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Droplet digital PCR assay provides intrahepatic HBV cccDNA quantification tool for clinical application

The persistence of covalently closed circular DNA (cccDNA) poses a major obstacle to curing chronic hepatitis B (CHB). Here, we used droplet digital PCR (ddPCR) for cccDNA quantitation. The cccDNA-specific ddPCR showed high accuracy with the dynamic range of cccDNA detection from 10(1) to 10(5) copi...

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Detalles Bibliográficos
Autores principales:	Hayashi, Sanae, Isogawa, Masanori, Kawashima, Keigo, Ito, Kyoko, Chuaypen, Natthaya, Morine, Yuji, Shimada, Mitsuo, Higashi-Kuwata, Nobuyo, Watanabe, Takehisa, Tangkijvanich, Pisit, Mitsuya, Hiroaki, Tanaka, Yasuhito
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8826402/ https://www.ncbi.nlm.nih.gov/pubmed/35136096 http://dx.doi.org/10.1038/s41598-022-05882-9

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