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The Landscape of Aminoacyl-tRNA Synthetases Involved in Severe Acute Respiratory Syndrome Coronavirus 2 Infection

Aminoacyl-tRNA synthetases (aaRSs) are essential enzymes in translation by linking amino acids onto their cognate tRNAs during protein synthesis. During evolution, aaRSs develop numerous non-canonical functions that expand the roles of aaRSs in eukaryotic organisms. Although aaRSs have been implicat...

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Autores principales: Feng, Yajuan, Tang, Kang, Lai, Qi, Liang, Jingxian, Feng, Min, Zhou, Zhong-Wei, Cui, Haissi, Du, Xiangjun, Zhang, Han, Sun, Litao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8826553/
https://www.ncbi.nlm.nih.gov/pubmed/35153822
http://dx.doi.org/10.3389/fphys.2021.818297
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author Feng, Yajuan
Tang, Kang
Lai, Qi
Liang, Jingxian
Feng, Min
Zhou, Zhong-Wei
Cui, Haissi
Du, Xiangjun
Zhang, Han
Sun, Litao
author_facet Feng, Yajuan
Tang, Kang
Lai, Qi
Liang, Jingxian
Feng, Min
Zhou, Zhong-Wei
Cui, Haissi
Du, Xiangjun
Zhang, Han
Sun, Litao
author_sort Feng, Yajuan
collection PubMed
description Aminoacyl-tRNA synthetases (aaRSs) are essential enzymes in translation by linking amino acids onto their cognate tRNAs during protein synthesis. During evolution, aaRSs develop numerous non-canonical functions that expand the roles of aaRSs in eukaryotic organisms. Although aaRSs have been implicated in viral infection, the function of aaRSs during infections with coronaviruses (CoVs) remains unclear. Here, we analyzed the data from transcriptomic and proteomic database on human cytoplasmic (cyto) and mitochondrial (mt) aaRSs across infections with three highly pathogenic human CoVs, with a particular focus on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We found an overall downregulation of aaRSs at mRNA levels, while the protein levels of some mt-aaRSs and the phosphorylation of certain aaRSs were increased in response to SARS-CoV-2 infection. Strikingly, interaction network between SARS-CoV-2 and human aaRSs displayed a strong involvement of mt-aaRSs. Further co-immunoprecipitation (co-IP) experiments confirmed the physical interaction between SARS-CoV-2 M protein and TARS2. In addition, we identified the intermediate nodes and potential pathways involved in SARS-CoV-2 infection. This study provides an unbiased, overarching perspective on the correlation between aaRSs and SARS-CoV-2. More importantly, this work identifies TARS2, HARS2, and EARS2 as potential key factors involved in COVID-19.
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spelling pubmed-88265532022-02-10 The Landscape of Aminoacyl-tRNA Synthetases Involved in Severe Acute Respiratory Syndrome Coronavirus 2 Infection Feng, Yajuan Tang, Kang Lai, Qi Liang, Jingxian Feng, Min Zhou, Zhong-Wei Cui, Haissi Du, Xiangjun Zhang, Han Sun, Litao Front Physiol Physiology Aminoacyl-tRNA synthetases (aaRSs) are essential enzymes in translation by linking amino acids onto their cognate tRNAs during protein synthesis. During evolution, aaRSs develop numerous non-canonical functions that expand the roles of aaRSs in eukaryotic organisms. Although aaRSs have been implicated in viral infection, the function of aaRSs during infections with coronaviruses (CoVs) remains unclear. Here, we analyzed the data from transcriptomic and proteomic database on human cytoplasmic (cyto) and mitochondrial (mt) aaRSs across infections with three highly pathogenic human CoVs, with a particular focus on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We found an overall downregulation of aaRSs at mRNA levels, while the protein levels of some mt-aaRSs and the phosphorylation of certain aaRSs were increased in response to SARS-CoV-2 infection. Strikingly, interaction network between SARS-CoV-2 and human aaRSs displayed a strong involvement of mt-aaRSs. Further co-immunoprecipitation (co-IP) experiments confirmed the physical interaction between SARS-CoV-2 M protein and TARS2. In addition, we identified the intermediate nodes and potential pathways involved in SARS-CoV-2 infection. This study provides an unbiased, overarching perspective on the correlation between aaRSs and SARS-CoV-2. More importantly, this work identifies TARS2, HARS2, and EARS2 as potential key factors involved in COVID-19. Frontiers Media S.A. 2022-01-26 /pmc/articles/PMC8826553/ /pubmed/35153822 http://dx.doi.org/10.3389/fphys.2021.818297 Text en Copyright © 2022 Feng, Tang, Lai, Liang, Feng, Zhou, Cui, Du, Zhang and Sun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Feng, Yajuan
Tang, Kang
Lai, Qi
Liang, Jingxian
Feng, Min
Zhou, Zhong-Wei
Cui, Haissi
Du, Xiangjun
Zhang, Han
Sun, Litao
The Landscape of Aminoacyl-tRNA Synthetases Involved in Severe Acute Respiratory Syndrome Coronavirus 2 Infection
title The Landscape of Aminoacyl-tRNA Synthetases Involved in Severe Acute Respiratory Syndrome Coronavirus 2 Infection
title_full The Landscape of Aminoacyl-tRNA Synthetases Involved in Severe Acute Respiratory Syndrome Coronavirus 2 Infection
title_fullStr The Landscape of Aminoacyl-tRNA Synthetases Involved in Severe Acute Respiratory Syndrome Coronavirus 2 Infection
title_full_unstemmed The Landscape of Aminoacyl-tRNA Synthetases Involved in Severe Acute Respiratory Syndrome Coronavirus 2 Infection
title_short The Landscape of Aminoacyl-tRNA Synthetases Involved in Severe Acute Respiratory Syndrome Coronavirus 2 Infection
title_sort landscape of aminoacyl-trna synthetases involved in severe acute respiratory syndrome coronavirus 2 infection
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8826553/
https://www.ncbi.nlm.nih.gov/pubmed/35153822
http://dx.doi.org/10.3389/fphys.2021.818297
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