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Role of P2X4/NLRP3 Pathway-Mediated Neuroinflammation in Perioperative Neurocognitive Disorders

Several studies have demonstrated that neuroinflammation is the key to perioperative neurocognitive disorders (PND); however, the specific mechanism postsurgery and anesthesia has not yet been fully clarified. The present study is aimed at exploring the effects of P2X4/NLRP3 signaling pathway in neu...

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Autores principales: Yuan, Hui, Lu, Bo, Ji, Yiqin, Meng, Bo, Wang, Ruichun, Sun, Daofan, Liu, Rongjun, Zhai, Xiaojie, Li, Xiaoyu, Qin, Jinling, Chen, Junping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8825560/
https://www.ncbi.nlm.nih.gov/pubmed/35153623
http://dx.doi.org/10.1155/2022/6355805
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author Yuan, Hui
Lu, Bo
Ji, Yiqin
Meng, Bo
Wang, Ruichun
Sun, Daofan
Liu, Rongjun
Zhai, Xiaojie
Li, Xiaoyu
Qin, Jinling
Chen, Junping
author_facet Yuan, Hui
Lu, Bo
Ji, Yiqin
Meng, Bo
Wang, Ruichun
Sun, Daofan
Liu, Rongjun
Zhai, Xiaojie
Li, Xiaoyu
Qin, Jinling
Chen, Junping
author_sort Yuan, Hui
collection PubMed
description Several studies have demonstrated that neuroinflammation is the key to perioperative neurocognitive disorders (PND); however, the specific mechanism postsurgery and anesthesia has not yet been fully clarified. The present study is aimed at exploring the effects of P2X4/NLRP3 signaling pathway in neuroinflammation and cognitive impairment after surgery. 12–14-month-old male C57BL/6 mice undergoing open tibial fracture surgery by sevoflurane anesthesia were administered P2X4R inhibitor 5-BDBD or saline was intraperitoneally for 3 consecutive days after surgery. Then, the animals were subjected to Morris water maze test or sacrificed to collect the hippocampus. The level of P2X4R and NLRP3 was estimated by Western blot, the activation of microglia was detected via immunohistochemistry, and the expression of TNF-α, IL-1β, and IL-6 was quantified by enzyme-linked immunosorbent assay. These results indicated that tibial surgery caused cognitive impairment, increased the expression of P2X4R and NLRP3, and aggravated the neuroinflammation and microglia activation. However, intraperitoneal injection of 5-BDBD attenuated these effects. In conclusion, these findings indicated that the P2X4/NLRP3 pathway might be involved in the pathophysiology of PND.
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spelling pubmed-88255602022-02-10 Role of P2X4/NLRP3 Pathway-Mediated Neuroinflammation in Perioperative Neurocognitive Disorders Yuan, Hui Lu, Bo Ji, Yiqin Meng, Bo Wang, Ruichun Sun, Daofan Liu, Rongjun Zhai, Xiaojie Li, Xiaoyu Qin, Jinling Chen, Junping Mediators Inflamm Research Article Several studies have demonstrated that neuroinflammation is the key to perioperative neurocognitive disorders (PND); however, the specific mechanism postsurgery and anesthesia has not yet been fully clarified. The present study is aimed at exploring the effects of P2X4/NLRP3 signaling pathway in neuroinflammation and cognitive impairment after surgery. 12–14-month-old male C57BL/6 mice undergoing open tibial fracture surgery by sevoflurane anesthesia were administered P2X4R inhibitor 5-BDBD or saline was intraperitoneally for 3 consecutive days after surgery. Then, the animals were subjected to Morris water maze test or sacrificed to collect the hippocampus. The level of P2X4R and NLRP3 was estimated by Western blot, the activation of microglia was detected via immunohistochemistry, and the expression of TNF-α, IL-1β, and IL-6 was quantified by enzyme-linked immunosorbent assay. These results indicated that tibial surgery caused cognitive impairment, increased the expression of P2X4R and NLRP3, and aggravated the neuroinflammation and microglia activation. However, intraperitoneal injection of 5-BDBD attenuated these effects. In conclusion, these findings indicated that the P2X4/NLRP3 pathway might be involved in the pathophysiology of PND. Hindawi 2022-02-01 /pmc/articles/PMC8825560/ /pubmed/35153623 http://dx.doi.org/10.1155/2022/6355805 Text en Copyright © 2022 Hui Yuan et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yuan, Hui
Lu, Bo
Ji, Yiqin
Meng, Bo
Wang, Ruichun
Sun, Daofan
Liu, Rongjun
Zhai, Xiaojie
Li, Xiaoyu
Qin, Jinling
Chen, Junping
Role of P2X4/NLRP3 Pathway-Mediated Neuroinflammation in Perioperative Neurocognitive Disorders
title Role of P2X4/NLRP3 Pathway-Mediated Neuroinflammation in Perioperative Neurocognitive Disorders
title_full Role of P2X4/NLRP3 Pathway-Mediated Neuroinflammation in Perioperative Neurocognitive Disorders
title_fullStr Role of P2X4/NLRP3 Pathway-Mediated Neuroinflammation in Perioperative Neurocognitive Disorders
title_full_unstemmed Role of P2X4/NLRP3 Pathway-Mediated Neuroinflammation in Perioperative Neurocognitive Disorders
title_short Role of P2X4/NLRP3 Pathway-Mediated Neuroinflammation in Perioperative Neurocognitive Disorders
title_sort role of p2x4/nlrp3 pathway-mediated neuroinflammation in perioperative neurocognitive disorders
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8825560/
https://www.ncbi.nlm.nih.gov/pubmed/35153623
http://dx.doi.org/10.1155/2022/6355805
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