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Susceptibility of the Western Honey Bee Apis mellifera and the African Stingless Bee Meliponula ferruginea (Hymenoptera: Apidae) to the Entomopathogenic Fungi Metarhizium anisopliae and Beauveria bassiana
This study assessed the nontarget effect of entomopathogenic fungi on the Western honey bee Apis mellifera L. and the African stingless bee Meliponula ferruginea Cockrell (Hymenoptera: Apidae). Pathogenicity of five Metarhizium anisopliae (ICIPE 7, ICIPE 20, ICIPE 62, ICIPE 69, and ICIPE 78) (Metsch...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8827312/ https://www.ncbi.nlm.nih.gov/pubmed/35139218 http://dx.doi.org/10.1093/jee/toab211 |
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author | Omuse, Evanson R Niassy, Saliou Wagacha, John M Ong’amo, George O Lattorff, H Michael G Kiatoko, Nkoba Mohamed, Samira A Subramanian, Sevgan Akutse, Komivi S Dubois, Thomas |
author_facet | Omuse, Evanson R Niassy, Saliou Wagacha, John M Ong’amo, George O Lattorff, H Michael G Kiatoko, Nkoba Mohamed, Samira A Subramanian, Sevgan Akutse, Komivi S Dubois, Thomas |
author_sort | Omuse, Evanson R |
collection | PubMed |
description | This study assessed the nontarget effect of entomopathogenic fungi on the Western honey bee Apis mellifera L. and the African stingless bee Meliponula ferruginea Cockrell (Hymenoptera: Apidae). Pathogenicity of five Metarhizium anisopliae (ICIPE 7, ICIPE 20, ICIPE 62, ICIPE 69, and ICIPE 78) (Metschnikoff) Sorokin (Hypocreales: Clavicipitaceae) and one of Beauveria bassiana (ICIPE 284) (Balsamo) Vuillemin (Hypocreales: Cordicipitaceae) isolates were evaluated on bees at 10(8) conidia/ml. Conidial acquisition was evaluated immediately after exposure. Apis mellifera acquired more conidia (2.8 × 10(4)–1.3 × 10(5) conidia per bee) compared to M. ferruginea (1.1 × 10(4)–2.3 × 10(4) conidia per bee). In the bioassay with A. mellifera, ICIPE 7, ICIPE 20, and ICIPE 69 moderately reduced the survival by 16.9, 17.4, 15.3%, with lethal times LT(10) = 7.4, 7.6, 8.1 d and LT(25) = 8.7, 10.0, 9.9 d, respectively. The three isolates caused A. mellifera mycosis of 11.6–18.5%. None of the isolates had a significant effect on M. ferruginea. The tested isolates are nontoxic to bees according to the International Organization of Biological Control (IOBC) classification. However, the effect of ICIPE 7, ICIPE 20, and ICIPE 69 merits further studies on bee colonies, especially those of A. mellifera, under field conditions. |
format | Online Article Text |
id | pubmed-8827312 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-88273122022-02-10 Susceptibility of the Western Honey Bee Apis mellifera and the African Stingless Bee Meliponula ferruginea (Hymenoptera: Apidae) to the Entomopathogenic Fungi Metarhizium anisopliae and Beauveria bassiana Omuse, Evanson R Niassy, Saliou Wagacha, John M Ong’amo, George O Lattorff, H Michael G Kiatoko, Nkoba Mohamed, Samira A Subramanian, Sevgan Akutse, Komivi S Dubois, Thomas J Econ Entomol Biological and Microbial Control This study assessed the nontarget effect of entomopathogenic fungi on the Western honey bee Apis mellifera L. and the African stingless bee Meliponula ferruginea Cockrell (Hymenoptera: Apidae). Pathogenicity of five Metarhizium anisopliae (ICIPE 7, ICIPE 20, ICIPE 62, ICIPE 69, and ICIPE 78) (Metschnikoff) Sorokin (Hypocreales: Clavicipitaceae) and one of Beauveria bassiana (ICIPE 284) (Balsamo) Vuillemin (Hypocreales: Cordicipitaceae) isolates were evaluated on bees at 10(8) conidia/ml. Conidial acquisition was evaluated immediately after exposure. Apis mellifera acquired more conidia (2.8 × 10(4)–1.3 × 10(5) conidia per bee) compared to M. ferruginea (1.1 × 10(4)–2.3 × 10(4) conidia per bee). In the bioassay with A. mellifera, ICIPE 7, ICIPE 20, and ICIPE 69 moderately reduced the survival by 16.9, 17.4, 15.3%, with lethal times LT(10) = 7.4, 7.6, 8.1 d and LT(25) = 8.7, 10.0, 9.9 d, respectively. The three isolates caused A. mellifera mycosis of 11.6–18.5%. None of the isolates had a significant effect on M. ferruginea. The tested isolates are nontoxic to bees according to the International Organization of Biological Control (IOBC) classification. However, the effect of ICIPE 7, ICIPE 20, and ICIPE 69 merits further studies on bee colonies, especially those of A. mellifera, under field conditions. Oxford University Press 2021-11-22 /pmc/articles/PMC8827312/ /pubmed/35139218 http://dx.doi.org/10.1093/jee/toab211 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Entomological Society of America. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Biological and Microbial Control Omuse, Evanson R Niassy, Saliou Wagacha, John M Ong’amo, George O Lattorff, H Michael G Kiatoko, Nkoba Mohamed, Samira A Subramanian, Sevgan Akutse, Komivi S Dubois, Thomas Susceptibility of the Western Honey Bee Apis mellifera and the African Stingless Bee Meliponula ferruginea (Hymenoptera: Apidae) to the Entomopathogenic Fungi Metarhizium anisopliae and Beauveria bassiana |
title | Susceptibility of the Western Honey Bee Apis mellifera and the African Stingless Bee Meliponula ferruginea (Hymenoptera: Apidae) to the Entomopathogenic Fungi Metarhizium anisopliae and Beauveria bassiana |
title_full | Susceptibility of the Western Honey Bee Apis mellifera and the African Stingless Bee Meliponula ferruginea (Hymenoptera: Apidae) to the Entomopathogenic Fungi Metarhizium anisopliae and Beauveria bassiana |
title_fullStr | Susceptibility of the Western Honey Bee Apis mellifera and the African Stingless Bee Meliponula ferruginea (Hymenoptera: Apidae) to the Entomopathogenic Fungi Metarhizium anisopliae and Beauveria bassiana |
title_full_unstemmed | Susceptibility of the Western Honey Bee Apis mellifera and the African Stingless Bee Meliponula ferruginea (Hymenoptera: Apidae) to the Entomopathogenic Fungi Metarhizium anisopliae and Beauveria bassiana |
title_short | Susceptibility of the Western Honey Bee Apis mellifera and the African Stingless Bee Meliponula ferruginea (Hymenoptera: Apidae) to the Entomopathogenic Fungi Metarhizium anisopliae and Beauveria bassiana |
title_sort | susceptibility of the western honey bee apis mellifera and the african stingless bee meliponula ferruginea (hymenoptera: apidae) to the entomopathogenic fungi metarhizium anisopliae and beauveria bassiana |
topic | Biological and Microbial Control |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8827312/ https://www.ncbi.nlm.nih.gov/pubmed/35139218 http://dx.doi.org/10.1093/jee/toab211 |
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