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Inhibitory effects of superoxide dismutase 3 on IgE production in B cells

Immunoglobulin E (IgE) functions as a first-line defense against parasitic infections. However, aberrant production of IgE is known to be associated with various life-threatening allergic diseases. Superoxide dismutase 3 (SOD3) has been found to suppress IgE in various allergic diseases such as alle...

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Autores principales: Agrahari, Gaurav, Sah, Shyam Kishor, Lee, Min Jung, Bang, Chul Hwan, Kim, Yeong Ho, Kim, Hey-Young, Kim, Tae-Yoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8822298/
https://www.ncbi.nlm.nih.gov/pubmed/35155837
http://dx.doi.org/10.1016/j.bbrep.2022.101226
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author Agrahari, Gaurav
Sah, Shyam Kishor
Lee, Min Jung
Bang, Chul Hwan
Kim, Yeong Ho
Kim, Hey-Young
Kim, Tae-Yoon
author_facet Agrahari, Gaurav
Sah, Shyam Kishor
Lee, Min Jung
Bang, Chul Hwan
Kim, Yeong Ho
Kim, Hey-Young
Kim, Tae-Yoon
author_sort Agrahari, Gaurav
collection PubMed
description Immunoglobulin E (IgE) functions as a first-line defense against parasitic infections. However, aberrant production of IgE is known to be associated with various life-threatening allergic diseases. Superoxide dismutase 3 (SOD3) has been found to suppress IgE in various allergic diseases such as allergic conjunctivitis, ovalbumin-induced allergic asthma, and dust mite-induced atopic dermatitis-like skin inflammation. However, the role of SOD3 in the regulation of IgE production in B cells remains elusive. In this study, we investigated the effect of SOD3 on LPS/IL-4 and anti-CD40/IL-4-mediated secretion of IgE in murine B cells. Our data showed that SOD3 can suppress both LPS/IL-4 and antiCD40/IL-7-induced IgE secretion in B cells isolated from both wild-type (SOD3(+/+)) and SOD3 knock-out (SOD3(−/−)) mice. Interestingly, B cells isolated from SOD3(−/−) mice showed higher secretion of IgE, whereas, the use of DETCA, a known inhibitor of SOD3 activity, reversed the inhibitory effect of SOD3 on IgE production. Similarly, SOD3 was found to reduce the proliferation, IgE isotype switch, ROS level, and CCL17 and CCL22 productions in B cells. Furthermore, SOD3 was found to suppress both LPS/IL-4 and anti-CD40/IL-4-mediated activation of downstream signaling such as JAK1/JAK3, STAT6, NF-κB, p38, and JNK in B cells. Taken together, our data showed that SOD3 can be used as an alternative therapy to restrict IgE-mediated allergic diseases.
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spelling pubmed-88222982022-02-11 Inhibitory effects of superoxide dismutase 3 on IgE production in B cells Agrahari, Gaurav Sah, Shyam Kishor Lee, Min Jung Bang, Chul Hwan Kim, Yeong Ho Kim, Hey-Young Kim, Tae-Yoon Biochem Biophys Rep Research Article Immunoglobulin E (IgE) functions as a first-line defense against parasitic infections. However, aberrant production of IgE is known to be associated with various life-threatening allergic diseases. Superoxide dismutase 3 (SOD3) has been found to suppress IgE in various allergic diseases such as allergic conjunctivitis, ovalbumin-induced allergic asthma, and dust mite-induced atopic dermatitis-like skin inflammation. However, the role of SOD3 in the regulation of IgE production in B cells remains elusive. In this study, we investigated the effect of SOD3 on LPS/IL-4 and anti-CD40/IL-4-mediated secretion of IgE in murine B cells. Our data showed that SOD3 can suppress both LPS/IL-4 and antiCD40/IL-7-induced IgE secretion in B cells isolated from both wild-type (SOD3(+/+)) and SOD3 knock-out (SOD3(−/−)) mice. Interestingly, B cells isolated from SOD3(−/−) mice showed higher secretion of IgE, whereas, the use of DETCA, a known inhibitor of SOD3 activity, reversed the inhibitory effect of SOD3 on IgE production. Similarly, SOD3 was found to reduce the proliferation, IgE isotype switch, ROS level, and CCL17 and CCL22 productions in B cells. Furthermore, SOD3 was found to suppress both LPS/IL-4 and anti-CD40/IL-4-mediated activation of downstream signaling such as JAK1/JAK3, STAT6, NF-κB, p38, and JNK in B cells. Taken together, our data showed that SOD3 can be used as an alternative therapy to restrict IgE-mediated allergic diseases. Elsevier 2022-02-03 /pmc/articles/PMC8822298/ /pubmed/35155837 http://dx.doi.org/10.1016/j.bbrep.2022.101226 Text en © 2022 The Authors. Published by Elsevier B.V. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Agrahari, Gaurav
Sah, Shyam Kishor
Lee, Min Jung
Bang, Chul Hwan
Kim, Yeong Ho
Kim, Hey-Young
Kim, Tae-Yoon
Inhibitory effects of superoxide dismutase 3 on IgE production in B cells
title Inhibitory effects of superoxide dismutase 3 on IgE production in B cells
title_full Inhibitory effects of superoxide dismutase 3 on IgE production in B cells
title_fullStr Inhibitory effects of superoxide dismutase 3 on IgE production in B cells
title_full_unstemmed Inhibitory effects of superoxide dismutase 3 on IgE production in B cells
title_short Inhibitory effects of superoxide dismutase 3 on IgE production in B cells
title_sort inhibitory effects of superoxide dismutase 3 on ige production in b cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8822298/
https://www.ncbi.nlm.nih.gov/pubmed/35155837
http://dx.doi.org/10.1016/j.bbrep.2022.101226
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