An archaellum filament composed of two alternating subunits

Archaea use a molecular machine, called the archaellum, to swim. The archaellum consists of an ATP-powered intracellular motor that drives the rotation of an extracellular filament composed of multiple copies of proteins named archaellins. In many species, several archaellin homologs are encoded in...

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Autores principales: Gambelli, Lavinia, Isupov, Michail N., Conners, Rebecca, McLaren, Mathew, Bellack, Annett, Gold, Vicki, Rachel, Reinhard, Daum, Bertram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8821640/
https://www.ncbi.nlm.nih.gov/pubmed/35132062
http://dx.doi.org/10.1038/s41467-022-28337-1
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author Gambelli, Lavinia
Isupov, Michail N.
Conners, Rebecca
McLaren, Mathew
Bellack, Annett
Gold, Vicki
Rachel, Reinhard
Daum, Bertram
author_facet Gambelli, Lavinia
Isupov, Michail N.
Conners, Rebecca
McLaren, Mathew
Bellack, Annett
Gold, Vicki
Rachel, Reinhard
Daum, Bertram
author_sort Gambelli, Lavinia
collection PubMed
description Archaea use a molecular machine, called the archaellum, to swim. The archaellum consists of an ATP-powered intracellular motor that drives the rotation of an extracellular filament composed of multiple copies of proteins named archaellins. In many species, several archaellin homologs are encoded in the same operon; however, previous structural studies indicated that archaellum filaments mainly consist of only one protein species. Here, we use electron cryo-microscopy to elucidate the structure of the archaellum from Methanocaldococcus villosus at 3.08 Å resolution. The filament is composed of two alternating archaellins, suggesting that the architecture and assembly of archaella is more complex than previously thought. Moreover, we identify structural elements that may contribute to the filament’s flexibility.
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spelling pubmed-88216402022-02-18 An archaellum filament composed of two alternating subunits Gambelli, Lavinia Isupov, Michail N. Conners, Rebecca McLaren, Mathew Bellack, Annett Gold, Vicki Rachel, Reinhard Daum, Bertram Nat Commun Article Archaea use a molecular machine, called the archaellum, to swim. The archaellum consists of an ATP-powered intracellular motor that drives the rotation of an extracellular filament composed of multiple copies of proteins named archaellins. In many species, several archaellin homologs are encoded in the same operon; however, previous structural studies indicated that archaellum filaments mainly consist of only one protein species. Here, we use electron cryo-microscopy to elucidate the structure of the archaellum from Methanocaldococcus villosus at 3.08 Å resolution. The filament is composed of two alternating archaellins, suggesting that the architecture and assembly of archaella is more complex than previously thought. Moreover, we identify structural elements that may contribute to the filament’s flexibility. Nature Publishing Group UK 2022-02-07 /pmc/articles/PMC8821640/ /pubmed/35132062 http://dx.doi.org/10.1038/s41467-022-28337-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Gambelli, Lavinia
Isupov, Michail N.
Conners, Rebecca
McLaren, Mathew
Bellack, Annett
Gold, Vicki
Rachel, Reinhard
Daum, Bertram
An archaellum filament composed of two alternating subunits
title An archaellum filament composed of two alternating subunits
title_full An archaellum filament composed of two alternating subunits
title_fullStr An archaellum filament composed of two alternating subunits
title_full_unstemmed An archaellum filament composed of two alternating subunits
title_short An archaellum filament composed of two alternating subunits
title_sort archaellum filament composed of two alternating subunits
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8821640/
https://www.ncbi.nlm.nih.gov/pubmed/35132062
http://dx.doi.org/10.1038/s41467-022-28337-1
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