: An ultrasonic scaler supplied with aqueous ozone was used to examine the effect of its viability on planktonic cultures and biofilms formed by 2 model bacteria: Rothia mucilaginosa and Escherichia coli. RESULTS: Both organisms showed susceptibility to aqueous ozone alone (97% and 99.5% lethality, respectively). When combined with manual scaling using an ultrasonic scaler, a greater than 99% reduction in colony-forming units (CFUs)/mL could be reached with an aqueous ozone concentration of approximately 2 mg/L (R. mucilaginosa) or 0.75 mg/L (E. coli) after 5 through 6 seconds of scaling. CONCLUSIONS: Aqueous ozone coupled with ultrasonic scaling exhibited a higher efficiency of microbial kill than either method used alone. Both gram-positive and gram-negative species were affected by this treatment. Studies on other oral microbiota constituents, including fungi and viruses, will provide information on the efficacy of this method on a greater biological scale. Studies to verify concomitant reduction of microbial load in dispersed aerosols in clinical settings should be completed to support practical applications of this treatment.
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spelling pubmed-88209752022-02-08 Biofilm disruption and bactericidal activity of aqueous ozone coupled with ultrasonic dental scaling Failor, Kevin C. Silver, Bruce Yu, Westin Heindl, Jason E. JADA Foundational Science Research Article BACKGROUND: The COVID-19 pandemic has heightened the awareness of a common hazard encountered in the dental clinic: aerosol transmission of pathogens. Treatment of sources of infection before or during dental procedures is one means of decreasing pathogen load and aerosol transmission. METHODS: An ultrasonic scaler supplied with aqueous ozone was used to examine the effect of its viability on planktonic cultures and biofilms formed by 2 model bacteria: Rothia mucilaginosa and Escherichia coli. RESULTS: Both organisms showed susceptibility to aqueous ozone alone (97% and 99.5% lethality, respectively). When combined with manual scaling using an ultrasonic scaler, a greater than 99% reduction in colony-forming units (CFUs)/mL could be reached with an aqueous ozone concentration of approximately 2 mg/L (R. mucilaginosa) or 0.75 mg/L (E. coli) after 5 through 6 seconds of scaling. CONCLUSIONS: Aqueous ozone coupled with ultrasonic scaling exhibited a higher efficiency of microbial kill than either method used alone. Both gram-positive and gram-negative species were affected by this treatment. Studies on other oral microbiota constituents, including fungi and viruses, will provide information on the efficacy of this method on a greater biological scale. Studies to verify concomitant reduction of microbial load in dispersed aerosols in clinical settings should be completed to support practical applications of this treatment. Published by Elsevier Inc. on behalf of the American Dental Association. 2022 2022-02-08 /pmc/articles/PMC8820975/ /pubmed/37520742 http://dx.doi.org/10.1016/j.jfscie.2021.100003 Text en © 2021 The Author(s) Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Research Article
Failor, Kevin C.
Silver, Bruce
Yu, Westin
Heindl, Jason E.
Biofilm disruption and bactericidal activity of aqueous ozone coupled with ultrasonic dental scaling
title Biofilm disruption and bactericidal activity of aqueous ozone coupled with ultrasonic dental scaling
title_full Biofilm disruption and bactericidal activity of aqueous ozone coupled with ultrasonic dental scaling
title_fullStr Biofilm disruption and bactericidal activity of aqueous ozone coupled with ultrasonic dental scaling
title_full_unstemmed Biofilm disruption and bactericidal activity of aqueous ozone coupled with ultrasonic dental scaling
title_short Biofilm disruption and bactericidal activity of aqueous ozone coupled with ultrasonic dental scaling
title_sort biofilm disruption and bactericidal activity of aqueous ozone coupled with ultrasonic dental scaling
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8820975/
https://www.ncbi.nlm.nih.gov/pubmed/37520742
http://dx.doi.org/10.1016/j.jfscie.2021.100003
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