HR-Bac, a toolbox based on homologous recombination for expression, screening and production of multiprotein complexes using the baculovirus expression system

The Baculovirus/insect cell expression system is a powerful technology for reconstitution of eukaryotic macromolecular assemblies. Most multigene expression platforms rely on Tn7-mediated transposition for transferring the expression cassette into the baculoviral genome. This allows a rigorous chara...

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Detalles Bibliográficos
Autores principales: Kolesnikova, Olga, Zachayus, Amélie, Pichard, Simon, Osz, Judit, Rochel, Natacha, Rossolillo, Paola, Kolb-Cheynel, Isabelle, Troffer-Charlier, Nathalie, Compe, Emmanuel, Bensaude, Olivier, Berger, Imre, Poterszman, Arnaud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8821708/
https://www.ncbi.nlm.nih.gov/pubmed/35132103
http://dx.doi.org/10.1038/s41598-021-04715-5

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