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4362 The Utilization of Polyethylene Glycol Fusion to Improve Facial Reanimation

OBJECTIVES/GOALS: This study’s goal is to determine whether intraoperative treatment of facial nerves with polyethylene glycol (PEG) fusion technology improves facial paralysis outcomes. Improved facial nerve regeneration in facial paralysis patients would lead to improved recovery time and effectiv...

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Autores principales: Suchyta, Marissa, Roh, Si-Gyun, Sabbagh, Diya, Morsy, Mohammed, Wang, Huan, Mardini, Samir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cambridge University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8823309/
http://dx.doi.org/10.1017/cts.2020.319
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author Suchyta, Marissa
Roh, Si-Gyun
Sabbagh, Diya
Morsy, Mohammed
Wang, Huan
Mardini, Samir
author_facet Suchyta, Marissa
Roh, Si-Gyun
Sabbagh, Diya
Morsy, Mohammed
Wang, Huan
Mardini, Samir
author_sort Suchyta, Marissa
collection PubMed
description OBJECTIVES/GOALS: This study’s goal is to determine whether intraoperative treatment of facial nerves with polyethylene glycol (PEG) fusion technology improves facial paralysis outcomes. Improved facial nerve regeneration in facial paralysis patients would lead to improved recovery time and effectiveness. METHODS/STUDY POPULATION: 30 rats were utilized; 15 underwent facial nerve regeneration without PEG fusion, and 15 with PEG fusion. Facial paralysis was initiated on the left by transection of the buccal and marginal mandibular branches of facial nerve. The buccal branch was repaired though microsuture technique. Neurorrhaphy sites of rats in the PEG group were exposed to calcium free saline, methylene blue, and polyethylene glycol. Nerve continuity was assessed post-operative in 5 animals in each group through electron microscopy. Functionality was assessed in the other 10 per group by EMG and whisker analysis after surgery, and weekly for 8 weeks. At 8 weeks, nerves and distal muscles were histologically analyzed. RESULTS/ANTICIPATED RESULTS: PEG fusion technology immediately restored axonal continuity following surgery, demonstrated by electron microscopy. Electrophysiology was also similarly restored across the site immediately, determined through intraoperative nerve stimulation, in the PEG fusion group. The nonintervention group showed dramatically reduced functional recovery than the PEG fusion group following surgery, shown by lower whisking activity and poor electrophysiology outcomes. Furthermore, the PEG fusion group showed statistically significant higher fascicle counts, myelination diameter, axonal diameter, and distal muscle fibers histologically. DISCUSSION/SIGNIFICANCE OF IMPACT: This study demonstrates that polyethylene fusion technology may improve facial reanimation outcomes. PEG is already a FDA-approved drug, and thus the pathway to translational clinical application of this work may thus be streamlined, bringing new options to patients with facial paralysis.
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spelling pubmed-88233092022-02-18 4362 The Utilization of Polyethylene Glycol Fusion to Improve Facial Reanimation Suchyta, Marissa Roh, Si-Gyun Sabbagh, Diya Morsy, Mohammed Wang, Huan Mardini, Samir J Clin Transl Sci Mechanistic Basic to Clinical OBJECTIVES/GOALS: This study’s goal is to determine whether intraoperative treatment of facial nerves with polyethylene glycol (PEG) fusion technology improves facial paralysis outcomes. Improved facial nerve regeneration in facial paralysis patients would lead to improved recovery time and effectiveness. METHODS/STUDY POPULATION: 30 rats were utilized; 15 underwent facial nerve regeneration without PEG fusion, and 15 with PEG fusion. Facial paralysis was initiated on the left by transection of the buccal and marginal mandibular branches of facial nerve. The buccal branch was repaired though microsuture technique. Neurorrhaphy sites of rats in the PEG group were exposed to calcium free saline, methylene blue, and polyethylene glycol. Nerve continuity was assessed post-operative in 5 animals in each group through electron microscopy. Functionality was assessed in the other 10 per group by EMG and whisker analysis after surgery, and weekly for 8 weeks. At 8 weeks, nerves and distal muscles were histologically analyzed. RESULTS/ANTICIPATED RESULTS: PEG fusion technology immediately restored axonal continuity following surgery, demonstrated by electron microscopy. Electrophysiology was also similarly restored across the site immediately, determined through intraoperative nerve stimulation, in the PEG fusion group. The nonintervention group showed dramatically reduced functional recovery than the PEG fusion group following surgery, shown by lower whisking activity and poor electrophysiology outcomes. Furthermore, the PEG fusion group showed statistically significant higher fascicle counts, myelination diameter, axonal diameter, and distal muscle fibers histologically. DISCUSSION/SIGNIFICANCE OF IMPACT: This study demonstrates that polyethylene fusion technology may improve facial reanimation outcomes. PEG is already a FDA-approved drug, and thus the pathway to translational clinical application of this work may thus be streamlined, bringing new options to patients with facial paralysis. Cambridge University Press 2020-07-29 /pmc/articles/PMC8823309/ http://dx.doi.org/10.1017/cts.2020.319 Text en © The Association for Clinical and Translational Science 2020 https://creativecommons.org/licenses/by/4.0/This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Mechanistic Basic to Clinical
Suchyta, Marissa
Roh, Si-Gyun
Sabbagh, Diya
Morsy, Mohammed
Wang, Huan
Mardini, Samir
4362 The Utilization of Polyethylene Glycol Fusion to Improve Facial Reanimation
title 4362 The Utilization of Polyethylene Glycol Fusion to Improve Facial Reanimation
title_full 4362 The Utilization of Polyethylene Glycol Fusion to Improve Facial Reanimation
title_fullStr 4362 The Utilization of Polyethylene Glycol Fusion to Improve Facial Reanimation
title_full_unstemmed 4362 The Utilization of Polyethylene Glycol Fusion to Improve Facial Reanimation
title_short 4362 The Utilization of Polyethylene Glycol Fusion to Improve Facial Reanimation
title_sort 4362 the utilization of polyethylene glycol fusion to improve facial reanimation
topic Mechanistic Basic to Clinical
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8823309/
http://dx.doi.org/10.1017/cts.2020.319
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