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Trapping and structural characterisation of a covalent intermediate in vitamin B(6) biosynthesis catalysed by the Pdx1 PLP synthase

The Pdx1 enzyme catalyses condensation of two carbohydrates and ammonia to form pyridoxal 5-phosphate (PLP) via an imine relay mechanism of carbonyl intermediates. The I(333) intermediate characterised here using structural, UV-vis absorption spectroscopy and mass spectrometry analyses rationalises...

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Detalles Bibliográficos
Autores principales:	Rodrigues, Matthew J., Giri, Nitai, Royant, Antoine, Zhang, Yang, Bolton, Rachel, Evans, Gwyndaf, Ealick, Steve E., Begley, Tadhg, Tews, Ivo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	RSC 2021
Materias:	Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8827014/ https://www.ncbi.nlm.nih.gov/pubmed/35360887 http://dx.doi.org/10.1039/d1cb00160d

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