The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis?

Several commercial assays for SARS-CoV-2 RT-PCR are available but few of them were assessed. We evaluate the Allplex 2019-nCoV (Seegene) assay using 41 nasopharyngeal samples. The rates of agreement were 92.7% and 100% with the GeneFinder COVID-19 plus (Elitech) and the diagnosis of the infectious d...

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Autores principales: Farfour, Eric, Lesprit, Philippe, Visseaux, Benoit, Pascreau, Tiffany, Jolly, Emilie, Houhou, Nadira, Mazaux, Laurence, Asso-Bonnet, Marianne, Vasse, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8824685/
https://www.ncbi.nlm.nih.gov/pubmed/32462501
http://dx.doi.org/10.1007/s10096-020-03930-8
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author Farfour, Eric
Lesprit, Philippe
Visseaux, Benoit
Pascreau, Tiffany
Jolly, Emilie
Houhou, Nadira
Mazaux, Laurence
Asso-Bonnet, Marianne
Vasse, Marc
author_facet Farfour, Eric
Lesprit, Philippe
Visseaux, Benoit
Pascreau, Tiffany
Jolly, Emilie
Houhou, Nadira
Mazaux, Laurence
Asso-Bonnet, Marianne
Vasse, Marc
author_sort Farfour, Eric
collection PubMed
description Several commercial assays for SARS-CoV-2 RT-PCR are available but few of them were assessed. We evaluate the Allplex 2019-nCoV (Seegene) assay using 41 nasopharyngeal samples. The rates of agreement were 92.7% and 100% with the GeneFinder COVID-19 plus (Elitech) and the diagnosis of the infectious disease specialist respectively. Four samples display a Ct < 22.0 for the E and RdRp genes while the N gene was not detected, suggesting a variability of the viral sequence. There was no cross-reactivity with other respiratory viruses. The Allplex 2019-nCoV appears as a reliable method, but additional evaluations using more samples are needed. RT-PCR assays should probably include at least 2 viral targets.
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spelling pubmed-88246852022-02-09 The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis? Farfour, Eric Lesprit, Philippe Visseaux, Benoit Pascreau, Tiffany Jolly, Emilie Houhou, Nadira Mazaux, Laurence Asso-Bonnet, Marianne Vasse, Marc Eur J Clin Microbiol Infect Dis Brief Report Several commercial assays for SARS-CoV-2 RT-PCR are available but few of them were assessed. We evaluate the Allplex 2019-nCoV (Seegene) assay using 41 nasopharyngeal samples. The rates of agreement were 92.7% and 100% with the GeneFinder COVID-19 plus (Elitech) and the diagnosis of the infectious disease specialist respectively. Four samples display a Ct < 22.0 for the E and RdRp genes while the N gene was not detected, suggesting a variability of the viral sequence. There was no cross-reactivity with other respiratory viruses. The Allplex 2019-nCoV appears as a reliable method, but additional evaluations using more samples are needed. RT-PCR assays should probably include at least 2 viral targets. Springer Berlin Heidelberg 2020-05-28 2020 /pmc/articles/PMC8824685/ /pubmed/32462501 http://dx.doi.org/10.1007/s10096-020-03930-8 Text en © Springer-Verlag GmbH Germany, part of Springer Nature 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Farfour, Eric
Lesprit, Philippe
Visseaux, Benoit
Pascreau, Tiffany
Jolly, Emilie
Houhou, Nadira
Mazaux, Laurence
Asso-Bonnet, Marianne
Vasse, Marc
The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis?
title The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis?
title_full The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis?
title_fullStr The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis?
title_full_unstemmed The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis?
title_short The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis?
title_sort allplex 2019-ncov (seegene) assay: which performances are for sars-cov-2 infection diagnosis?
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8824685/
https://www.ncbi.nlm.nih.gov/pubmed/32462501
http://dx.doi.org/10.1007/s10096-020-03930-8
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