Cargando…
Monitoring Distribution Dynamics of EV RNA Cargo Within Recipient Monocytes and Macrophages
Extracellular vesicles (EVs) are produced by across almost all the living kingdoms and play a crucial role in cell-cell communication processes. EVs are especially important for pathogens, as Plasmodium falciparum (Pf) parasite, the leading causing species in human malaria. Malaria parasites are abl...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8825493/ https://www.ncbi.nlm.nih.gov/pubmed/35155269 http://dx.doi.org/10.3389/fcimb.2021.739628 |
_version_ | 1784647221123219456 |
---|---|
author | Alfandari, Daniel Ben Ami Pilo, Hila Abou Karam, Paula Dagan, Osnat Joubran, Carine Rotkopf, Ron Regev-Rudzki, Neta Porat, Ziv |
author_facet | Alfandari, Daniel Ben Ami Pilo, Hila Abou Karam, Paula Dagan, Osnat Joubran, Carine Rotkopf, Ron Regev-Rudzki, Neta Porat, Ziv |
author_sort | Alfandari, Daniel |
collection | PubMed |
description | Extracellular vesicles (EVs) are produced by across almost all the living kingdoms and play a crucial role in cell-cell communication processes. EVs are especially important for pathogens, as Plasmodium falciparum (Pf) parasite, the leading causing species in human malaria. Malaria parasites are able to modulate the host immune response from a distance via delivering diverse cargo components inside the EVs, such as proteins and nucleic acids. We have previously shown that imaging flow cytometry (IFC) can be effectively used to monitor the uptake of different cargo components of malaria derived EVs by host human monocytes. Here, we take this approach one step further and demonstrate that we can directly investigate the dynamics of the cargo distribution pattern over time by monitoring its distribution within two different recipient cells of the immune system, monocytes vs macrophages. By staining the RNA cargo of the vesicles and monitor the signal we were able to evaluate the kinetics of its delivery and measure different parameters of the cargo’s distribution post internalization. Interestingly, we found that while the level of the EV uptake is similar, the pattern of the signal for RNA cargo distribution is significantly different between these two recipient immune cells. Our results demonstrate that this method can be applied to study the distribution dynamics of the vesicle cargo post uptake to different types of cells. This can benefit significantly to our understanding of the fate of cargo components post vesicle internalization in the complex interface between pathogen-derived vesicles and their host recipient cells. |
format | Online Article Text |
id | pubmed-8825493 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88254932022-02-10 Monitoring Distribution Dynamics of EV RNA Cargo Within Recipient Monocytes and Macrophages Alfandari, Daniel Ben Ami Pilo, Hila Abou Karam, Paula Dagan, Osnat Joubran, Carine Rotkopf, Ron Regev-Rudzki, Neta Porat, Ziv Front Cell Infect Microbiol Cellular and Infection Microbiology Extracellular vesicles (EVs) are produced by across almost all the living kingdoms and play a crucial role in cell-cell communication processes. EVs are especially important for pathogens, as Plasmodium falciparum (Pf) parasite, the leading causing species in human malaria. Malaria parasites are able to modulate the host immune response from a distance via delivering diverse cargo components inside the EVs, such as proteins and nucleic acids. We have previously shown that imaging flow cytometry (IFC) can be effectively used to monitor the uptake of different cargo components of malaria derived EVs by host human monocytes. Here, we take this approach one step further and demonstrate that we can directly investigate the dynamics of the cargo distribution pattern over time by monitoring its distribution within two different recipient cells of the immune system, monocytes vs macrophages. By staining the RNA cargo of the vesicles and monitor the signal we were able to evaluate the kinetics of its delivery and measure different parameters of the cargo’s distribution post internalization. Interestingly, we found that while the level of the EV uptake is similar, the pattern of the signal for RNA cargo distribution is significantly different between these two recipient immune cells. Our results demonstrate that this method can be applied to study the distribution dynamics of the vesicle cargo post uptake to different types of cells. This can benefit significantly to our understanding of the fate of cargo components post vesicle internalization in the complex interface between pathogen-derived vesicles and their host recipient cells. Frontiers Media S.A. 2022-01-26 /pmc/articles/PMC8825493/ /pubmed/35155269 http://dx.doi.org/10.3389/fcimb.2021.739628 Text en Copyright © 2022 Alfandari, Ben Ami Pilo, Abou Karam, Dagan, Joubran, Rotkopf, Regev-Rudzki and Porat https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Alfandari, Daniel Ben Ami Pilo, Hila Abou Karam, Paula Dagan, Osnat Joubran, Carine Rotkopf, Ron Regev-Rudzki, Neta Porat, Ziv Monitoring Distribution Dynamics of EV RNA Cargo Within Recipient Monocytes and Macrophages |
title | Monitoring Distribution Dynamics of EV RNA Cargo Within Recipient Monocytes and Macrophages |
title_full | Monitoring Distribution Dynamics of EV RNA Cargo Within Recipient Monocytes and Macrophages |
title_fullStr | Monitoring Distribution Dynamics of EV RNA Cargo Within Recipient Monocytes and Macrophages |
title_full_unstemmed | Monitoring Distribution Dynamics of EV RNA Cargo Within Recipient Monocytes and Macrophages |
title_short | Monitoring Distribution Dynamics of EV RNA Cargo Within Recipient Monocytes and Macrophages |
title_sort | monitoring distribution dynamics of ev rna cargo within recipient monocytes and macrophages |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8825493/ https://www.ncbi.nlm.nih.gov/pubmed/35155269 http://dx.doi.org/10.3389/fcimb.2021.739628 |
work_keys_str_mv | AT alfandaridaniel monitoringdistributiondynamicsofevrnacargowithinrecipientmonocytesandmacrophages AT benamipilohila monitoringdistributiondynamicsofevrnacargowithinrecipientmonocytesandmacrophages AT aboukarampaula monitoringdistributiondynamicsofevrnacargowithinrecipientmonocytesandmacrophages AT daganosnat monitoringdistributiondynamicsofevrnacargowithinrecipientmonocytesandmacrophages AT joubrancarine monitoringdistributiondynamicsofevrnacargowithinrecipientmonocytesandmacrophages AT rotkopfron monitoringdistributiondynamicsofevrnacargowithinrecipientmonocytesandmacrophages AT regevrudzkineta monitoringdistributiondynamicsofevrnacargowithinrecipientmonocytesandmacrophages AT poratziv monitoringdistributiondynamicsofevrnacargowithinrecipientmonocytesandmacrophages |