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Untargeted metabolomics-based response analysis of temperature and insecticide exposure in Aedes aegypti

In this study, we utilized an untargeted NMR metabolomics approach to identify the vector response in terms of metabolic profiling after temperature and insecticide exposure in comparison with the control. Clearly, temperature and insecticide exposure cause changes in the underlying metabolism, and...

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Autores principales: Singh, Poonam, Kumar, Pradeep, Pande, Veena, Kumar, Virendra, Dhiman, Ramesh C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8825845/
https://www.ncbi.nlm.nih.gov/pubmed/35136077
http://dx.doi.org/10.1038/s41598-022-05630-z
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author Singh, Poonam
Kumar, Pradeep
Pande, Veena
Kumar, Virendra
Dhiman, Ramesh C.
author_facet Singh, Poonam
Kumar, Pradeep
Pande, Veena
Kumar, Virendra
Dhiman, Ramesh C.
author_sort Singh, Poonam
collection PubMed
description In this study, we utilized an untargeted NMR metabolomics approach to identify the vector response in terms of metabolic profiling after temperature and insecticide exposure in comparison with the control. Clearly, temperature and insecticide exposure cause changes in the underlying metabolism, and the NMR metabolomic profile enables a direct examination of the immediate response of the vector to cope up with these changes. The present study was designed in four parts: A-Aedes aegypti were exposed to 40 °C for one-hour, DDT-4%, malathion-5%, and deltamethrin-0.05% separately and, part B-D; one-hour exposure at 35 °C and 40 °C temperatures followed by one-hour exposure to insecticide. The resultant metabolite profiles were compared with the control. In response to temperature and insecticide exposure, several metabolites and altered pathways were identified. Citrate, maltose, lipids, Nicotinate, Choline, Pyruvate and β-hydroxybutyrate were found as important components of major biological pathways such as tri-carboxylic acid cycle, branched amino acid degradation, glycolysis/gluconeogenesis, amino acid metabolism, lipid and carbohydrate metabolism, nucleotide PRPP pathway, and phospholipid metabolism. Furthermore, the results also suggest that the changes imposed by exposure to temperature and insecticides individually, are reversed with combined exposure, thus negating the impact of each other and posing a threat to the control of Aedes-borne diseases such as dengue, chikungunya, Zika and yellow fever.
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spelling pubmed-88258452022-02-09 Untargeted metabolomics-based response analysis of temperature and insecticide exposure in Aedes aegypti Singh, Poonam Kumar, Pradeep Pande, Veena Kumar, Virendra Dhiman, Ramesh C. Sci Rep Article In this study, we utilized an untargeted NMR metabolomics approach to identify the vector response in terms of metabolic profiling after temperature and insecticide exposure in comparison with the control. Clearly, temperature and insecticide exposure cause changes in the underlying metabolism, and the NMR metabolomic profile enables a direct examination of the immediate response of the vector to cope up with these changes. The present study was designed in four parts: A-Aedes aegypti were exposed to 40 °C for one-hour, DDT-4%, malathion-5%, and deltamethrin-0.05% separately and, part B-D; one-hour exposure at 35 °C and 40 °C temperatures followed by one-hour exposure to insecticide. The resultant metabolite profiles were compared with the control. In response to temperature and insecticide exposure, several metabolites and altered pathways were identified. Citrate, maltose, lipids, Nicotinate, Choline, Pyruvate and β-hydroxybutyrate were found as important components of major biological pathways such as tri-carboxylic acid cycle, branched amino acid degradation, glycolysis/gluconeogenesis, amino acid metabolism, lipid and carbohydrate metabolism, nucleotide PRPP pathway, and phospholipid metabolism. Furthermore, the results also suggest that the changes imposed by exposure to temperature and insecticides individually, are reversed with combined exposure, thus negating the impact of each other and posing a threat to the control of Aedes-borne diseases such as dengue, chikungunya, Zika and yellow fever. Nature Publishing Group UK 2022-02-08 /pmc/articles/PMC8825845/ /pubmed/35136077 http://dx.doi.org/10.1038/s41598-022-05630-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Singh, Poonam
Kumar, Pradeep
Pande, Veena
Kumar, Virendra
Dhiman, Ramesh C.
Untargeted metabolomics-based response analysis of temperature and insecticide exposure in Aedes aegypti
title Untargeted metabolomics-based response analysis of temperature and insecticide exposure in Aedes aegypti
title_full Untargeted metabolomics-based response analysis of temperature and insecticide exposure in Aedes aegypti
title_fullStr Untargeted metabolomics-based response analysis of temperature and insecticide exposure in Aedes aegypti
title_full_unstemmed Untargeted metabolomics-based response analysis of temperature and insecticide exposure in Aedes aegypti
title_short Untargeted metabolomics-based response analysis of temperature and insecticide exposure in Aedes aegypti
title_sort untargeted metabolomics-based response analysis of temperature and insecticide exposure in aedes aegypti
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8825845/
https://www.ncbi.nlm.nih.gov/pubmed/35136077
http://dx.doi.org/10.1038/s41598-022-05630-z
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