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Metabolic Engineering of Escherichia coli for Ectoine Production With a Fermentation Strategy of Supplementing the Amino Donor

Ectoine, an osmotic pressure-compensated solute, is used in the food, agriculture, medicine, and cosmetics industries due to its ability to protect macromolecules. In this study, an ectoine-producing variant of Escherichia coli, ET08, was genetically constructed by introducing the ectABC gene cluste...

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Autores principales: Zhang, Hao, Liang, Zhong, Zhao, Ming, Ma, Yanqin, Luo, Zhengshan, Li, Sha, Xu, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8822159/
https://www.ncbi.nlm.nih.gov/pubmed/35145959
http://dx.doi.org/10.3389/fbioe.2022.824859
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author Zhang, Hao
Liang, Zhong
Zhao, Ming
Ma, Yanqin
Luo, Zhengshan
Li, Sha
Xu, Hong
author_facet Zhang, Hao
Liang, Zhong
Zhao, Ming
Ma, Yanqin
Luo, Zhengshan
Li, Sha
Xu, Hong
author_sort Zhang, Hao
collection PubMed
description Ectoine, an osmotic pressure-compensated solute, is used in the food, agriculture, medicine, and cosmetics industries due to its ability to protect macromolecules. In this study, an ectoine-producing variant of Escherichia coli, ET08, was genetically constructed by introducing the ectABC gene cluster and eliminating metabolic pathways involving lysine and pyruvate. Medium optimization enhanced ectoine production from 1.87 to 10.2 g/L. Analysis of the transcriptional levels revealed that supplementation with ammonium sulfate enhanced the metabolic flux towards the biosynthesis of ectoine. Furthermore, by optimizing the copy number of ectA, ectB, and ectC, the recombinant E. coli ET11 (ectA:ectB:ectC = 1:2:1) produced 12.9 g/L ectoine in the shake flask and 53.2 g/L ectoine in a fed-batch fermenter, representing the highest ectoine titer produced by E. coli, which has great industrial prospects.
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spelling pubmed-88221592022-02-09 Metabolic Engineering of Escherichia coli for Ectoine Production With a Fermentation Strategy of Supplementing the Amino Donor Zhang, Hao Liang, Zhong Zhao, Ming Ma, Yanqin Luo, Zhengshan Li, Sha Xu, Hong Front Bioeng Biotechnol Bioengineering and Biotechnology Ectoine, an osmotic pressure-compensated solute, is used in the food, agriculture, medicine, and cosmetics industries due to its ability to protect macromolecules. In this study, an ectoine-producing variant of Escherichia coli, ET08, was genetically constructed by introducing the ectABC gene cluster and eliminating metabolic pathways involving lysine and pyruvate. Medium optimization enhanced ectoine production from 1.87 to 10.2 g/L. Analysis of the transcriptional levels revealed that supplementation with ammonium sulfate enhanced the metabolic flux towards the biosynthesis of ectoine. Furthermore, by optimizing the copy number of ectA, ectB, and ectC, the recombinant E. coli ET11 (ectA:ectB:ectC = 1:2:1) produced 12.9 g/L ectoine in the shake flask and 53.2 g/L ectoine in a fed-batch fermenter, representing the highest ectoine titer produced by E. coli, which has great industrial prospects. Frontiers Media S.A. 2022-01-25 /pmc/articles/PMC8822159/ /pubmed/35145959 http://dx.doi.org/10.3389/fbioe.2022.824859 Text en Copyright © 2022 Zhang, Liang, Zhao, Ma, Luo, Li and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Zhang, Hao
Liang, Zhong
Zhao, Ming
Ma, Yanqin
Luo, Zhengshan
Li, Sha
Xu, Hong
Metabolic Engineering of Escherichia coli for Ectoine Production With a Fermentation Strategy of Supplementing the Amino Donor
title Metabolic Engineering of Escherichia coli for Ectoine Production With a Fermentation Strategy of Supplementing the Amino Donor
title_full Metabolic Engineering of Escherichia coli for Ectoine Production With a Fermentation Strategy of Supplementing the Amino Donor
title_fullStr Metabolic Engineering of Escherichia coli for Ectoine Production With a Fermentation Strategy of Supplementing the Amino Donor
title_full_unstemmed Metabolic Engineering of Escherichia coli for Ectoine Production With a Fermentation Strategy of Supplementing the Amino Donor
title_short Metabolic Engineering of Escherichia coli for Ectoine Production With a Fermentation Strategy of Supplementing the Amino Donor
title_sort metabolic engineering of escherichia coli for ectoine production with a fermentation strategy of supplementing the amino donor
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8822159/
https://www.ncbi.nlm.nih.gov/pubmed/35145959
http://dx.doi.org/10.3389/fbioe.2022.824859
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