Trapping and structural characterisation of a covalent intermediate in vitamin B(6) biosynthesis catalysed by the Pdx1 PLP synthase

The Pdx1 enzyme catalyses condensation of two carbohydrates and ammonia to form pyridoxal 5-phosphate (PLP) via an imine relay mechanism of carbonyl intermediates. The I(333) intermediate characterised here using structural, UV-vis absorption spectroscopy and mass spectrometry analyses rationalises...

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Detalles Bibliográficos
Autores principales: Rodrigues, Matthew J., Giri, Nitai, Royant, Antoine, Zhang, Yang, Bolton, Rachel, Evans, Gwyndaf, Ealick, Steve E., Begley, Tadhg, Tews, Ivo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8827014/
https://www.ncbi.nlm.nih.gov/pubmed/35360887
http://dx.doi.org/10.1039/d1cb00160d
Descripción
Sumario:The Pdx1 enzyme catalyses condensation of two carbohydrates and ammonia to form pyridoxal 5-phosphate (PLP) via an imine relay mechanism of carbonyl intermediates. The I(333) intermediate characterised here using structural, UV-vis absorption spectroscopy and mass spectrometry analyses rationalises stereoselective deprotonation and subsequent substrate assisted phosphate elimination, central to PLP biosynthesis.

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