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Introducing Nafion for In Situ Desalting and Biofluid Profiling in Spray Mass Spectrometry

We introduce Nafion into the ambient ionization technique of spray mass spectrometry to serve for in situ desalting and direct analysis of biological fluids. Nafion was coated onto the surface of the triangular spray tip as the cation exchange material. Because the sulfonic group from the Nafion mem...

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Autores principales: Song, Xiaowei, Mofidfar, Mohammad, Zare, Richard N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8821663/
https://www.ncbi.nlm.nih.gov/pubmed/35145954
http://dx.doi.org/10.3389/fchem.2021.807244
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author Song, Xiaowei
Mofidfar, Mohammad
Zare, Richard N.
author_facet Song, Xiaowei
Mofidfar, Mohammad
Zare, Richard N.
author_sort Song, Xiaowei
collection PubMed
description We introduce Nafion into the ambient ionization technique of spray mass spectrometry to serve for in situ desalting and direct analysis of biological fluids. Nafion was coated onto the surface of the triangular spray tip as the cation exchange material. Because the sulfonic group from the Nafion membrane effectively exchanges their carried protons with inorganic salt ions (e.g., Na(+) and K(+)), the analyte’s ionization efficiency can be significantly enhanced by reducing ion suppression. The desalting efficiency can reach 90% and the maximum tolerance of the absolute salt amount reaches 100 μmol. The mass spectral profile can also be simplified by removing the multiple adducted ion types from small-molecule drugs and metabolites ([M + Na](+) and [M + K](+)), or multiply charged ions formed by proteins ([M + nNa](n+) and [M + nK](n+)). Thus, the Nafion coating makes less ambiguous data interpretation collected from spray mass spectrometry for qualitative profiling or quantitative measurement of a target analyte.
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spelling pubmed-88216632022-02-09 Introducing Nafion for In Situ Desalting and Biofluid Profiling in Spray Mass Spectrometry Song, Xiaowei Mofidfar, Mohammad Zare, Richard N. Front Chem Chemistry We introduce Nafion into the ambient ionization technique of spray mass spectrometry to serve for in situ desalting and direct analysis of biological fluids. Nafion was coated onto the surface of the triangular spray tip as the cation exchange material. Because the sulfonic group from the Nafion membrane effectively exchanges their carried protons with inorganic salt ions (e.g., Na(+) and K(+)), the analyte’s ionization efficiency can be significantly enhanced by reducing ion suppression. The desalting efficiency can reach 90% and the maximum tolerance of the absolute salt amount reaches 100 μmol. The mass spectral profile can also be simplified by removing the multiple adducted ion types from small-molecule drugs and metabolites ([M + Na](+) and [M + K](+)), or multiply charged ions formed by proteins ([M + nNa](n+) and [M + nK](n+)). Thus, the Nafion coating makes less ambiguous data interpretation collected from spray mass spectrometry for qualitative profiling or quantitative measurement of a target analyte. Frontiers Media S.A. 2022-01-25 /pmc/articles/PMC8821663/ /pubmed/35145954 http://dx.doi.org/10.3389/fchem.2021.807244 Text en Copyright © 2022 Song, Mofidfar and Zare. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Song, Xiaowei
Mofidfar, Mohammad
Zare, Richard N.
Introducing Nafion for In Situ Desalting and Biofluid Profiling in Spray Mass Spectrometry
title Introducing Nafion for In Situ Desalting and Biofluid Profiling in Spray Mass Spectrometry
title_full Introducing Nafion for In Situ Desalting and Biofluid Profiling in Spray Mass Spectrometry
title_fullStr Introducing Nafion for In Situ Desalting and Biofluid Profiling in Spray Mass Spectrometry
title_full_unstemmed Introducing Nafion for In Situ Desalting and Biofluid Profiling in Spray Mass Spectrometry
title_short Introducing Nafion for In Situ Desalting and Biofluid Profiling in Spray Mass Spectrometry
title_sort introducing nafion for in situ desalting and biofluid profiling in spray mass spectrometry
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8821663/
https://www.ncbi.nlm.nih.gov/pubmed/35145954
http://dx.doi.org/10.3389/fchem.2021.807244
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