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Heterochiasmy and the establishment of gsdf as a novel sex determining gene in Atlantic halibut

Atlantic Halibut (Hippoglossus hippoglossus) has a X/Y genetic sex determination system, but the sex determining factor is not known. We produced a high-quality genome assembly from a male and identified parts of chromosome 13 as the Y chromosome due to sequence divergence between sexes and segregat...

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Autores principales: Edvardsen, Rolf Brudvik, Wallerman, Ola, Furmanek, Tomasz, Kleppe, Lene, Jern, Patric, Wallberg, Andreas, Kjærner-Semb, Erik, Mæhle, Stig, Olausson, Sara Karolina, Sundström, Elisabeth, Harboe, Torstein, Mangor-Jensen, Ragnfrid, Møgster, Margareth, Perrichon, Prescilla, Norberg, Birgitta, Rubin, Carl-Johan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8824383/
https://www.ncbi.nlm.nih.gov/pubmed/35134055
http://dx.doi.org/10.1371/journal.pgen.1010011
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author Edvardsen, Rolf Brudvik
Wallerman, Ola
Furmanek, Tomasz
Kleppe, Lene
Jern, Patric
Wallberg, Andreas
Kjærner-Semb, Erik
Mæhle, Stig
Olausson, Sara Karolina
Sundström, Elisabeth
Harboe, Torstein
Mangor-Jensen, Ragnfrid
Møgster, Margareth
Perrichon, Prescilla
Norberg, Birgitta
Rubin, Carl-Johan
author_facet Edvardsen, Rolf Brudvik
Wallerman, Ola
Furmanek, Tomasz
Kleppe, Lene
Jern, Patric
Wallberg, Andreas
Kjærner-Semb, Erik
Mæhle, Stig
Olausson, Sara Karolina
Sundström, Elisabeth
Harboe, Torstein
Mangor-Jensen, Ragnfrid
Møgster, Margareth
Perrichon, Prescilla
Norberg, Birgitta
Rubin, Carl-Johan
author_sort Edvardsen, Rolf Brudvik
collection PubMed
description Atlantic Halibut (Hippoglossus hippoglossus) has a X/Y genetic sex determination system, but the sex determining factor is not known. We produced a high-quality genome assembly from a male and identified parts of chromosome 13 as the Y chromosome due to sequence divergence between sexes and segregation of sex genotypes in pedigrees. Linkage analysis revealed that all chromosomes exhibit heterochiasmy, i.e. male-only and female-only meiotic recombination regions (MRR/FRR). We show that FRR/MRR intervals differ in nucleotide diversity and repeat class content and that this is true also for other Pleuronectidae species. We further show that remnants of a Gypsy-like transposable element insertion on chr13 promotes early male specific expression of gonadal somatic cell derived factor (gsdf). Less than 4.5 MYA, this male-determining element evolved on an autosomal FRR segment featuring pre-existing male meiotic recombination barriers, thereby creating a Y chromosome. Our findings indicate that heterochiasmy may facilitate the evolution of genetic sex determination systems relying on linkage of sexually antagonistic loci to a sex-determining factor.
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spelling pubmed-88243832022-02-09 Heterochiasmy and the establishment of gsdf as a novel sex determining gene in Atlantic halibut Edvardsen, Rolf Brudvik Wallerman, Ola Furmanek, Tomasz Kleppe, Lene Jern, Patric Wallberg, Andreas Kjærner-Semb, Erik Mæhle, Stig Olausson, Sara Karolina Sundström, Elisabeth Harboe, Torstein Mangor-Jensen, Ragnfrid Møgster, Margareth Perrichon, Prescilla Norberg, Birgitta Rubin, Carl-Johan PLoS Genet Research Article Atlantic Halibut (Hippoglossus hippoglossus) has a X/Y genetic sex determination system, but the sex determining factor is not known. We produced a high-quality genome assembly from a male and identified parts of chromosome 13 as the Y chromosome due to sequence divergence between sexes and segregation of sex genotypes in pedigrees. Linkage analysis revealed that all chromosomes exhibit heterochiasmy, i.e. male-only and female-only meiotic recombination regions (MRR/FRR). We show that FRR/MRR intervals differ in nucleotide diversity and repeat class content and that this is true also for other Pleuronectidae species. We further show that remnants of a Gypsy-like transposable element insertion on chr13 promotes early male specific expression of gonadal somatic cell derived factor (gsdf). Less than 4.5 MYA, this male-determining element evolved on an autosomal FRR segment featuring pre-existing male meiotic recombination barriers, thereby creating a Y chromosome. Our findings indicate that heterochiasmy may facilitate the evolution of genetic sex determination systems relying on linkage of sexually antagonistic loci to a sex-determining factor. Public Library of Science 2022-02-08 /pmc/articles/PMC8824383/ /pubmed/35134055 http://dx.doi.org/10.1371/journal.pgen.1010011 Text en © 2022 Edvardsen et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Edvardsen, Rolf Brudvik
Wallerman, Ola
Furmanek, Tomasz
Kleppe, Lene
Jern, Patric
Wallberg, Andreas
Kjærner-Semb, Erik
Mæhle, Stig
Olausson, Sara Karolina
Sundström, Elisabeth
Harboe, Torstein
Mangor-Jensen, Ragnfrid
Møgster, Margareth
Perrichon, Prescilla
Norberg, Birgitta
Rubin, Carl-Johan
Heterochiasmy and the establishment of gsdf as a novel sex determining gene in Atlantic halibut
title Heterochiasmy and the establishment of gsdf as a novel sex determining gene in Atlantic halibut
title_full Heterochiasmy and the establishment of gsdf as a novel sex determining gene in Atlantic halibut
title_fullStr Heterochiasmy and the establishment of gsdf as a novel sex determining gene in Atlantic halibut
title_full_unstemmed Heterochiasmy and the establishment of gsdf as a novel sex determining gene in Atlantic halibut
title_short Heterochiasmy and the establishment of gsdf as a novel sex determining gene in Atlantic halibut
title_sort heterochiasmy and the establishment of gsdf as a novel sex determining gene in atlantic halibut
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8824383/
https://www.ncbi.nlm.nih.gov/pubmed/35134055
http://dx.doi.org/10.1371/journal.pgen.1010011
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