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Rutaecarpine suppresses the proliferation and metastasis of colon cancer cells by regulating the STAT3 signaling

Colorectal cancer (CRC) is a malignant disease that is a serious threat to human health. Rutaecarpine (RUT) is an important bioactive alkaloid of Evodia rutaecarpa. According to previous studies, RUT suppressed the proliferation of several human tumors. However, its role in colorectal tumorigenesis...

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Autores principales: Chan, Shixin, Sun, Rui, Tu, Xucan, Guo, Manyu, Yuan, Qianqian, Yu, Zhen, Wang, Zhenglin, Hong, Shaocheng, Han, Wei, Zou, Bingbing, Li, Zeng, Zhang, Huabing, Chen, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8824880/
https://www.ncbi.nlm.nih.gov/pubmed/35154453
http://dx.doi.org/10.7150/jca.66177
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author Chan, Shixin
Sun, Rui
Tu, Xucan
Guo, Manyu
Yuan, Qianqian
Yu, Zhen
Wang, Zhenglin
Hong, Shaocheng
Han, Wei
Zou, Bingbing
Li, Zeng
Zhang, Huabing
Chen, Wei
author_facet Chan, Shixin
Sun, Rui
Tu, Xucan
Guo, Manyu
Yuan, Qianqian
Yu, Zhen
Wang, Zhenglin
Hong, Shaocheng
Han, Wei
Zou, Bingbing
Li, Zeng
Zhang, Huabing
Chen, Wei
author_sort Chan, Shixin
collection PubMed
description Colorectal cancer (CRC) is a malignant disease that is a serious threat to human health. Rutaecarpine (RUT) is an important bioactive alkaloid of Evodia rutaecarpa. According to previous studies, RUT suppressed the proliferation of several human tumors. However, its role in colorectal tumorigenesis remained unknown. The aim of the present study was to determine the functions of RUT in CRC. Here, we have demonstrated that RUT inhibited the proliferation, migration and invasion of CRC cells in vitro. Further, RUT was found to induce the apoptosis of CRC cells. Mechanistically, RUT decreased the phosphorylation levels of NF-κB and STAT3. Moreover, treatment with RUT upregulated the expression of cleaved-Caspase3 and downregulated the expression of Bcl-2 in CRC. In addition, our findings suggested that RUT inhibited the growth and lung metastasis of CRC Cells in vivo. Based on immunofluorescence analysis, the expression of Ki67 was downregulated while that of cleaved-Caspase3 was upregulated in RUT-treated tumors compared with control-treated tumors. Taken together, our findings indicate that RUT can inhibit the proliferation and migration of CRC cells, and induce the apoptosis of CRC cells by inactivating NF-κB/STAT3 signaling. Our study highlights the potential clinical application of RUT for the treatment of CRC.
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spelling pubmed-88248802022-02-11 Rutaecarpine suppresses the proliferation and metastasis of colon cancer cells by regulating the STAT3 signaling Chan, Shixin Sun, Rui Tu, Xucan Guo, Manyu Yuan, Qianqian Yu, Zhen Wang, Zhenglin Hong, Shaocheng Han, Wei Zou, Bingbing Li, Zeng Zhang, Huabing Chen, Wei J Cancer Research Paper Colorectal cancer (CRC) is a malignant disease that is a serious threat to human health. Rutaecarpine (RUT) is an important bioactive alkaloid of Evodia rutaecarpa. According to previous studies, RUT suppressed the proliferation of several human tumors. However, its role in colorectal tumorigenesis remained unknown. The aim of the present study was to determine the functions of RUT in CRC. Here, we have demonstrated that RUT inhibited the proliferation, migration and invasion of CRC cells in vitro. Further, RUT was found to induce the apoptosis of CRC cells. Mechanistically, RUT decreased the phosphorylation levels of NF-κB and STAT3. Moreover, treatment with RUT upregulated the expression of cleaved-Caspase3 and downregulated the expression of Bcl-2 in CRC. In addition, our findings suggested that RUT inhibited the growth and lung metastasis of CRC Cells in vivo. Based on immunofluorescence analysis, the expression of Ki67 was downregulated while that of cleaved-Caspase3 was upregulated in RUT-treated tumors compared with control-treated tumors. Taken together, our findings indicate that RUT can inhibit the proliferation and migration of CRC cells, and induce the apoptosis of CRC cells by inactivating NF-κB/STAT3 signaling. Our study highlights the potential clinical application of RUT for the treatment of CRC. Ivyspring International Publisher 2022-01-01 /pmc/articles/PMC8824880/ /pubmed/35154453 http://dx.doi.org/10.7150/jca.66177 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Chan, Shixin
Sun, Rui
Tu, Xucan
Guo, Manyu
Yuan, Qianqian
Yu, Zhen
Wang, Zhenglin
Hong, Shaocheng
Han, Wei
Zou, Bingbing
Li, Zeng
Zhang, Huabing
Chen, Wei
Rutaecarpine suppresses the proliferation and metastasis of colon cancer cells by regulating the STAT3 signaling
title Rutaecarpine suppresses the proliferation and metastasis of colon cancer cells by regulating the STAT3 signaling
title_full Rutaecarpine suppresses the proliferation and metastasis of colon cancer cells by regulating the STAT3 signaling
title_fullStr Rutaecarpine suppresses the proliferation and metastasis of colon cancer cells by regulating the STAT3 signaling
title_full_unstemmed Rutaecarpine suppresses the proliferation and metastasis of colon cancer cells by regulating the STAT3 signaling
title_short Rutaecarpine suppresses the proliferation and metastasis of colon cancer cells by regulating the STAT3 signaling
title_sort rutaecarpine suppresses the proliferation and metastasis of colon cancer cells by regulating the stat3 signaling
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8824880/
https://www.ncbi.nlm.nih.gov/pubmed/35154453
http://dx.doi.org/10.7150/jca.66177
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