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The Gut Microbiome and Metabolites Are Altered and Interrelated in Patients With Rheumatoid Arthritis
The relationship among the gut microbiome, global fecal metabolites and rheumatoid arthritis (RA) has not been systematically evaluated. In this study, we performed 16S rDNA sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based nontargeted metabolomic profiling on feces of 2...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8821809/ https://www.ncbi.nlm.nih.gov/pubmed/35145919 http://dx.doi.org/10.3389/fcimb.2021.763507 |
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author | Yu, Die Du, Juping Pu, Xia Zheng, Liyuan Chen, Shuaishuai Wang, Na Li, Jun Chen, Shiyong Pan, Shaobiao Shen, Bo |
author_facet | Yu, Die Du, Juping Pu, Xia Zheng, Liyuan Chen, Shuaishuai Wang, Na Li, Jun Chen, Shiyong Pan, Shaobiao Shen, Bo |
author_sort | Yu, Die |
collection | PubMed |
description | The relationship among the gut microbiome, global fecal metabolites and rheumatoid arthritis (RA) has not been systematically evaluated. In this study, we performed 16S rDNA sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based nontargeted metabolomic profiling on feces of 26 untreated RA patients and 26 healthy controls. Twenty-six genera and forty-one MS2-identified metabolites were significantly altered in the RA patients. Klebsiella, Escherichia, Eisenbergiella and Flavobacterium were more abundant in the RA patients, while Fusicatenibacter, Megamonas and Enterococcus were more abundant in the healthy controls. Function prediction analysis demonstrated that the biosynthesis pathways of amino acids, such as L-arginine and aromatic amino acids, were depleted in the RA group. In the metabolome results, fecal metabolites including glycerophospholipids (PC(18:3(9Z,12Z,15Z)/16:1(9Z)), lysoPE 19:1, lysoPE 18:0, lysoPC(18:0/0:0)), sphingolipids (Cer(d18:0/16:0), Cer(d18:0/12:0), Cer(d18:0/14:0)), kynurenic acid, xanthurenic acid and 3-hydroxyanthranilic acid were remarkably altered between the RA patients and healthy controls. Dysregulation of pathways, such as tryptophan metabolism, alpha-linolenic acid metabolism and glycerophospholipid metabolism, may contribute to the development of RA. Additionally, we revealed that the gut microbiome and metabolites were interrelated in the RA patients, while Escherichia was the core genus. By depicting the overall landscape of the intestinal microbiome and metabolome in RA patients, our study could provide possible novel research directions regarding RA pathogenesis and targeted therapy. |
format | Online Article Text |
id | pubmed-8821809 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88218092022-02-09 The Gut Microbiome and Metabolites Are Altered and Interrelated in Patients With Rheumatoid Arthritis Yu, Die Du, Juping Pu, Xia Zheng, Liyuan Chen, Shuaishuai Wang, Na Li, Jun Chen, Shiyong Pan, Shaobiao Shen, Bo Front Cell Infect Microbiol Cellular and Infection Microbiology The relationship among the gut microbiome, global fecal metabolites and rheumatoid arthritis (RA) has not been systematically evaluated. In this study, we performed 16S rDNA sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based nontargeted metabolomic profiling on feces of 26 untreated RA patients and 26 healthy controls. Twenty-six genera and forty-one MS2-identified metabolites were significantly altered in the RA patients. Klebsiella, Escherichia, Eisenbergiella and Flavobacterium were more abundant in the RA patients, while Fusicatenibacter, Megamonas and Enterococcus were more abundant in the healthy controls. Function prediction analysis demonstrated that the biosynthesis pathways of amino acids, such as L-arginine and aromatic amino acids, were depleted in the RA group. In the metabolome results, fecal metabolites including glycerophospholipids (PC(18:3(9Z,12Z,15Z)/16:1(9Z)), lysoPE 19:1, lysoPE 18:0, lysoPC(18:0/0:0)), sphingolipids (Cer(d18:0/16:0), Cer(d18:0/12:0), Cer(d18:0/14:0)), kynurenic acid, xanthurenic acid and 3-hydroxyanthranilic acid were remarkably altered between the RA patients and healthy controls. Dysregulation of pathways, such as tryptophan metabolism, alpha-linolenic acid metabolism and glycerophospholipid metabolism, may contribute to the development of RA. Additionally, we revealed that the gut microbiome and metabolites were interrelated in the RA patients, while Escherichia was the core genus. By depicting the overall landscape of the intestinal microbiome and metabolome in RA patients, our study could provide possible novel research directions regarding RA pathogenesis and targeted therapy. Frontiers Media S.A. 2022-01-25 /pmc/articles/PMC8821809/ /pubmed/35145919 http://dx.doi.org/10.3389/fcimb.2021.763507 Text en Copyright © 2022 Yu, Du, Pu, Zheng, Chen, Wang, Li, Chen, Pan and Shen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Yu, Die Du, Juping Pu, Xia Zheng, Liyuan Chen, Shuaishuai Wang, Na Li, Jun Chen, Shiyong Pan, Shaobiao Shen, Bo The Gut Microbiome and Metabolites Are Altered and Interrelated in Patients With Rheumatoid Arthritis |
title | The Gut Microbiome and Metabolites Are Altered and Interrelated in Patients With Rheumatoid Arthritis |
title_full | The Gut Microbiome and Metabolites Are Altered and Interrelated in Patients With Rheumatoid Arthritis |
title_fullStr | The Gut Microbiome and Metabolites Are Altered and Interrelated in Patients With Rheumatoid Arthritis |
title_full_unstemmed | The Gut Microbiome and Metabolites Are Altered and Interrelated in Patients With Rheumatoid Arthritis |
title_short | The Gut Microbiome and Metabolites Are Altered and Interrelated in Patients With Rheumatoid Arthritis |
title_sort | gut microbiome and metabolites are altered and interrelated in patients with rheumatoid arthritis |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8821809/ https://www.ncbi.nlm.nih.gov/pubmed/35145919 http://dx.doi.org/10.3389/fcimb.2021.763507 |
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