Cargando…
The Peroxisomal-CoA Synthetase MoPcs60 Is Important for Fatty Acid Metabolism and Infectious Growth of the Rice Blast Fungus
Fatty acid metabolism is important for the maintenance of fatty acid homeostasis. Free fatty acids, which are toxic in excess, are activated by esterification with coenzyme A (CoA) and then subjected to β-oxidization. Fatty acid β-oxidation-related genes play critical roles in the development and vi...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8826238/ https://www.ncbi.nlm.nih.gov/pubmed/35154208 http://dx.doi.org/10.3389/fpls.2021.811041 |
_version_ | 1784647390880333824 |
---|---|
author | Zhang, Ting Li, Ya-Nan Li, Xue Gu, Wangliu Moeketsi, Emily Kolojane Zhou, Ruiwen Zheng, Xiaobo Zhang, Zhengguang Zhang, Haifeng |
author_facet | Zhang, Ting Li, Ya-Nan Li, Xue Gu, Wangliu Moeketsi, Emily Kolojane Zhou, Ruiwen Zheng, Xiaobo Zhang, Zhengguang Zhang, Haifeng |
author_sort | Zhang, Ting |
collection | PubMed |
description | Fatty acid metabolism is important for the maintenance of fatty acid homeostasis. Free fatty acids, which are toxic in excess, are activated by esterification with coenzyme A (CoA) and then subjected to β-oxidization. Fatty acid β-oxidation-related genes play critical roles in the development and virulence of several phytopathogens. In this study, we identified and characterized a peroxisomal-CoA synthetase in the rice blast fungus Magnaporthe oryzae, MoPCS60, which is a homolog of PCS60 in budding yeast. MoPCS60 was highly expressed during the conidial and early infectious stages and was induced under oleate treatment. Targeted deletion of MoPCS60 resulted in a significant reduction in growth rate when oleate and olive oil were used as the sole carbon sources. Compared with the wild-type strain Guy11, the ΔMopcs60 mutant exhibited fewer peroxisomes, more lipid droplets, and decreased pathogenicity. The distribution of MoPcs60 varied among developmental stages and was mainly localized to peroxisomes in the hyphae, conidia, and appressoria when treated with oleate. Our results suggest that MoPcs60 is a key peroxisomal-CoA synthetase involved in fatty acid β-oxidation and pathogenicity in rice blast fungi. |
format | Online Article Text |
id | pubmed-8826238 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88262382022-02-10 The Peroxisomal-CoA Synthetase MoPcs60 Is Important for Fatty Acid Metabolism and Infectious Growth of the Rice Blast Fungus Zhang, Ting Li, Ya-Nan Li, Xue Gu, Wangliu Moeketsi, Emily Kolojane Zhou, Ruiwen Zheng, Xiaobo Zhang, Zhengguang Zhang, Haifeng Front Plant Sci Plant Science Fatty acid metabolism is important for the maintenance of fatty acid homeostasis. Free fatty acids, which are toxic in excess, are activated by esterification with coenzyme A (CoA) and then subjected to β-oxidization. Fatty acid β-oxidation-related genes play critical roles in the development and virulence of several phytopathogens. In this study, we identified and characterized a peroxisomal-CoA synthetase in the rice blast fungus Magnaporthe oryzae, MoPCS60, which is a homolog of PCS60 in budding yeast. MoPCS60 was highly expressed during the conidial and early infectious stages and was induced under oleate treatment. Targeted deletion of MoPCS60 resulted in a significant reduction in growth rate when oleate and olive oil were used as the sole carbon sources. Compared with the wild-type strain Guy11, the ΔMopcs60 mutant exhibited fewer peroxisomes, more lipid droplets, and decreased pathogenicity. The distribution of MoPcs60 varied among developmental stages and was mainly localized to peroxisomes in the hyphae, conidia, and appressoria when treated with oleate. Our results suggest that MoPcs60 is a key peroxisomal-CoA synthetase involved in fatty acid β-oxidation and pathogenicity in rice blast fungi. Frontiers Media S.A. 2022-01-26 /pmc/articles/PMC8826238/ /pubmed/35154208 http://dx.doi.org/10.3389/fpls.2021.811041 Text en Copyright © 2022 Zhang, Li, Li, Gu, Moeketsi, Zhou, Zheng, Zhang and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Zhang, Ting Li, Ya-Nan Li, Xue Gu, Wangliu Moeketsi, Emily Kolojane Zhou, Ruiwen Zheng, Xiaobo Zhang, Zhengguang Zhang, Haifeng The Peroxisomal-CoA Synthetase MoPcs60 Is Important for Fatty Acid Metabolism and Infectious Growth of the Rice Blast Fungus |
title | The Peroxisomal-CoA Synthetase MoPcs60 Is Important for Fatty Acid Metabolism and Infectious Growth of the Rice Blast Fungus |
title_full | The Peroxisomal-CoA Synthetase MoPcs60 Is Important for Fatty Acid Metabolism and Infectious Growth of the Rice Blast Fungus |
title_fullStr | The Peroxisomal-CoA Synthetase MoPcs60 Is Important for Fatty Acid Metabolism and Infectious Growth of the Rice Blast Fungus |
title_full_unstemmed | The Peroxisomal-CoA Synthetase MoPcs60 Is Important for Fatty Acid Metabolism and Infectious Growth of the Rice Blast Fungus |
title_short | The Peroxisomal-CoA Synthetase MoPcs60 Is Important for Fatty Acid Metabolism and Infectious Growth of the Rice Blast Fungus |
title_sort | peroxisomal-coa synthetase mopcs60 is important for fatty acid metabolism and infectious growth of the rice blast fungus |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8826238/ https://www.ncbi.nlm.nih.gov/pubmed/35154208 http://dx.doi.org/10.3389/fpls.2021.811041 |
work_keys_str_mv | AT zhangting theperoxisomalcoasynthetasemopcs60isimportantforfattyacidmetabolismandinfectiousgrowthofthericeblastfungus AT liyanan theperoxisomalcoasynthetasemopcs60isimportantforfattyacidmetabolismandinfectiousgrowthofthericeblastfungus AT lixue theperoxisomalcoasynthetasemopcs60isimportantforfattyacidmetabolismandinfectiousgrowthofthericeblastfungus AT guwangliu theperoxisomalcoasynthetasemopcs60isimportantforfattyacidmetabolismandinfectiousgrowthofthericeblastfungus AT moeketsiemilykolojane theperoxisomalcoasynthetasemopcs60isimportantforfattyacidmetabolismandinfectiousgrowthofthericeblastfungus AT zhouruiwen theperoxisomalcoasynthetasemopcs60isimportantforfattyacidmetabolismandinfectiousgrowthofthericeblastfungus AT zhengxiaobo theperoxisomalcoasynthetasemopcs60isimportantforfattyacidmetabolismandinfectiousgrowthofthericeblastfungus AT zhangzhengguang theperoxisomalcoasynthetasemopcs60isimportantforfattyacidmetabolismandinfectiousgrowthofthericeblastfungus AT zhanghaifeng theperoxisomalcoasynthetasemopcs60isimportantforfattyacidmetabolismandinfectiousgrowthofthericeblastfungus AT zhangting peroxisomalcoasynthetasemopcs60isimportantforfattyacidmetabolismandinfectiousgrowthofthericeblastfungus AT liyanan peroxisomalcoasynthetasemopcs60isimportantforfattyacidmetabolismandinfectiousgrowthofthericeblastfungus AT lixue peroxisomalcoasynthetasemopcs60isimportantforfattyacidmetabolismandinfectiousgrowthofthericeblastfungus AT guwangliu peroxisomalcoasynthetasemopcs60isimportantforfattyacidmetabolismandinfectiousgrowthofthericeblastfungus AT moeketsiemilykolojane peroxisomalcoasynthetasemopcs60isimportantforfattyacidmetabolismandinfectiousgrowthofthericeblastfungus AT zhouruiwen peroxisomalcoasynthetasemopcs60isimportantforfattyacidmetabolismandinfectiousgrowthofthericeblastfungus AT zhengxiaobo peroxisomalcoasynthetasemopcs60isimportantforfattyacidmetabolismandinfectiousgrowthofthericeblastfungus AT zhangzhengguang peroxisomalcoasynthetasemopcs60isimportantforfattyacidmetabolismandinfectiousgrowthofthericeblastfungus AT zhanghaifeng peroxisomalcoasynthetasemopcs60isimportantforfattyacidmetabolismandinfectiousgrowthofthericeblastfungus |